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The results showed that under different doses the hemolytic index of the gelatin microspheres was lower than 5%, so it did not have significant toxicity; primary skin irritation test showed there were no erythema, eschar and edema of 10% florfenicol gelatin microspheres injection; the LD50 of the microspheres was 2.226 7 g/kg and its 95% credibility limit was 1.950 2~2.635 6 g/kg.

实验结果显示,不同剂量的肺靶向氟苯尼考明胶微球注射剂的溶血指数均小于5%,无明显毒性;10%氟苯尼考明胶微球注射剂对受试动物皮肤无明显刺激作用;肺靶向氟苯尼考明胶微球对小鼠的LD50为2.226 7 g/kg,LD50 95%的可信限为1.950 2 ~2.635 6 g/kg。

With rabbit antiserum against ECP of reference strain JEL4,ECPs of 25 Et strains were detected by Dot-ELISA; additionally,alI strains had been detected both on their virulent factor Excellular Products, including the hemolysin and extracellular protease, and on their pathogenicity to mice and Xiphophorus helleri.

以Et强致病株JEL4的胞外产物ECP为抗原制备兔抗血清,用于Dot-ELISA检测25株Et的ECP,同时对Et的溶血素和胞外蛋白酶进行检测,结合小鼠和剑尾鱼的致病性试验。

Using the known sequence of hemolysin gene of V. anguillarum PT84057, the hemolysin gene of V. anguillarum M3 could not be amplified.

用已发表的血清型为 A 的鳗弧菌 PT84057 的序列设计引物,没有扩增出鳗弧菌 M3 的溶血素基因。

While erthrocyte, hemoglobin and Hela cell were under oxidative damage induced by the hydrogen peroxide (H2O2) or tert-butyl hydroperoxide on different concentrations of dihydromyricetin, hemolysis rate of erythrocyte and methemoglobin converted from hemoglobin was estimated by ultraviolet-visible absorption spectroscopy. Whereas mmalondialdehyde from erythrocyte was measured by thiobarbituric acid colorimetry, the cellular viability of Hela cell was examined using MTT 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay.

在不同的二氢杨梅素浓度下,用过氧化氢或过氧化特丁烷对红细胞、血红蛋白和Hela细胞进行氧化伤害,以紫外-可见光分光光度法检测红细胞溶血率和由正常血红蛋白生成的高铁血红蛋白,硫代巴比妥酸法测定红细胞中的丙二醛,MTT法检测Hela细胞的活力。

Can scavenge DPPH·strongly with IC_(50) of 0.164 mg/mL, and inhibit hemolysis of mice erythrocyte evidently with IC_(50) of 0.112 mg/mL. The reactive oxygen species resistant power of mice serum is enhanced by the pigment, the concentration of MDA resulting from peroxidation of lipid in mice liver tissue can be reduced, and the swelling of mice liver mitochondria can also be restrained. The preventing experiments of the pigment of Lycium ruthenicum Murr.

在药理学研究中体外抗氧化实验表明,PLR具有一定的抗氧化能力,表现出很强的DPPH·自由基清除活性,IC_(50)达到0.164 mg/mL;能显著的抑制小鼠红细胞溶血,IC_(50)为0.112mg/mL;能增强小鼠血清抗活性氧能力,抑制小鼠肝组织脂质过氧化产物MDA生成和小鼠肝线粒体肿胀度,并显示出一定的量效关系。

According to Pharmacopoeia of the Peoples Republic of China and the relevant technical provisions of TCM, natural medicine, injection intermediates were tested by quality and safety tests. The results of acute toxicity test, local muscle stimulation test, sensitivity test, hemolysis test proved the injection of polysaccharide extracted from ABM was safe and can be used via intramuscular injection and intravenous injection.

在上述研究的基础上对姬松茸多糖注射液的生产工艺进行了设计,初步制得注射液成品,根据《中华人民共和国药典》和《中药、天然药物相关的技术指导原则》对注射液的中间体进行质量安全性试验:急性毒性试验、肌肉局部刺激试验、过敏试验和溶血试验,试验结果均符合相关规定,表明姬松茸多糖注射液可以用于肌肉注射和静脉注射,无毒副作用,不会产生不良后果。

Background— Numerous in itro studies suggest that sphingosine 1-phosphate (S1P), a bioactie lysosphingolipid associated with high-density lipoproteins, accounts at least partly for the potent antiinflammatory properties of high-density lipoprotein and, thereby, contributes to the antiatherogenic potential attributed to high-density lipoproteins.

背景——众多的活体研究表明鞘氨醇1-磷酸盐作为一种与高密度脂蛋白相关的生物活性溶血磷脂,至少是高密度脂蛋白有力抗炎特质的部分原因,从而促成高密度脂蛋白的抗动脉粥样硬化潜能。

Objective:To explore the changes of plasma Lysophatisic acid level in patients with transient ischemic attacks or cerebric infrction.

溶血磷脂酸是磷脂中的一种小分子物质,是磷脂生物合成早期阶段的关键性前体,在体内的信号传递中起着十分重要的作用,因此被称为多功能&磷脂信使&[1]。

Objective:To explore the changes of plasma Lysophatisic acid level in patients with transient ischemic attacks or cerebric infrction. And to value the effect of those in the formation and progress of those diseases.

溶血磷脂酸是磷脂中的一种小分子物质,是磷脂生物合成早期阶段的关键性前体,在体内的信号传递中起着十分重要的作用,因此被称为多功能&磷脂信使&[1]。

Methods 1. The mice were divided into control and 3 Ganodermalucidum spores oil groups (at low, moderate and high doses), orally given forconsecutive 30 days. The body, spleen, thymus weight were observed, study on theeffect of the drug on the cellular immune function by proliferation and transformationof spleen lymphocytes test; study on the effect of the drug on the humoral immunefunction of mice by serum erythrocytolysin test; study on the effect of the drug on themononuclear macrophage function by carbon clearance and neutral red test.

实验方法 1设玉米油对照组和低、中、高三个灵芝孢子油剂量组,连续灌胃30天后观察小鼠体重、胸腺、脾脏重量指数;采用吞噬中性红和碳廓清实验测定对小鼠单核-巨噬细胞功能的影响;血清溶血素实验测定对小鼠体液免疫功能的影响;ConA诱导脾淋巴细胞转化实验测定对小鼠细胞免疫功能的影响;MTT法测定对小鼠NK细胞杀伤活性、脾细胞产生TNF-α和IL-2含量的影响。

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