溶血素

But we have seldom found the reports about it in China; So writer summarized some literature more than twenty years; name,classification and family of hemolysin have been written, especially three of representative species:α-toxin, Streptolysin O and HlyA; Nowaday, A relationship between the hemolysin and pathopoiesis of bacterium is obvious.

另外，细菌溶血素的英文命名并不统一，检索时容易造成漏检或误检；通过查阅大量英文文献资料，归纳汇总近20年报道的溶血素研究进程；该文详细列出了细菌溶血素名称、分类以及各溶血素家族列表。并且分别介绍了其中三种典型的穿孔毒素：葡萄球菌α-毒素、链球菌溶血素-O、埃希氏大肠杆菌溶血素HlyA。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因，即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联，得到三联融合毒素基因tdh-vvhA-vmhA。

Methods: K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a self-made lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14(superscript +), CD45(superscript +) on lymphocytes, monocytes and granulocytes were detected.

应用流式细胞术对40份K3 EDTA抗凝外周血分别用自制流式细胞仪专用溶血素与FACS溶血素处理后，检测其对血液红细胞的溶解效果及对淋巴细胞、单核细胞和粒细胞表面特异性抗原CD14、CD45表达率的影响。

Methods: K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a selfmade lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14+, CD45+ on lymphocytes, monocytes and granulocytes were detected.

应用流式细胞术对40份K3 EDTA抗凝外周血分别用自制流式细胞仪专用溶血素与FACS溶血素处理后，检测其对血液红细胞的溶解效果及对淋巴细胞、单核细胞和粒细胞表面特异性抗原CD14+、CD45+表达率的影响。

This study will help us to identify the role of vhh/tlh haemolysin gene in the pathogenicity of vibrios.

对vhh/tlh溶血素基因在弧菌中的分布研究，将有助于进一步确定这类溶血素基因在病原弧菌致病性中的作用。

HEPS could increase the humoral and cellular immune functions in mice.200mg/kg HEPS showed the best effect,the effect of 400mg/kg group was no better than that of 200mg/kg group.

本文通过炭粒廓清实验及溶血素实验来检测HEPS对小鼠网状内皮系统吞噬异物能力和溶血素抗体生成的影响。1 材料与

Previously, it was found that the hemolytic activity of the extracellular product of V. harveyi was involved in pathogenesis in salmonids, and the most pathogenic isolate, VIB 645, contains two closely related hemolysin genes, i.e. vhhA and vhhB.

已发现哈维氏弧菌对鱼类的致病性与其分泌的胞外产物中的溶血素相关，致病力最强的菌株VIB 645有2个碱基序列非常相似的溶血素基因，vhhA和vhhB。

The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

A VHH probe, which is specific to V. harveyi vhhA haemolysin gene, was used to screen EcoR Ⅰ digests of total DNA from 57 vibrio strains, including 26 vibrio type strains, 20 V.

哈维氏弧菌的VHH溶血素基因与副溶血弧菌(V.parahaemolyticus)的TLH热不稳定性溶血素基因具有高度相似性，其氨基酸序列的相似性达到85.6﹪。

METHODS:Observe the effect of SKR on immunity function in mice,the phagocytotic rate and phagocytotic index,haemolysin in serum,plaque forming cell,and the proliferation of splenocyte following administration SKR for 15 days.

以SK初乳素R-1连续灌胃给药15 d，测定小鼠增强小鼠炭末廓清能力、小鼠血清溶血素抗体水平、血清IL-2含量、抗体生成细胞数、脾淋巴细胞增殖能力等。结果：SK初乳素R-1各剂量组能增强小鼠炭末廓清率和廓清指数，各剂量组均能明显升高小鼠血清溶血素抗体水平(P.01)，提高小鼠血清IL-2含量(P.01)，促进小鼠抗体细胞形成(P.01)，增强ConA诱导的脾淋巴细胞增殖能力(P.01)。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。