溶血

Glucose-6-phosphate dehydrogenase (G6PD, E. C. 1. 1. 1. 49) is a house-keeping enzyme in all cells, and also is a key enzyme in the pentose phosphate pathway. G6PD deficiency, affecting 200～400 million people in the world, can cause chronic non-spherocytic hemolytic anemia, favism, drug-induced hemolytic anemia, neonatal jaundice and several infectious diseases. Its inheritance mode is an X-linked incomplete dominance.

葡萄糖-6-磷酸脱氢酶（Glucose-6-Phosphate Dehydrogenase，G6PD，E.C.1.1.1.49）是存在于所有细胞中的一种看家酶，也是磷酸戊糖途径的关键酶。G6PD缺乏症累及全球2～4亿人，引起慢性非球形细胞溶血性贫血、蚕豆病、药物性溶血、新生儿黄疸及某些感染性溶血，其遗传方式是X连锁不完全显性方式。

A numerical method to estimate hemolysis level in blood pump was presented and hemolysis indices were calculated.

给出了一种利用数值方法计算溶血指标的方法，并通过溶血指标的计算评价了血泵内的溶血程度，发现雷诺切应力是导致溶血的主要原因。

Hemolyzed group was constructed from non-hemolyzed samples using mechanical methods. The Wilcoxon Signed Rank test was applied for testing the statistical significant difference and the Bland-Altman analysis was used for clinically importance evaluation.

本研究采用放射免疫分析法ELSA-CA 125 Ⅱ同时进行溶血与无溶血检体之血清CA 125抗原浓度测定，再将结果以统计方法分析，评估溶血检体是否会干扰CA 125的测定。

But we have seldom found the reports about it in China; So writer summarized some literature more than twenty years; name,classification and family of hemolysin have been written, especially three of representative species:α-toxin, Streptolysin O and HlyA; Nowaday, A relationship between the hemolysin and pathopoiesis of bacterium is obvious.

另外，细菌溶血素的英文命名并不统一，检索时容易造成漏检或误检；通过查阅大量英文文献资料，归纳汇总近20年报道的溶血素研究进程；该文详细列出了细菌溶血素名称、分类以及各溶血素家族列表。并且分别介绍了其中三种典型的穿孔毒素：葡萄球菌α-毒素、链球菌溶血素-O、埃希氏大肠杆菌溶血素HlyA。

Methods: Picric performance rate method is used to measure anhemolytic samples, factitious hemolytic samples, post-factitious hemolytic samples with 0.88mol/L H2O2 37℃ water bath for 20min, post-factitious hemolytic samples with 0.88mol/L H2O2 37℃ water bath for 30min, anhemolytic samples with 0.88mol/L H2O2 37℃ water bath for 20min to deal with five of the numeral value of creatinine in blood samples.

用苦味酸速率法测定未溶血、人为溶血、人为溶血后加0.88mol/L H2O237℃水浴20m in、人为溶血后加0.88mol/LH2O2 37℃水浴30min、未溶血加0.88mol/L H2O2 37℃水浴20m in五种处理的血液样品中肌酐值。

Results: None of 12 recipients developed acute transfusion related hemolysis during infusion of stem cells. Both major and minor ABO incompatibility had no adverse effects on engraftment and recovery of platelets. However, the major ABO-incompatible group showed trends of slower erythropoietic recovery and 1 recipient in this group experienced delayed hemolysis.

结果：12例ABO血型不合的allo-HSCT受者在输入造血干细胞悬液时无1例发生急性溶血和输血相关性溶血，主要不合和次要不合对allo-HSCT骨髓植活和血小板恢复均无影响，但主要不合组红系恢复有延迟的趋势，有1例发生迟发性溶血。

Methods:The fresh porcine blood added sodium citrate was used for extracting of heme. The methods of hematolysis by water,ethanol and supersonic wave were compared.

新鲜抗凝猪血为原料，比较了水溶胀法、乙醇法以及超声波法对红细胞溶血效果，以盐酸丙酮配比，盐酸丙酮与溶血液体积比对猪血中血红素提取的影响。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因，即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联，得到三联融合毒素基因tdh-vvhA-vmhA。

The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

A VHH probe, which is specific to V. harveyi vhhA haemolysin gene, was used to screen EcoR Ⅰ digests of total DNA from 57 vibrio strains, including 26 vibrio type strains, 20 V.

哈维氏弧菌的VHH溶血素基因与副溶血弧菌(V.parahaemolyticus)的TLH热不稳定性溶血素基因具有高度相似性，其氨基酸序列的相似性达到85.6﹪。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。