游离基因

Methods DNA clone of AD8 and NL43 was respectively transfected to skin by Gene Gun, The expression of virus in the epidermal cells was observed by flow-cytometry and fluoroscope.

以基因枪转染AD8及NL4 3分子克隆至皮肤，流式细胞仪和荧光显微镜观察病毒在表皮细胞中的表达，磁分离术分离CD80 +和CD80 -的游离细胞，进而以PCR和逆转录酶检测游走细胞中HIV对CD+ 4 T淋巴细胞的感染和表达。

This gene is known as env, and in free-living viruses the protein derived from it is responsible for locking on to a cell's surface to allow the virus to enter the cell.

这一基因命名为"env"，在游离的病毒体内，由其编码产生的蛋白质负责铆钉到细胞表面以使病毒得以进入细胞内。

Objective To determine the effect of free fatty acid on the differentiation of 3T3-Ll cells and the expression of insig2 mRNA, to evaluate the factors which effect on the expression of insig2 mRNA and the differentiation of 3T3-Ll cells, and to explore the effect of insig2 in the differentiation and formation of adipocytes and lipogenesis.

目的 观察游离脂肪酸对3T3-L1细胞分化及insig2 mRNA基因表达的影响，进一步了解影响3T3-L1细胞分化成熟及insig2表达的因素，探讨insig2基因在脂肪细胞分化与代谢中的作用。

Haploid culture Different genotype varieties were used to study the anther and microspore culture in vitro.

单倍体培养本文以不同基因型材料进行胡萝卜花药培养和游离小孢子培养研究。

On the base of review the research development in tissue culture and genetic transformation, Comparative analyses were made on the callus induction and embryogenetic abilities of anther and non-pollinated ovules of rubber tree. Fluorescence detection was implemented with the different in vitro material of Hevea brasiliensis.

本研究在综述了橡胶树组织培养及遗传转化研究进展的基础上，系统地比较了橡胶花药和未授粉胚珠愈伤组织诱导及体细胞胚胎发生能力，对橡胶树不同离体组织进行荧光背景检测，将在植物胚胎发育时期起重要作用的BBM基因导入橡胶树，对电激法转化游离小孢子细胞以及农杆菌介导花药愈伤组织进行初步探索。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

The result was also confirmed by measuring the transcriptional levels of four hordein homologous genes in thebarley cells. Free proline participates in the synthesis of B3-Hordein, which plays a key rolein regulating the balance of the cellular proline.

而大麦(PC1163)细胞醇溶蛋白4个同源基因的转录水平的检测结果也证实了这一点，游离脯氨酸参与合成B3-Hordein，在降低细胞内脯氨酸含量并维持脯氨酸动态平衡方面起了重要作用。

As the bar gene is promoted by the CaMV35 promoter, in which there is a 19bp palindromic sequence could act as recombination hot spot and lead to DNA rearrangement, we presumed that the transgene recombination events happened during the integration course have generated the complex Southern patterns of bar gene expression cassette.

线形基因表达框的游离DNA末端和CaMV35S启动子中重组热点介导的转基因重组可能是导致bar基因表达框复杂整合的重要原因。

Objective To study the efficacy of combined use of rhBMP2/BCBand free periosteal graft in repairing segmental bony defects.

目的 探讨复合基因重组人BMP2(rhBMP2)的异种骨与游离骨膜联合移植修复节段性骨缺损的效果。

It was demonstrated that the dependence or independence of recA gene is not related to the direction of replication of the plasmids or phages in the free state.

实验结果说明，依赖与否和它们在游离状态中复制方向无关，从而否定了recA基因的作用是把质粒或噬菌体的游离状态的单向复制发动机构改变为整合状态的双向复制发动机构这一假设。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。