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Methods The experimental TBI model was established by bumpiness of free falling body according to Feeney′s.The rats′cerebral edema were imaged with magnetic resonance image,The changes of brain water content and permeability of blood-brain barrier were measured by the methods of wet and dry weight and Evans blue fluorometry.The expression of AQP-4 mRNA was examined by reverse transcriptase polymerase chain reaction.

按照改进的Feeney自由落体撞击法建立大鼠创伤性脑损伤模型,用磁共振成像对大鼠脑水肿进行检测,干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时相脑组织含水量和BBB通透性的变化,并采用逆转录聚合酶链反应法检测AQP-4 mRNA的表达,蛋白质免疫印迹法检测AQP-4蛋白的表达。

Joes sleep system-silk quilt series uses the latest high technology to remove fat increasing silk's softness. Combining suitable high quality fabric with exquisite sewing technology perfectly embodies silk quilt's softness, breathability, humidity-repellence, comfort and warmness, etc like newborn baby's skin, protecting your health ever and again.

乔德睡眠系统的蚕丝被系列,采用最新的高科技术进行脱脂处理,提高蚕丝胎松软度,不易饼结,选用更合适的高品质外套,结合精湛的缝制工艺,使得蚕丝被德轻柔性、透气性、排湿性、舒适性及保暖性等特质得以完美体现,犹如新生婴儿的肌肤般柔软,时时呵护着您的健康。

Sericultural for actin fibers, fine silk and the tenacity of porous, high thermal performance, are cotton, 1.2 times the hygroscopicity of cotton are 1.5 times the elastic flexibilityìcompressed cotton are 3 times 2 times the wool to prevent cold bones moisture penetration, and exchange of steam.

蚕丝为动蛋白纤维,丝细多孔而坚韧、保暖性能极高,是棉花的1.2倍,吸湿性是棉花的1.5倍,压缩伸拉弹性弹性是棉花的3倍,羊毛的2倍,可防止寒湿气透入筋骨,并交换蒸汽。

By this method, the productivity of 3. 18g/l extracellular polysaccharides was obtained. The optimum extraction method was obtained by RSA. It was determined as follows: 91. 4 ℃, 2. 9h, the weight ratio between mycelia and water was 1 to 3. The productivity was 12. 38% of dry mycelia. Set the purifying methods of Grifola frondosa: Precipitated polysaccharides part by 60% ethanol→removed protein by Sevag method→removed coloring matter by H〓O〓→removed salts by dialyse→DEAE-cellulose column chromatogram→ purified polysaccharides groups.

对于胞内多糖的提取,采用湿菌体经捣碎再高压破壁的方式破碎菌体细胞,采用响应面分析法得出优化后的提取条件为:提取温度91.4℃,提取时间2.9h,料水比1:3,此提取条件下胞内粗多糖的率为干菌体重的12.38%建立了灰树花多糖分离纯化的技术路线 60%乙醇沉淀的粗多糖→Sevag法脱蛋白→H〓O〓脱色→透析脱盐→DEAE-纤维素层析分离→多糖组分。

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