溶血

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

And Bauhinia purpurea could inhibit vibrio. And the extracts, extrated with petroleum ether from leaves of Melia azedarach L. and Bauhinia purpurea can inhibit Vibrio parahaemolyticus, too. The remainder extracts of Mangifera indica Linn.showed strong inhibition effect on Vibrio tubiashii. While the extracts of Eriobotrya japonica showed no inhibition effect.

结果 夹竹桃叶、芒果叶、苦楝叶和羊蹄甲叶4种中草药乙酸乙酯萃取部分对供试弧菌有很好的抑菌作用；苦楝叶和羊蹄甲叶石油醚萃取部分对副溶血弧菌有抑菌作用；芒果叶的水溶性部分对塔氏弧菌有很强的抑菌作用；枇杷叶提取物对4株弧菌均无抑制作用。

Methods Using Vibrio eoralliilyticus, Vibrio tubiashii, Vibrio parahaemolyticus and Vibrio earehariae as the tested bacteria, the inhibition zone and the minimal inhibitory concentration were measured. Results The extracts, extracted with ethyl acetate from the leaves of Nerium indicum Mill., Mangifera indica Linn., Melia azedarach L.

采用纸片法和试管法，以溶珊瑚弧菌、塔氏弧菌、副溶血弧菌、鲨鱼弧菌为供试菌株，测定中草药不同极性萃取部分的抑菌圈和最小抑菌浓度。

Methods Using Vibrio coralliilyticus, Vibrio tubiashii, Vibrio parahaemolyticus and Vibrio carchariae as the tested bacteria, the inhibition zone and the minimal inhibitory concentrationwere measured. Results The extracts, extracted with ethyl acetate from the leaves of Nerium indicum Mill., Mangifera indica Linn., Melia azedarach L.

采用纸片法和试管法，以溶珊瑚弧菌、塔氏弧菌、副溶血弧菌、鲨鱼弧菌为供试菌株，测定中草药不同极性萃取部分的抑菌圈和最小抑菌浓度。

The results show that: This tablet can decrease the rat"s footpad swell induced by Freud"s, inhibit the permeability of the blood capillary in inflammatory joint and decrease the 11-2 level in serum while increasing the rat"s body weight,(2) It can lower the rat"s temperature and the permeability of the blood capillary in inflammatory joint induced by collagen and inhibit the delayed type hypersensitivity,It can lower the stickiness of full blood and the accumulation of erythrocyte obviously inblood stasis model rats,lt can inhibit the formation of hemolysin and decrease the index of carbon granule clearance in mice, It can lower the permeability of celiac blood capillary and inhibit the granulomatous hyperplasia by sc cotton ,It can inhibit the pain induced by acetic acid and raise the pain threshold value of heat stimulation.

结果表明：关络通片能明显降低佐剂性关节炎大鼠左右足趾肿胀度，降低大鼠炎症关节的通透性，抑制其IL-2含量，对大鼠体重增长缓慢有一定的改善作用；能改善Ⅱ型胶原诱导性关节炎大鼠的表征指标及炎症关节的通透性，对其迟发型超敏反应有一定的抑制作用；对血瘀模型大鼠，能显著性降低其全血粘度、血浆粘度、红细胞压积；能抑制小鼠的溶血素抗体生成，抑制小鼠网状内皮系统的吞噬能力，具有一定的抑制机体免疫功能的作用，能抑制小鼠腹腔毛细血管通透性增高，抑制大鼠棉球肉芽组织增生，具有一定的抗炎作用；能抑制小鼠醋酸扭体反应、提高小鼠热板痛阈值，具有一定的镇痛作用。

Methods The peripheral blood lymphocytes,sera of 55 patients with rheumatic carditis and a specific antigen from group A streptococcal cell membrane were prepared and used as stimulants to test the heart tissue cell procoagulant activity in culture.

用风湿性心脏炎患者的外周血淋巴细胞、血清及A组乙型溶血链球菌膜抗原作为刺激物，测定体外培养的人胚心肌细胞促凝血活性。

HPCA was measured by using a one-stage plasma recalcification clotting time.Results The peripheral blood lymphocytes from rheumatic carditis patients can stimulate the procoagulant activity of human heart tissue cell in vitro.But the sera of rheumatic carditis patients and group A streptococcal cell membrane can not increase HPCA.

结果 患者的外周血淋巴细胞能使HPCA明显升高；静止期风心病、风湿性关节炎、其他疾病对照组及健康对照组患者的外周血淋巴细胞均无此特性，迁延活动型风湿性心脏炎患者的血清及A组乙型溶血链球菌膜抗原对HPCA也无直接影响。

The results show that the synthetic compounds conform to the target compounds and the primary bioassay for anti-vibrio activity displays that the pyrrole alkaloids have a weak inhibition against Vibrio parahaemolyticus and Vibrio alginolyticus compared with the positive control of chloromycetin.

结果表明，所合成的化合物与目标化合物结构相一致。初步的抗溶藻弧菌和副溶血弧菌活性筛选表明，合成的吡咯生物碱具有一定的抗弧菌活性，但相对于阳性对照药氯霉素来说，合成的绝大部分吡咯生物碱抗弧菌活性较弱。

The presence of hemosiderin granules and absence of haptoglobin indicates a severe acute intravascular hemolytic episode about 2 to 3 days ago.

尿中血铁质颗粒的存在以及血中结合珠蛋白缺失，表明大约2-3天前该病人发生过严重的急性血管内溶血。

Methods: In all the SA, blood test, serum ferritin, folacin and vitamin B12 levels and bone marrow cytology examination were done. Then gastroscopy, colonoscopy, liver and kidney function test, hemolytic laboratory indicators, serum thyroid studies and serological markers of viral hepatitis were done as needed.

对140例重度贫血患者均行血常规、铁蛋白、叶酸与维生素B12测定及骨髓细胞学检查，然后根据病情选择性行胃肠镜、肝肾功能、溶血方面实验室检查及甲状腺功能、肝炎标志物、出凝血常规检测等。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。