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The inhibitory effect of nickel to the reaction of H_2O_2 oxidizes bromocresol purple had been researched in the buffering medium of pH=8~9 and the method of inhibitory kinetic spectrophotometry for determining nickel had been set up.

发现了在 p H=8～ 9的缓冲介质中，Ni2 +对 H2 O2 氧化溴甲酚紫的反应有明显的阻抑作用。

Methods Acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.

目的 建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法，以溴甲酚绿缓冲液作为生物碱的显色剂，测定样品溶液在417 nm波长处的吸收度。

The method is based on the oxidation of bromocresol green with potassium periodate using manganese as a catalyst and nitrilo triacetic acid as an activator in a solution of HAc-NaAc.

研究了在HAc-NaAc介质中，以氨三乙酸为活化剂，锰离子催化高碘酸钾氧化溴甲酚绿的褪色反应，建立了测定痕量锰的新方法。

The method is based on the cobalt catalyzing oxidation of bromocresol purple by hydrogen peroxide in the media of ammonia water using potassium chloride as an activator.

研究了在氨水溶液介质中，以氯化钾溶液为活化剂，痕量钴离子催化过氧化氢氧化溴甲酚紫褪色的指示反应，通过测量590 nm处溶液吸光度的改变，建立了催化动力学光度法测定痕量钴的新方法。

The optimal measuring conditions were investigated. The results indicated that the catalytic reaction to Pt and bromocresol purple was the first-order reaction. The apparent activation energy was 217.4kJ/mol and the maximum absorption wavelength of fading reaction was at 434 nm. The linear range of Pt was 0.16-4.0μg/L and the detection limit was 2.5×10^(-11)g/mL. Most of common ions had no interference with the determination, while ten multiples of Ru, Rh and Os had interference with the determination of Pt. Therefore.

选择了最佳测定条件，实验结果表明，催化反应对铂和溴甲酚紫为一级反应，反应的表观活化能为217.4kJ/mol，褪色反应的最大吸收波长为434nm，方法测定R哪的线性范围为0.16~4.0μg／L，检出限为2.5×10^(-11)g/mL，大多数常见离子不干扰测定，10倍以上的Ru，Rh和Os对测定有干扰，可在测定前分离除去。

At the same time, using bromocresol green - methyl red indicator and phenolphthalein indicator control instructions with the free acid end of the titration end sensitive and accurate.

同时，采用溴甲酚绿-甲基红指示剂与酚酞指示剂配合指示调控游离酸的终点，使滴定终点灵敏、结果准确。

A new method was developed for detection of the hydroxyl radical in Fenton reaction by bromocresol green oxidative assay. After oxidized by hydroxyl radical, the absorbance of bromocresol green is decreased apparently.

利用Fenton反应产生的羟基自由基与染料试剂溴甲酚绿(Bromocresol green， BG)发生氧化反应后使其吸光度值降低，建立了一种可间接测定羟基自由基的新方法。

Methods:Radioimmunoassay method was used to detect the levels of IL-6 in the cerebral spinal fluid.nephelometry method was used to detect the levels of albumin in the CSF and bromocresol green method was detect the levels of albumin in the serum.

用放射免疫法检测IL-6、溴甲酚绿法检测血清白蛋白、免疫比浊法检测脑脊液白蛋白。

Guangdong college of pharmacy,guangzhou,guangdong 510006,china;henan university of traditional chinese medicine,zhengzhou,henan 450008,chinaabstract:objective to establish a method for the determination of the content of total alkaloid in nao mai tong recipe.methods acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.results the calibration curve of the absorbance was linear with the concentration of ligustrazine in the range of 0.031～0.153 mg/ml(r=0.9998).the average recovery of ligustrazine was 100.95％,rsd=1.75%.

作者单位：广东药学院中药化学与分析教研室，广东广州 510006；河南中医学院，河南郑州 450008 目的建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法，以溴甲酚绿缓冲液作为生物碱的显色剂，测定样品溶液在417 nm波长处的吸收度。结果对照品川芎嗪质量浓度在0.031～0.153 mg/ml的范围内与吸光度呈良好的线性关系，r=0.9998。平均回收率为100.95％，rsd为1.75％。精密度和稳定性良好。

MethodsThe content was determined by solvent extraction and acid dye colorimetiy, with Acanthoine Ruscope-ine （C16H22N4O2） as a control criterion and bromocresol green as the acid dye, and the wavelength was at 418 nm.

方法采用溶剂萃取酸性染料比色法测定，以去氢飞廉碱（C16H22N4O2）为对照品，溴甲酚绿为酸性染料，用氯仿萃取，检测波长418 nm。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。