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There were many reasons attributed to this phenomenon: macromolecules, which were from raw material such as polysaccharides and proteins decomposed incompletely in the process, formed precipitation through biochemical reaction, many kinds of bacteria were not removed totally after fermenting, which caused bacterial precipitation, Fe3+ from fermenting equipments combined with polyphenol and proteins to form infusibility complex.

中文摘 要:食醋在其保质期内经常出现混浊现象,其原因主要有:原料中的大分子物质,如多糖、蛋白质等分解不彻底,后经过生化反应形成沉淀物质;发酵完成后,食醋中的各种微生物未及时清除,从而造成的菌体性沉淀;以及由于生产设备中的Fe3+与原料中的多酚、蛋白质等大分子形成的不溶性络合物所造成的沉淀。

The fermentation characteristics of W39 is as follows: it can yield in the liquid the insoluble gel-like cellulose only with the existence of oxygen. The forming of nata needs uncovered surface of static liquid.

研究了该菌在椰子水培养基上的发酵特性:经液态发酵产生不溶性的凝胶纤维素,且只在有氧生长时才合成纤维素;对无机盐和生长因子的要求复杂;用于纳塔生产时必须静置培养并保证液面与空气接触。

Through researching the mechanism of biological nitrification and denitrification and actual project application of the technology and related literature materials, the obtained results are as follows:(1) Anoxybiosis is effective to the removal of COD; after the anoxic process, BOD/COD of the outflow increases, it shows that the degradation ability of wastewater is improved; anoxybiosis is insensitive when shock loading of inflow wastewater varies greatly;(2) It is demonstrated by the operation of inoculation and cultivating that biofilm takes two weeks to succeed in cultivating on packings and the removal ratios of NH3-N and COD get 50% and 70% respectively, when gas-water ratio is 6:1, HRT is 7.3 hour, water temperature is above 15°C;(3) It is demonstrated that gas-water ratio, hydraulic loading, HRT, temperature, pH etc are important factors which influence the removal effect of pollutants, when the gas-water ratio is 6:1, hydraulic loading is about 0.38kgNH3-N/m~3.d, water temperature ranges from 15°C to 27°C, pH ranges from 7.5 to 8.0, the BIOFOR removal effect of pollutants is the best;(4) The operation indicated that, this technology has strong ability of nitrification and denitrification; it has extremely vital significance for reducing the water body eutrophication;(5) It is confirmed the feasibility and the usability of preanoxic-BIOFOR process to treat L-lactic acid production wastewater. The experiment indicated that the treatment effect of this technology is very good and also shows that the technology has many advantages, such as small volume, high treatment efficiency, good effluent quality and strong endurance to load variation, this technology is a new and economical wastewater treatment process.

通过对生物脱氮机理的研究和该组合技术的实际工程应用,并结合有关文献资料,取得如下结论:(1)预缺氧池对L-乳酸生产废水中的COD去除具有一定的效果;废水中难降解的高分子物质经过缺氧工段分解为易于氧化降解的物质,浮状有机物在水解菌的作用下,将不溶性有机物水解为溶解性物质;预缺氧池具有较强的抗冲击负荷能力;(2)BIOFOR接种挂膜过程表明:HRT为7.3h、气水比为6:1、水温保持在15℃以上,经过半个月生物膜即以形成,NH_3-N和COD的去除率分别达到50%和70%左右;(3)研究表明:气水比、水力负荷、HRT、温度、pH等是影响污染物去除效率的重要因素,在气水比为6:1,NH_3-N容积负荷为0.38kgNH_3-N/m~3·d,温度在15℃-27℃,pH在7.5-8.0之间的条件下,BIOFOR对污染物的去除效率达到最佳;(4)运行表明,该组合技术具有较强的脱氮能力,对于减少水体富营养化具有十分重要的意义;(5)经过半年多实际工程的现场调试与运行,验证了利用预缺氧—BIOFOR组合工艺处理L—乳酸生产废水的可行性和实用性,得出该组合技术具有占地面积小,处理效果好,运行费用省,耐冲击负荷能力强等优点,是一项新型、经济的污水处理技术。

T standard strains and one fermentative strain MZ1 as templates, PCR results showed that 16 strains, which may be lysogenic strains, produced the product with the size about 750bp as anticipated.

t标准菌株和生产菌株MZ1(血清型H3a3h)的过夜培养物作模板,结果有16株菌株包括MZ1能扩增出大小约为750bp的预期产物,推测这16株菌为溶原菌。

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