溶血的

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

And Bauhinia purpurea could inhibit vibrio. And the extracts, extrated with petroleum ether from leaves of Melia azedarach L. and Bauhinia purpurea can inhibit Vibrio parahaemolyticus, too. The remainder extracts of Mangifera indica Linn.showed strong inhibition effect on Vibrio tubiashii. While the extracts of Eriobotrya japonica showed no inhibition effect.

结果 夹竹桃叶、芒果叶、苦楝叶和羊蹄甲叶4种中草药乙酸乙酯萃取部分对供试弧菌有很好的抑菌作用；苦楝叶和羊蹄甲叶石油醚萃取部分对副溶血弧菌有抑菌作用；芒果叶的水溶性部分对塔氏弧菌有很强的抑菌作用；枇杷叶提取物对4株弧菌均无抑制作用。

Methods Used microplate three-stage polybrene test,bromelin technology,the anti-globulin test and the traditional test tube,paralleled determines the titre of Rh,MNSs,Duffy,Kidd blood system irregular antibody,observed the sensitivity and specificity of assay,selected the best screens test method,and observed influence of the...

方法采用微量板聚凝胺三步法试验、菠萝酶法、抗人球蛋白法及经典试管TSPT，平行检测一批Rh等抗体效价，选出最佳的筛查方法，并观察抗凝剂、溶血、脂血对实验的影响。

Methods The sensitivity of antibiotics and hemolysin of isolated 302 strains of V.parahaemolyticus were detected during two years,and the clinical data were analyzed statistically.

方法对两年中分离到的302株副溶血性弧菌进行溶血素和抗生素敏感性测定，并对患者的临床资料进行统计分析。

The structure of ungrafted and grafted films was characterized by FTIR spectroscopy respectively, and their blood compatibility was measured by water absorbency rate,hemolysis test, recalcification time test and static platelet adhesion experiment.

接枝前后聚氨酯膜的结构及抗凝血性能用FTIR、吸水率测试、溶血实验、复钙实验、血小板黏附试验等进行了表征。

Methods: PNS was got by extracting panax notognseng with general method of alcohol circumfluence, purified with organic solvents, and separated with D101 macroreticular resin. The hematolysis rate and the immunocompetence of biology of PNS were determined by hemolysis test and experiment of splenocyte proliferation respectively.

乙醇回流法提取PNS粗提物，经有机溶剂除杂后，通过大孔树脂柱层析分离纯化；溶血性试验测定PNS作用后红细胞的溶血率；脾淋巴细胞增殖反应测定PNS的生物免疫活性。

The biocompatibility of self-designed hydroxyapatite/poly rods was evaluated both in vitro and in vivo including Ames test, micronucleus test, acute and subacute systemic toxicity test, hemolysis test, hemopexis test and long-term muscle and bone implant test.

对自行研制的可吸收羟基磷灰石/聚DL-乳酸复合骨折内固定材料进行了一系列生物相容性实验，包括Ames实验、微核实验、急性和亚急性毒性实验、溶血实验、凝血试验和肌肉、骨内长期植入实验。

The biocompatibility of self-designed hydroxyapatite/poly rods was evaluated both in vitro and in vivo including Ames test, micronucleus test, acute and subacute systemic toxicity test, hemolysis test, hemopexis test and long-term muscle and bone implant test.

对自行研制的可吸收羟基磷灰石/聚DL-乳酸复合骨折瘦身内固定材料进行了一系列生物相容性实验，包括Ames实验、微核实验、急性和亚急性毒性实验、溶血实验、凝血试验和肌肉乙肝、骨内长期植入实验。

The recombinant E. coli revealed a strong hemolytic activity on fish blood agar plate and phospholipase activity on egg yolk emulsion plate.

重组菌在鱼血平板上表现出很强的溶血活性，并且在卵磷脂平板上表现出磷脂酶活性。

Based on the colony morphology and the hemolytic activity on trypticase soy-sheep blood agar plate of isolates, B. cereus group bacteria could be highly selected on MYP and PEMBA medium.

通过对细菌菌落形态和生长状况分析，结合血琼脂平板上的溶血实验，利用这两种培养基可以很好地从芽胞杆菌中选择出Bc群微生物。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