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In the current study, a series of cationic α-helical peptides of 20 a.a. were designed and synthesized based on four structural parameters, including charge, polar angle, hydrophobicity, and hydrophobic moment. Several methods were performed to describe the influence of these parameters on the antimicrobial and hemolytic activity, and on the permeabilizing effect on model membranes. The potential of these parameters to increase the antibacterial activity and to improve the prokaryotic selectivity is assessed.

本实验利用自行设计合成的人工抗菌胜肽当模型,固定长度为 20 个胺基酸,改变其电荷、极性角度、疏水性及疏水性矩等四种结构特性,并利用其对各种不同病原菌之抗菌活性及对兔子红血球细胞的溶血活性来研究其结构特性的改变对其抗菌活性、选择性及微脂体膜的渗透性之影响,以期望改变结构参数能增加抗菌活性及对原核的选择性。

For a peptide to act as part of the host defense system or to be of pharmaceutical interest, high antimicrobial activity must be combined by low hemolytic activity. Our results indicated that the antimicrobial activity increased with increasing charge and polar angle, with charge of +4 and polar angle of 140° exhibiting the highest antibacterial and membrane permeabilizing activity. Higher hydrophobicity and hydrophobic moment correlated with increased hemolytic activity, thus decreasing selectivity dramatically.

胜肽要扮演宿主免疫机制的一部分或要成为新型态的抗生素不仅要有强大的抗菌活性还需要有高度的选择性,由本实验的结果发现,当抗菌胜肽的正电荷及极性角度增加时,胜肽之抗菌活性会随之增加,当抗菌胜肽的电荷为+4而极性角度为 140°时,胜肽有最强之抗菌能力及膜渗透活性;而随著胜肽疏水性以及疏水性矩的增加,会增加溶血活性,结果反而降低了抗菌胜肽的选择性。

Abstract] Objective: To investigate the effect of polysacchaide of silkworm pupa on immunological function in mice.

目的:通过测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响,研究蚕蛹多糖对小鼠免疫功能的影响。

Objective: To investigate the effect of polysacchaide of silkworm pupa on immunological function in mice.

目的:通过测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响,研究蚕蛹多糖对小鼠免疫功能的影响。

In another experiment, the addition of AGE to a rat erythrocyte suspension reduced the rate of peroxynitrite-induced hemolysis in a concentration-dependent manner, suggesting that AGE protects erythrocytes from membrane damage induced by peroxinitrite.

在另一个试验中,AGE加入大鼠红细胞悬液中可以以浓度依赖的方式减少过亚硝酸盐诱导的溶血速率,提示AGE可以保护红细胞膜免受过亚硝酸盐损坏。

Objective:To explore the relationship between the valence of antibody of pregnant woman in serum and Hemolytic Disease of Newborn.

目的 探讨夫妇血型、孕妇血型抗体筛选及抗体效价与新生儿溶血病之间的关系。

Third trimester of pregnancy, amniotic fluid aspiration can be measured to check blood type, bilirubin, lecithin, sphingomyelin, placental lactogen and so on, to understand whether or not the mother-fetal blood group incompatibility, hemolysis, fetal lung maturity and skin maturity, placental function.

妊娠晚期,羊水穿刺检查可测定血型、胆红素、卵磷脂、鞘磷脂、胎盘泌乳素等,了解有无母儿血型不合、溶血、胎儿肺成熟度、皮肤成熟度、胎盘功能等。

Background— Numerous in itro studies suggest that sphingosine 1-phosphate (S1P), a bioactie lysosphingolipid associated with high-density lipoproteins, accounts at least partly for the potent antiinflammatory properties of high-density lipoprotein and, thereby, contributes to the antiatherogenic potential attributed to high-density lipoproteins.

背景——众多的活体研究表明鞘氨醇1-磷酸盐作为一种与高密度脂蛋白相关的生物活性溶血磷脂,至少是高密度脂蛋白有力抗炎特质的部分原因,从而促成高密度脂蛋白的抗动脉粥样硬化潜能。

Dr. Philip Levine and Dr. Rufus Stetson publish a paper showing that baby-mother incompatibility is the cause of hemolytic disease of the newborn.

菲利普列文博士和鲁弗斯斯泰特森博士发表了一篇论文,婴儿和母亲的血液不相容是新生儿溶血病的起因。

In 1939 Philip Levine and Rufus E. Stetson published their findings about a family who had a stillborn baby who died of hemolytic disease of the newborn.

1939年,菲利普列文博士和鲁弗斯E斯泰特森博士发表了他们的一项发现,该发现是关于一个生产了死胎的家庭,该胎儿死于新生婴儿溶血病。

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