溶菌生产的

And European countries. Bacteriolytic enzyme can be used to decompose cell wall and has a wide application. The amount presently produced in the country can only meet the one third of market demand and mainly sold to Dalian and Shanghai.

溶菌酶由于具有专一性分解细胞壁的能力，用途十分广泛，目前国内生产的该产品只能满足市场需求的三分之一，产品主销大连、上海。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

According to one conserved sequences of phage GIL01 from Bacillus thuringiensis,a pair of primers was designed.With overnight cultivations of 35 B.t standard strains and one fermentative strain MZ1 as templates,PCR results showed that 16 strains,which may be lysogenic strains,produced the product with the size about 750 bp as anticipated.

根据苏云金杆菌(Bacillus thuringiensis， B.t)溶原性噬菌体GIL01(GenBank Accession Number： AJ536703)的同源序列设计一对引物，用35株B.t标准菌株和生产菌株MZ1(血清型H3a3b)的过夜培养物作模板，结果有16株菌株包括MZ1能扩增出大小约为750 bp的预期产物，推测这16株菌为溶原菌。

Among them strain MZ1 had been confirmed a lysogenic strain by indicator strain ZK1 after producing phage plaques induced by Mitomycin C.Two templates of overnight cultivation and its DNA from strain MZ1 were then amplified in the same time with five pairs of primers designed from the same phage,and the same products were obtained,suggesting that strain MZ1 was also confirmed a lysogenic strain by PCR method.

MZ1菌株的过夜培养液与指示菌ZK1(血清型H25)混匀并在双层琼脂上培养12 h后出现了滴度为2×107 pfu的噬菌斑，从而证明生产菌株MZ1确为溶原菌；又以GIL01同源性较高的其它四个基因设计引物，分别以MZ1的培养物及其溶原性噬菌体基因组为模板进行PCR扩增，结果两者得到几乎一致的带型，进一步证明了生产菌株MZ1为溶原菌，说明利用PCR法检测鉴定苏云金杆菌的溶原性是可行的。

Based on the investigation of the transesterification between dimethyl sebacate and 1,2,2,6,6-pentamethyl-4-piperinol, the synthetic technologies of Tinuvin292 and Tinuvin 765 were established. The two kinds of hindered amine light stabilizers were obtained in the yield of 93.3% and 92.4% respectively and qualified as the advanced level of the kindred overseas products.2. The synthetic technology of Tinuvin123 was studied and established. Using 2,2,6,6-tetramethyl-4-piperinol as the starting material, the product was synthesized successfully through oxidation, free-radical reaction and transesterification in overall yield of 57.6%.

本文研究建立了三个受阻胺类光稳定剂的合成工艺，探索了聚合型受阻胺类光稳定剂生产过程中不溶性副产物产生的原因，并设计、合成了一系列具有抑菌活性的新型受阻胺类光稳定剂。1、在研究癸二酸二甲酯与1，2，2，6，6-五甲基哌啶醇的酯交换反应的基础上，建立了受阻胺类光稳定剂Tinuvin292、Tinuvin765的合成工艺，分别以93.3%和92.4%的收率得到了光稳定剂Tinuvin292、Tinuvin765，所得产品质量达到了国外同类产品的水平。2、研究建立了受阻胺类光稳定剂Tinuvin123的合成工艺。

The synthetic technologies of three hindered amine light stabilizers were established, the reason for the insoluble side products formed in the production of polymerized hindered amine light stabilizers were studied, and a series of novel hindered amine light stabilizers with antibacterial properties were designed and synthesized in this present paper.1. Based on the investigation of the transesterification between dimethyl sebacate and 1,2,2,6,6-pentamethyl-4-piperinol, the synthetic technologies of Tinuvin292 and Tinuvin 765 were established. The two kinds of hindered amine light stabilizers were obtained in the yield of 93.3% and 92.4% respectively and qualified as the advanced level of the kindred overseas products.2. The synthetic technology of Tinuvin123 was studied and established.

