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The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变；沉淀回收的PCR产物以20μl变性上样液（95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝）溶解后热变性为两条DNA单链后，上样于8%非变性聚丙烯酰胺凝胶（含5%甘油），700伏电压、4℃电泳16小时。

METHODS Acidic dye colorimetry with bromothymol blue as ion pair reagent was employed to determine the alkaloids in samples.

用溴麝香草酚蓝同川芎生物碱形成稳定的有色离子对酸性染料比色法测定总生物碱的含量。

The Teflon cell contains a pH sensitive indicator-buffer solution consisting of bromothymol blue and sodium carbonate.

更多作为C02选择透过性膜，该液芯波导管同时也是传感器的比色池，我们采用溴百里酚蓝和碳酸钠(Na2CO3)溶液为主要成份的pH敏感指示剂作为缓冲溶液。

With sodium succinate as carbon source and bromothymol blue as pH indicator,20 BTB agar plate positive strains were isolated,from which four of them were identified as aerobic denitrifiers.

利用琥珀酸钠作为碳源，溴百里酚蓝作为pH指示剂，共筛选得到20株BTB琼脂平板阳性菌。通过反硝化性能测定，复筛得到4株好氧反硝化细菌。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程，我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理，建立了一种简捷的比色法，用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂，在495 nm和615 nm处检测二者的光吸收值的变化，可以测定酶活。本方法的优点是，可在比较宽的酶浓度范围内进行活力测定，而且操作简单、节省样品、灵敏度高。

The proposed method involves forming coloured ion-pair from ephedrine hydrochloride and bromothymol blue at pH 6.5 extracting the ion -pair thus into chloroform and measuring the absorbance chloroform solution at 414 nm.

根据盐酸麻黄碱在一定pH条件下，可与溴麝香草酚蓝反应生成稳定的络合物这一特性，选择414nm波长处测定其含量。

The conclution is confiemed mutually by the tested result of TBAB distribution coefficients in the differen phases in Benzene/TBAB/water/KBr system,the distribution coefficients is tested by the method of Bromothymol Blue Spectrophotometric Determination.

得到结论同溴百里酚蓝分光光度法测定苯/TBAB/水/KBr体系各相态中TBAB分配系数的结果相互验证。

Methods:total alkaloids of radix Sophora lavescens were extracted by acidified water,then adjusted to neutral by sodium hydroxide solution, dissolved with buffer solution,then were mixed with 0.025％ of bromothymol solution and chloroform,and then the mixture was shaken,standing and demixed,the absorbability of chloroform layer was detected at the wavelength of 413nm.

将苦参的酸水提取液用氢氧化钠溶液调节至中性后，依次加入pH=7.6缓冲溶液，溴代麝香草酚蓝指示液、氯仿混合振摇，静置分层，于413 nm波长处测定氯仿层吸收度。

Surface seawater samples are pumped into a PVC tube enclosing an inner Teflon AF tube, which served as a long pathlength gas-permeable liquid-core waveguide for spectrophotometry. The Teflon cell contains a pH sensitive indicator-buffer solution consisting of bromothymol blue and sodium carbonate.

该传感器采用Teflon AF 2400液芯波导管作为C02选择透过性膜，该液芯波导管同时也是传感器的比色池，我们采用溴百里酚蓝和碳酸钠(Na_2CO_3)溶液为主要成份的pH敏感指示剂作为缓冲溶液。

Serum alt,ast,and bile acid were observed by enzyme assay and the ratio of ast/alt was worked out. serum bilirubin was measured by peariman and lee method. serum albumin was tested by bromphenol assay and was simultaneously measured to hyaluronic acid, type ⅲ procollagen, laminin and type ⅳ collagen by ria. meanwhile, prothrombin time and platelet count were measured and so on. results: the level of serum aca was raised increasingly in different cirrhotic cases with different classifications of child-pugh, according to severity of the condition.

应用酶联免疫吸附法分别对不同分级的肝硬化患者血清中抗心磷脂抗体的含量进行了测定；用酶法测定了alt、ast与胆汁酸并计算ast/alt值；用peariman和lee改良法测定血清胆红素；用溴甲酚氯比色法测定血清白蛋白；用放射免疫分析法测定透明质酸、ⅲ型前胶原氮端肽、层粘连蛋白与ⅳ型胶原；并测定了凝血酶原时间与血小板计数等相关指标。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新