溶血

Abstract］ Objective: To observe the influence of ephedran on erythrocytolysin antibody formation of mouse caused by sheep erythrocyte and explore the effect of ephedran on the immune function of the mouse.

目的：观察麻黄多糖对绵羊红细胞所致小鼠溶血素生成的影响，以了解麻黄多糖对正常小鼠免疫功能的作用。

Results 6.3 mg/kg diazinon decreased the number of IgMplaque forming cell,blood serum erythrocytolysin level and DTH to SRBC.12.5,25 mg/kg dizainon could decrease the relative weight of thymus at about 20%,the number of IgMplaque forming cell at about 39% and 52%,the blood serum erythrocytolysin level at about 15% and 20%,the DTH to SRBC at about 33% and 35%,the ability of T lymphocyte proliferation at about 27% and 35%,the activity of NK cell at about 24% and 28%,respectively.

结果 敌匹硫磷染毒4周后，6.3 mg／kg组小鼠的体液免疫功能和迟发型变态反应受到抑制；12.5，25 mg/kg组小鼠的胸腺脏器系数均降低20％，抗体生成细胞数分别降低39%和52％，血清溶血素水平分别降低15％和20％，迟发型变态反应水平分别降低33％和35％，淋巴细胞增殖能力分别下降27％和35％，NK细胞活性分别下降24％和28％。

Methods 1. The mice were divided into control and 3 Ganodermalucidum spores oil groups (at low, moderate and high doses), orally given forconsecutive 30 days. The body, spleen, thymus weight were observed, study on theeffect of the drug on the cellular immune function by proliferation and transformationof spleen lymphocytes test; study on the effect of the drug on the humoral immunefunction of mice by serum erythrocytolysin test; study on the effect of the drug on themononuclear macrophage function by carbon clearance and neutral red test.

实验方法 1设玉米油对照组和低、中、高三个灵芝孢子油剂量组，连续灌胃30天后观察小鼠体重、胸腺、脾脏重量指数；采用吞噬中性红和碳廓清实验测定对小鼠单核-巨噬细胞功能的影响；血清溶血素实验测定对小鼠体液免疫功能的影响；ConA诱导脾淋巴细胞转化实验测定对小鼠细胞免疫功能的影响；MTT法测定对小鼠NK细胞杀伤活性、脾细胞产生TNF-α和IL-2含量的影响。

The immune function was evaluated with T lymphocyte proliferation,NK cell activity assay,IgMplaque forming cell assay,blood serum erythrocytolysin assay and delayedtypehypersensitivity to SRBC.

采用T淋巴细胞增殖和迟发型变态反应来评价细胞免疫功能，采用血清溶血素测定和抗体生成细胞检测来评价体液免疫功能，采用乳酸脱氢酶法测定自然杀伤细胞活性来评价非特异性免疫功能。

The acute toxicity andmain organs of pathological section for 30 days were observed. Result 1. Ganoderma lucidum spores oil at 0.20g/kg, 0.40g/kg, 1.20g/kg dose couldsignificantly promote the lymphocyte transformation, raise clearance index numberand phagocytic index number, enhance the activity of NK cells and the level of TNF-α, IL-2 in spleen. Ganoderma lucidum spores oil at high dose and middle dose couldsignificantly enhance the serum erythrocytolysin level.

实验结果 1低、中、高灵芝孢子油组(0.20g／kg，0.40g／kg，1.20g／kg)均可提高小鼠单核-巨噬细胞的吞噬指数及碳廓清指数，促进ConA诱发脾淋巴细胞转化，增强小鼠NK细胞活性，提高脾细胞产生TNF-α和IL-2的能力；高、中剂量灵芝孢子油可提高小鼠的血清溶血素水平。

METHOD: The following tests were made:①test with of hemopoietic inhibition induced by cyclophosphamide on mice,②Carban particles clearance test,③Erythrolysin formetien test,④Swimming test,⑤Hypoxia test,⑥Sedation test,⑦Hypnosis test.

①由环磷酸胺引起的小鼠造血系统抑制模型；②小鼠碳粒廓清试验；③小鼠溶血素形成试验；④小鼠游泳试验；⑤小鼠耐缺氧试验；⑥小鼠旷野活动试验；⑦由戊巴比妥钠引起的小鼠催眠模型。

This may partially attribute to packaging an inclusion within the hydrophobic exosporium so as to keep the inclusion in the water where target larvae maybe actively feeding.

可能是因为芽胞在昆虫肠道内再次萌发，产生了其他致病因子，如肠毒素、溶血素等，与杀虫晶体蛋白协同作用；也可能是因为伴胞晶体受到疏水的芽胞外壁层保护而能在水中等一般伴胞晶体不容易存在的地方被易感昆虫摄食。

Results We found the result of the samples are mostly aroused by hemolysis or feculent serum and the way of eliminating the fake result is the different way of cleaning the troughs.

结果 引起假阳性结果主要来源于有溶血或浑浊的血清，而消除其假阳性结果的方法主要在于不同的洗板方式。

Objective To study the influence of HBsAg results with ELISA of different cleaning the troughs Methods The hemolysis or feculent serum samples is frequent in the ordinary work.The HBsAg test by ELISA of the samples are often present fake positive.

目的 探讨不同洗板方式的酶联免疫法对HBsAg结果的影响方法在日常工作中，伴有溶血或其它因素引起浑浊的血清样品较常见，而些样品在HBsAg的ELISA检测中常出现假阳性，为消除这些假性结果。

ResultsCompared with model group, the clearance rate K of charcoal particles, phagocytic index a, thymus and spleen index and ear swelling degree of 80 mg/kg.bw Fortunella crassifolia flavonoid dose group were significantly improved(P.05), and a very significant effect (P.01) was made to the HC50 value at this dose.

结果与模型对照组相比，金橘黄酮80 mg/kg·bw 剂量时，小鼠碳粒廓清指数K、吞噬指数a、胸腺和脾脏指数、耳肿胀度有显著提高（P0.05），血清溶血值（HC50）有极显著提高（P.01）；金橘黄酮160 mg/kg·bw 剂量对全部试验参数的影响具有极显著性（P.01）。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。