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On the basis of twin-roll thin strip continuous casting experiments and solidification structure characteristic analysis of thin strip and combining with the simulation results of effects of technological parameters, the forming mechanism of solidification structure zone (especially equiaxed crystal zone) on 1Crl8Ni9Ti stainless steel twin-roll thin strip was investigated.

在双辊薄带连铸实验和薄带凝固组织特征分析的基础上,结合对薄带凝固组织区的模拟预测结果,研究了1Cr18Ni9Ti不锈钢双辊薄带凝固组织区的形成机理。结果表明:1Cr18Ni9Ti不锈钢双辊薄带凝固组织中的等轴晶区不但在凝固类型为半固态时形成,在轧制或理想型时也能形成。其形成机理为,熔池中悬浮游离晶体的沉积、聚集以及在枝晶生长前沿的长大和薄带离开二铸辊最小间隙后,薄带/空气界面换热系数骤然降低抑制了柱状枝品的生长,并促使薄带中部未凝固熔体中游离晶体的择优长大

Methods: 1. Experimental laparoscopic liver resection: We selected pigs as experimental models aiming to approach human beings physiological and anatomical condition. After CO2 pneumoperitoneum was established, the ligaments around liver were dissociated. Livers were cut off by the methods such as clamp dissection, microwave tissue coagulator, ultrasound dissector, Ligsure dissection, hand-assisted, short abdominal incision laparoscopy-assisted, and Endo-GIA switcher. The operation time and bleeding volume were compared between different methods of laparoscopic liver resection. The advantages and limitations of different methods were summarized. 2. Clinical laparoscopic liver resection: 15 cases with tumors in segment V of anterior right lobe or segments II, III of left lateral lobe or segment IV of left internal lobe were selected in this study. The liver diseases included hepatocellular cancer, hepatic cavernous hemangioma and hepatic focal nodular hyperplasia. The ligaments around liver were dissociated after CO2 pneumoperitoneum established. Laparoscopic liver resection were carried out by some of the methods combined such as clamp dissection, microwave tissue coagulator, hand-assisted, short abdominal incision laparoscopy-assisted, ultrasound dissector, rotation and suction dissector.

1、实验性腹腔镜肝切除:以猪为实验动物,旨在接近人体的生理、解剖环境下进行实验,建立操作空间后游离肝脏周围韧带,采用钳夹分离切肝法、微波刀切肝法、超声刀切肝法、Ligsure切肝法、手辅助腹腔镜肝切除法、小切口腹腔镜辅助切肝法、Endo-GIA切肝法等方法实施腹腔镜下肝切除,比较不同切肝方法的手术时间、出血量,总结各种方法的优越性及其局限性。2、临床腹腔镜肝切除:选择肿瘤位于肝右前叶第Ⅴ段、肝左外叶第Ⅱ、Ⅲ段及左内叶第Ⅳ段的15例患者,病种包括原发性肝癌、肝海绵状血管瘤、肝脏局灶性增生,建立气腹后游离肝脏周围韧带,采用钳夹分离断肝、微波刀固化后断肝、手辅助腹腔镜肝切除、小切口腹腔镜辅助肝切除、超声刀断肝、旋吸断肝等多种方法分离切线肝组织,暴露管道结构,再采用高频电凝、钛夹夹闭、缝扎止血、止血纱布覆盖、氩气刀止血、生物胶止血、Endo-GIA处理等多种方法相结合处理肝断面,统计手术完成情况、手术时间、手术出血量、术后并发症及术后住院时间等,评价腹腔镜肝切除的可行性及安全性。

The absorption of root system was the main sources of nitrogen in the plant.The effects of matrix ventilation on the abaorption,transportion and metabolism of nitrogen in the root system of cucmber were discussed deeply.The results showed that contents of ammonium,nitrate,free amino acids and soluble protein in cucumber roots were higher in G1 treatment.Contents of ammonium,nitrate and free amino acids in cucumber roots were the trend of the dropping after the first rising in F1 treatment,but contents of soluble protein was lower11.03%than that of the control.

根系吸收是植物体内氮素的主要来源,本研究深入探讨了基质通气状况对黄瓜根系氮素吸收、运输和氮代谢的影响,结果表明G1通气处理根系的铵盐、硝酸盐、游离氨基酸和可溶性蛋白含量均较高;非通气处理(F1处理)根系内铵盐、硝酸盐和游离氨基酸含量呈先升高后降低的趋势,而可溶性蛋白含量始终较低,比对照降低11.03%。G1处理根系硝酸还原酶、亚硝酸还原酶、谷草转氨酶、谷丙转氨酶、谷氨酰胺合成酶活性均较高;而F1处理的酶活性在处理末期显著降低,其中谷氨酰胺合成酶活性比对照降低33.33%。

They were reversible un-competitive inhibitors, and their inhibition constants of free enzymeK(subscript I were 254.00 and 136.78 mmolL^(-1), respectively. Urea was reversible mixed-type inhibitor, and its inhibition constants of free enzymeK(subscript I and enzyme-substrate complexK(subscript IS were determined to be 379.02 and 1182.00 mmolL^(-1), respectively.

