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Ellagic acid helps reduce free radical damage and protect the nuclei of cell DNA against damage.

柔花酸能有效对抗游离基,更深层保护细胞中的DNA。

Objective To establish a method for the determination of 7 free fatty acids in human serum.

目的 建立人血清中7种游离脂肪酸的测定方法。

Abdominal adipose tissue is a source of free fatty acids and inflammatory factors.

腹部脂肪组织是游离脂肪酸和炎症因子的来源。

If there is any wound on skin, this dissociative fat acid would lead to turgescence and agnail and pain.

如果表皮有轻微破损,在游离脂肪酸的作用下会形成红肿发炎,同时形成痛感。

No obvious difference was observed when 6′-OH of isonucleoside was free or was protected by allyl group.

异核苷6′-OH处于游离和烯丙基保护状态时的结果没有显著差异。

However,the removal of soil noncrystalline aluminum with DCB soultioncaused an obvious decrease of soil SSA.3.The hydroxy aluminum in the interlayer of 1.4nm intergrade mineralhad an important negative effect on the cation exchange capacity ofsoil colloids.Its removal would cause a significant increase in CEC valuesof the soil colloids.4.Free aluminum was the major carrier of phosphate adsorption of soilcolloids.Particularly,the active aluminum had a decisive effect on thecapacity of phosphate adsorption of soil colliods.5.Addition to the effects of other soil constituents on soil surfaceproperties,it was thought that the effect of soil free aluminum,especially active aluminum on soil surface properties,deserved closeattention.

焦磷酸钠、草酸铵、DCB、柠檬酸钠和NaOH提取的土壤铝对提高土壤CECv、AEC、PZC和PZNC起重要作用,尤以活性铝的作用较大。2)用草酸铵处理除去铝后,土壤比表面积变化不大或略有增加;用DCB处理除去土壤铝后,土壤SSA明显降低。3)层间羟基铝对土壤胶体的阳离子交换量有显著负贡献,用柠檬酸钠将之除去后,土壤胶体的阳离子交换量明显增加。4)土壤胶体中的游离铝是土壤胶体吸附磷的主要载体,尤其是其中的活性铝,对土壤胶体的磷吸附能力起决定性作用。5)除其它土壤组分对土壤表面性质的影响外,土壤中的游离铝、尤其是活性铝对土壤有关表面性质的影响,值得引起特别的重视。

The experimental results show that the content of SiC in RBSC increases with carbon content while α-SiC is chosen as a filler. However, the residual carbon would appear in specimens when the carbon content exceeds 30% by weight. Using carbon felt, the reaction-formed SiC particles are small and uniform in size and distribution. However, when carbon felt is impregnated with phenol resin, the reaction-formed SiC is nonuniform in size and distribution. X-ray diffraction shows that the fabricated RBSC is composed of α-SiC,β-SiC, and free Si. The polytype of SiC is related to the reaction-heat during sintering.

结果表明:选用α-SiC+C粉的混合物作为生坯,SiC相的体积分数随生坯中wC的增加而增加,但过大的wC将使硅化后的试样出现残碳;选用碳毡作为生坯,反应烧结碳化硅的显微组织特点是C/Si反应生成的碳化硅颗粒均匀细小,并呈线状分布在游离硅中;浸渍过树脂的碳毡硅化处理后的显微组织特点是反应生成的碳化硅颗粒粗大且呈不均匀分布。X射线衍射结果也表明,反应烧结碳化硅陶瓷由游离Si、α-SiC、β-SiC组成,试样中不同晶型碳化硅的出现与C/Si反应的放热过程有关。

According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因,是残留标示物和蛋白偶联后的特征结构发生改变,诱导产生的抗体不能够对游离MQCA很好的识别,因此,本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构,并对其合成路线进行了筛选和优化,最终以对甲基邻硝基苯胺为起始原料,经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸,为制备对游离残留标示物高亲和力的抗体,高灵敏度的ELISA残留检测方法的建立奠定基础。

Experimental results showed that, in this process, the optimal pH of the shortcut nitrification was 8.0~8.5; when the pH was less than 6,the nitrification is completely inhibited; when the pH was 6~7, at the end of aeration, the Nitrobacteria become the dominant bacteria because of the dropping of the free ammonia concentration. It is known to all, the activity of the Nitrobacteria and Nitrosomonas was affected by FA concentration, so nitrification was affected by FA concentration. According to the author's research results, the FA concentration which inhibit the Nitrobacteria was below 1.0mg/L,which inhibit the Nitrosomonas was above 10mg/L.

实验研究结果表明:本工艺中,短程硝化反应的最佳pH值在8.0~8.5之间,当pH小于6时,整个硝化反应都会受到抑制,当pH在6~7之间时,随着游离氨浓度的降低,在曝气后期,硝化菌将会占据优势;FA浓度对短程硝化反应的影响是通过对亚硝酸菌和硝酸菌的抑制来实现的,在本研究条件下,游离氨对亚硝酸菌的抑制浓度在10mg/L以上,对硝酸菌的抑制浓度在1.0mg/L以下;温度对短程硝化反应的影响是双重的,既影响微生物的生理活性,又影响FA浓度。

The cutoff value of E2/E6, used to distinguish the pure episomal form from a mixed form of episomal and integrated HPV16 DNA, was 0.81 in the multiplex real-time PCR test. c) HPV16 existed as episomes in most of the CIN Ⅰ lesions, and 42.9% of them had mixed episomal/integrated HPV16. The integrated form of HPV16 constituted the majority in most of the CIN Ⅱand CIN Ⅲ cases. In squamous cervical carcinomas, the HPV16 was mostly integrated into the host chromosome. d) The rate of HPV16 integration was associated with the degree of cervical lesions and the rate increased with the progression of cervical disease, e) The rate of pure integrated HPV16 in stage Ⅱ and Ⅲ(88%) was higher than that in stage Ⅰ(33.3%). Conclusions: a) Multiplex real-time PCR is a rapid and sensitive method for detection of the integration state of HPV16 DNA. b) The integration of HPV16 DNA is a very early event in the development of cervical cancer and may act as an activation mechanism for progression from preinvasive to invasive cervical cancer, c) The pure integrated HPV16 in cervical cancer may be a useful prognostic indicator.

HPV—16E2、E6标准曲线相关系数均为1.00,扩增效率均在95%以上:2)确定0,81作为多重实时PCR区分游离型和混合型HPV-16的界值、3)CINⅠ中HPV—16大多以游离方式存在,但仍有整合的发生(42,9%);CINⅡ和CINⅢ中HPV—16以混合型为主:浸润癌中以整合型HPV—16为主。4)HPV-16整合发生率与不同程度的宫颈病变有关,并且随着宫颈病变级别的升高HPV-16整合发生率呈上升趋势-5)Ⅱ+Ⅲ期宫颈癌中整合型HPV—16所占比例显著高于Ⅰ期宫颈癌结论:1)多重实时定量PCR是一种敏感性高、简便快速的HPV—16病毒存在状态的检测方法2)HPV-16整合是宫颈癌变的早期事件,与宫颈癌前病变的进展有关。3)宫颈癌中整合型HPV—16的发生可能具有预后价值

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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