溴化氰

Purpose To study the effect of different factors on the efficiency of cleaving fusion protein with cyanogen bromide.

目的 研究不同因素对溴化氰切割融合蛋白的影响。

METHODS: Affinity column constituted by gelatin coupling with sepharose 4B activated with cyanogen bromide was used to purify fibronectin.

用明胶与溴化氰活化琼脂糖珠偶联的亲和层析柱对纤维蛋白结合素进行纯化。

Methods Activate GAMP with cyanogens bromide and prepare GAMP-protein conjugates using ADH as a linker and EDAC as a coupling agent.

方法采用溴化氰活化多糖，以无水己二酸二肼为连接剂，1-乙基-1-3-(3-二甲基氨基-丙基)-碳化二亚胺为偶联剂，制备结合物并免疫小鼠，检测小鼠血清中抗GAMP与抗载体蛋白抗体及抗GAMP抗体的杀菌活性。

Methods GAMP was activated with cyanogen bromide and combined with 1, 6-adipic acid dihydrazide. The prepared polysaccharide derivative GAMP-ADH was coupled to recombinant protein Pep10 with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride.

GAMP经溴化氰活化后，共价接合己二酰肼手臂，在碳二亚胺催化下与重组蛋白Pep10偶联，制备结合物GAMP-ADH-Pep10。

Methods Activate GAMP with cyanogens bromide and prepare GAMP-protein conjugates using ADH as a linker and EDAC as a coupling agent. Immunize mice with the conjugates and determine the antibodies against GAMP and carrier proteins in their sera as well as the bactericidal activity of antibody against GAMP.

采用溴化氰活化多糖，以无水己二酸二肼为连接剂，1-乙基－1-3-（3-二甲基氨基－丙基）－碳化二亚胺为偶联剂，制备结合物并免疫小鼠，检测小鼠血清中抗GAMP与抗载体蛋白抗体及抗GAMP抗体的杀菌活性。

We successfully improved the methods of departing O-methyl using boron tribromide, of isolating hydrogen bromide byα-chloro-ethyl chloroformate, and of related in synthesis of natrexone, new medicine, using boron tribromide. This resulted in generation of hydrogen cyanide becoming impossible.

改进了以氯甲酸-α-氯乙酯脱代替溴化氰以及改进的三溴化硼法作为新药纳曲酮合成中相应的方法，去除了合成过程中产生氰化氢气体的可能性。

Because of no signal from N-terminal protein sequencing for 43kDa protein directly, a resulting 18kDa peptide from the partial cleavage of 43kDa protein by BrCN had its N-terminal sequences of AFTFKK determined by N-terminal protein sequencing assay. Then, the 43kDa protein was convinced to the enzyme of 3phosphate glycerate kinase (PGK1) by searching YPD.

由于43kDa蛋白直接进行蛋白质氨基端序列测定时没有信号，因而用溴化氰部分化学裂解43kDa蛋白，得到的18 kDa多肽经蛋白质氨基端序列测定，得到其氨基端前6个氨基酸残基序列为AFTFKK，通过计算机检索YPD，确定43 kDa蛋白是3—磷酸甘油酸激酶（PGK1）。

The present invention uses isocyanuric acid as main raw material, and adopts the following main steps: adding alkali to make solvation, chlorization, bromation, secondary chlorization, filtering and drying to prepare bromochloro-isocyanuric acid.

本发明公开了一种溴氯异氰尿酸消毒灭菌剂的制备方法。该方法以异氰尿酸为主要原料，通过加碱溶解、氯化、溴化、再氯化、过滤、干燥主要步骤制备溴氯异氰尿酸。

Methods Activate groups A and C meningococcal polysaccharide with cyanogens bromide and prepare into MenAPS-ADH and MenCPS-ADH derivatives by reacting with 1, 6-adipic acid dihydrazine. Mix MenAPS-ADH and MenCPS-ADH derivatives with purified tetanus toxoid at a certain mass ratio, then add l-ethyl-3(3-dimethylaminopropyl)-carbodiimide to prepare bulks of MenAPS-TT and MenCPS-TT conjugates respectively. Mix the two bulks at a certain ratio, add stabilizer and lyophilize to prepare group A+C meningococcal polysaccharide conjugate vaccine.

A群和C群脑膜炎球菌多糖在溴化氰的活化下，以1，6己二酰肼作为连接子，与精制破伤风类毒素在碳二亚胺作用下结合，再按一定的比例将两者混合，制成A+C群脑膜炎球菌多糖结合疫苗原液，加保护剂冻干后，制成A+C群脑膜炎球菌多糖结合疫苗。

We verified the purification procedure by SDS-PAGE and Western blot. Their interactions were investigated by using Far-Western blot and Pull-down assay.

通过葡萄球菌V8蛋白酶和溴化氰对P4.2蛋白的限制性酶切分析，我们选取了一系列序列重叠的P4.2片段进行克隆表达。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新