Na2SO3和H2O2对酶的抑制均表现为可逆反竞争性类型,它们对游离酶的抑制常数K(下标 I分别为254.00和136.78 mmolL^(-1);尿素对酶的抑制表现为可逆的混合型抑制,它对游离酶的K和对酶-底物复合物的抑制常数K(下标 IS分别为379.02和1182.00 mmolL^(-1)。

Results showed that the con- tent of phospholipids decreased from 23.3% to 11.2%,the content of free fatty acid increased from 1.6% to 11.5%,the content of glyceride had no significant change;SFA、MUFA and PUFA of glyceride and phos- pholipids had no significant change;in the free fatty acid,the content of PUFA significantly decreased,the content of MUFA were also decreased,but had no significant change,the content of SFA increased signifi- cantly.

结果表明:磷脂的含量从23.3%减少到11.2%,游离脂肪酸的含量从1.6%增加到11.5%,甘油酯的含量没有发生显著性变化;甘油酯和磷脂中的饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸都无显著性变化;游离脂肪酸中,PUFA的含量显著减少,MUFA的含量减少,但其变化不显著,SFA的含量显著增加。

But after ultra-high pressure treatment, the all free amino acid can be measured except alanine and cystine and the content of asparagines is lower. The content of free amino acid is the highest in ultra-high pressure sterilize milk, the pasteurism milk is second, the ultra high temperature sterilized milk is lowest.

但是牛乳经过超高压杀菌处理以后,除丙氨酸和胱氨酸未检出,且超高压杀菌处理以后的天门冬氨酸的含量略低以外,其余游离氨基酸均可检出,而超高压杀菌牛乳中游离氨基酸的含量最高,巴氏杀菌乳中的含量次之,超高温瞬时杀菌乳中的含量最低。

Operative design: After gastric anterior wall was exposed, any Crow's nerve was identified from antral surface nearby gastric angle. Along the Crow's nerve, Latarjet nerve was found, gastric branches of Latarjet were gradually severed, the dissection proceeds to up towards the diaphrageal fissula, and then, the right gastroepiploic neurovascular bundle was cut, the distal two-thirds of the greater curvature was mobilized by dividing the greater omentum, the posterior nerve of Latarjet may be seen and isolated in the same way.

手术设计:暴露胃前壁,自胃角附近的胃窦壁表面任意找到一支&鸦爪&支迷走神经,随以此支为标志向上找到分出该鸦爪的Latarjet氏神经干,顺该神经向上游离,分离中避开向右走行的肝支及胃小弯的主要血管,逐一切断走向胃壁的迷走神经分支,直游离到膈肌食管裂孔平面。

After extraction by ethanol and purification by cationic ion-exchange column, a method of using reversed-phase high-performance liquid chromatography and precolumn derivatization with o-phthalaldehyde and 9-fiuorenylmethylchloroformate is employed for determination of free amino acids in tobacco . This method assures that amino acids with amino and imino groups can be determined simultaneously. The results of duplication experiment and recovery experiment are acceptable.

本试验用乙醇提取烟叶中的游离氨基酸,并用阳离子交换树脂纯化,再用邻苯二甲醛、氯甲酸芴甲酯联合柱前衍生、反相高效液相色谱分析测定了烟叶中的游离氨基酸,该方法能同时测定烟叶中的带氨基和亚氨基基团的氨基酸,并且分析方法的重现性和回收率均令人满意。

In this paper, a total of 56 relatively nonpoint source pollution sediment samples were collected from the tidal flats of the Jiuduansha shoal and the Chongming island of the Yangtze river estuary. The main properties i. e., grain size distribution, contents of DCB (dithionite citrate bicarbonate extracted iron Fe(subscript d, AAO extracted iron Fe(subscript o, total Fe, DCB extracted Mn Mn(subscript d, total Mn, organic matter, magnetic susceptibility of the samples, as well as the concentrations of Cu, Zn, Pb, Cr, Cd and Ni in the samples, were determined. Basing on the data, the relation of the sediment properties to heavy metal concentrations was evaluated with pearson's rank and partial rank correlation analysis.

采集长江口无明显点源污染的崇明东滩和九段沙潮滩56个沉积物样品,测定沉积物原样和粘粒中Cu,Zn,Pb,Cr,Cd和Ni的含量以及沉积物的主要性状(粒径分布、游离铁、活性铁、总铁、游离锰、总锰、有机碳和磁化率等),以相关分析和偏相关分析研究沉积物中重金属含量与沉积物主要性质之间的关系。

It was demonstrated that the dependence or independence of recA gene is not related to the direction of replication of the plasmids or phages in the free state.

实验结果说明,依赖与否和它们在游离状态中复制方向无关,从而否定了recA基因的作用是把质粒或噬菌体的游离状态的单向复制发动机构改变为整合状态的双向复制发动机构这一假设。

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