本文研究建立了三个受阻胺类光稳定剂的合成工艺，探索了聚合型受阻胺类光稳定剂生产过程中不溶性副产物产生的原因，并设计、合成了一系列具有抑菌活性的新型受阻胺类光稳定剂。1、在研究癸二酸二甲酯与1，2，2，6，6-五甲基哌啶醇的酯交换反应的基础上，建立了受阻胺类光稳定剂Tinuvin292、Tinuvin765的合成工艺，分别以93.3%和92.4%的收率得到了光稳定剂Tinuvin292、Tinuvin765，所得产品质量达到了国外同类产品的水平。2、研究建立了受阻胺类光稳定剂Tinuvin123的合成工艺。

To improve spinosyn-producing strain and enhance spinosyns yield, we studied the effects of glycin concentration and the operational time, temperature and lysozyme concentration on protoplast preparation of Saccharopolyspora spinosa SP06081. We also studied different regeneration media and osmotic stabilizing agents.

为了改良多杀菌素生产菌种，提高多杀菌素产量，研究了甘氨酸添加浓度、溶菌酶作用时间、温度和浓度对多杀菌素生产菌刺糖多胞菌Saccharopolyspora spinosa SP06081菌株原生质体制备和再生的影响，并考察了不同再生培养基和渗透压稳定剂对其再生的影响，确定了该菌株原生质体制备和再生的最佳条件。

With cerevisiae waste yeast as key raw material, we introduce one way to produce shoyu by adding antalzyme to dissolve yeast and malt rootlets enzyme to degrade RNA. We got this on the basis of experiments.

在实验基础上提出了以啤酒废酵母为主要原料，添加溶菌酶与麦芽根酶进行酵母溶解与RNA的降解等工艺方法生产酱油的途径。

A series of IImethods, including addtion of liquid petrolatum and glass beads and adjustment offlask loads and rotation speed of swing bed, was founded to be efficient in controllingthe concentration of dissolved oxygen in fermentation medium and enhancing thetransmission of substances.

通过添加液体石蜡封层、调节转速、装液量和添加玻璃珠的一整套手段有效地控制了发酵液的溶氧量，提高了传质效果，从而促进了菌体的生长和D-乳酸的生产。

In the past, researches focused only on sole function of bacteria such as tricalcium phosphate or ferric phosphate solubilization. In order to efficiently develop superior phosphate solubilizing microorganisms which are useful on various crops, a team includes Professors C.C. Young, C.C. Tang, S.H. Su, H.J. Chen and C.T. Lo in National Chung Hsing University and National Formosa University was established. Microbial consortiums include phosphate solubilizing bacteria, phosphate solubilizing actinomycetes, phosphate solubilizing yeast, and phosphate solubilizing fungi will be designed in the present study and their capabilities to promote crop growth will be demonstrated. The suitable phosphate solubilizing bacterial formula will also be designed. Consideration for the specificity and adaptation of phosphate solubilizing microorganisms to their host crops, the designed biofertilizers will be used as inoculants to increase the yields of various crops meanwhile reduce the overdose usage of chemical fertilizers.

过去研究溶磷菌主要是在各别溶磷之功能上之研发，如溶磷酸钙及溶磷铁，本计画为缩短不同菌种筛选在不同作物之效应评估工作，以组成一研究团队进行不同类型菌种之应用评估，成员涵盖二个大学的研究团队，包括杨秋忠、谭镇中、苏孝华及罗朝村等教授，分别研究细菌、放线菌、酵母菌及木霉真菌，另在菌剂生产配方由陈鸿基教授研究，以提高多元化应用土壤磷素之有效利用，并达到不同作物之应用效应评估，期以探讨开发不同作物专用之微生物肥料之可行性及问题，以减少化学磷肥之施用，降低土壤劣化提高农民所得之目的。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。