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A specific antigen from group A streptococcal cell membrane and 1% PHA used as a stimulant to test the peripheral blood lymphocyte adherence inhibition percent and procoagulant activity in 30 cases of active rheumatic carditis, 24 cases of inactive rheumatic carditis and 38 cases of normal controls.

用A组乙型溶血链球菌特异性菌膜抗原和1%植物血凝素作为刺激物,分别测定38例健康对照组,30例风湿性心脏病风湿活动组,24例风湿性心脏病静止组的外周血淋巴细胞贴壁抑制百分率(Lymphocyte adherence inhibition percent, LAIP)和促凝血活性(Procoagulant activity, PCA)。

In the first section, the effect of sodium houttuyfonate on the level of lysozyme secreted by macrophages was first tested by bacteriolytic method; then the effect of sodium houttuyfonate on the level of tartaric acid resistant acid phosphatase produced in macrophages was investigated by analyzing the phenol generated in the reaction that was catalyzed by the enzyme; the third, the effect of sodium houttuyfonate on the phagocytic activity of macrophages was observed by microscopy; the forth, the effect of sodium houttuyfonate on the production of complement C4 from macrophages was tested by hemolytic method; the fifth, the effect of sodium houttuyfonate on the expression of C3b receptors on the surface of macrophages was analyzed by observing the formation percentage of zymosan wreath; the sixth, the effect of sodium houttuyfonate on the respiratory burst in macrophages was tested by flow cytometry; the seventh, the effect of sodium houttuyfonate on the production of IL-1α and IL-1β from macrophages was tested by ELISA method.

在这一章中,首先,采用溶菌法考察合成鱼腥草素对巨噬细胞分泌溶菌酶水平的影响;其次,采用游离酚法研究合成鱼腥草素对巨噬细胞产生的耐酒石酸酸性磷酸酶水平的影响;第三,采用显微镜观察法考察合成鱼腥草素对巨噬细胞吞噬功能的影响;第四,采用溶血法测定合成鱼腥草素对巨噬细胞产生的补体C4水平的影响;第五,采用酵母花环法检测合成鱼腥草素对巨噬细胞表面C3b受体水平的影响;第六,采用流式细胞术法观察合成鱼腥草素对巨噬细胞呼吸爆发的影响;第七,采用ELISA法探讨合成鱼腥草素对巨噬细胞产生IL-1α及IL-1β水平的影响。

And the effect of the percentage of hydrochloric acid to acetone,the volume ratio of hydrochloric acid acetone to hematolysis solution were researched.Results:The supersonic wave hemolysis was Established.

结果:确立超声波法进行红细胞溶血,36.5%浓盐酸与丙酮的体积为1%的血红素抽提液,与溶血液的体积比为5∶1抽提10分钟,最终可从新鲜猪血中得到红素6.0 g/L,纯度达99.8%。

Methods:The fresh porcine blood added sodium citrate was used for extracting of heme. The methods of hematolysis by water,ethanol and supersonic wave were compared.

新鲜抗凝猪血为原料,比较了水溶胀法、乙醇法以及超声波法对红细胞溶血效果,以盐酸丙酮配比,盐酸丙酮与溶血液体积比对猪血中血红素提取的影响。

Clindamycin Phosphate for Injection is reconstituted in 5% Glucose Injection to a concentration of 3% for the haemolysis on red cells.

以5%葡萄糖注射液配制的3%克林霉素磷酸酯溶液对红细胞的溶血作用,结果未见出现溶血或凝集现象。

A rupture of the cell membrane, release of its cell content.therefore, animal cells other than red blood cell cannot carry out "haemolysis".

溶血作用包括红血球的:细胞膜破裂;放出它内含的血红素;因此除了红血球以外的其他细胞是没有&溶血作用&的。

Don at a high concentration(300 mg/kg) can significantly enhance the content of haemolysin in mice body.

在实验浓度范围内,半枝莲多糖对免疫抑制小鼠腹腔巨噬细胞吞噬功能未见明显增强效果;大剂量(300 mg/kg)半枝莲多糖能显著提高血清溶血素抗体水平,与是否脱蛋白无关。

In this paper, the principle of preparation of live vaccine with Saccharomyces cerevisiae by using the surface-display technique, the advantage of displaying protein on surface of the yeast cell, and surface-display of haemolysin from Vibrio harveyi on the yeast cells and their potential applications as live vaccine in marine fish are reviewed.

本文对利用酒精酵母细胞表面展示技术制备活疫苗的基本原理,在酵母细胞表面展示蛋白质的优点,以及哈维氏弧菌溶血素蛋白在酵母菌细胞表面上展示及其作为活疫苗的潜在应用作了综述。

The polysaccharides in Scutellaria barbata D.Don at a high concentration (300 mg/kg) can significantly enhance the content of haemolysin in mice body. Conclusion: The optimal process was applicable to the extraction of polysaccharides from Scutellaria barbata D.

在实验浓度范围内,半枝莲多糖对免疫抑制小鼠腹腔巨噬细胞吞噬功能未见明显增强效果;大剂量(300 mg/kg)半枝莲多糖能显著提高血清溶血素抗体水平,与是否脱蛋白无关。

ResultsIn the immune deficit mice ,1.33g/kg Naoshu capsule could enhance the phagocytic activity of monocytes, 0.67g/kg and 1.33g/kg Naoshu capsule could increase content of haemolysin antibody,and decrease footpad swelling induced by cyclophosphamide.

结果1.33g/kg脑舒胶囊能提高免疫低下小鼠单核巨噬细胞吞噬功能;0.67g/kg和1.33g/kg脑舒胶囊能增加免疫低下小鼠溶血素抗体生成,并拮抗环磷酰胺诱导的免疫低下小鼠足垫肿胀度减轻。

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