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And a fluorescence molecule beacon probe is designed between upstream and downstream priers, 5' end of probe can mark fluorescence reporter group FAM, and the 3' end markes fluorescence quenching group DABCYL, and 5' end and 3' end of the probe have 6 bases which can be reflected and complemented to make fluorescence molecule beacon probe do not give out fluorescence signal in free state.

在上下游引物中间设计一条荧光分子信标探针,探针的5'端标记荧光报告基团FAM,3'端标记荧光淬灭基团DABCYL,探针的5'端和3'端有6个碱基能反折互补,使荧光分子信标探针在游离状态下不发出荧光信号。

DMSO, an effective free radical scavenger, was used to treat the planarians before or after the paraquat treatment.

游离氧自由基清除剂DMSO被用来在百草枯处理前或后处理涡虫。

In mature stage,the organelle,such as dissociative ribosome,Golgi body,endoplasmic reticulum and nuclear,was disassembled quickly.

在此时期内,筛管内的游离核糖体、高尔基体、内质网、细胞核等细胞器迅速解体消失。

There are some little cells with diameter 4.5~8.3 μm in molecular layer. Purkinje cell is like a pear in Purkinje cell layer, it's base width is 17.8~25.6 μm, it's height is 18-30 μm. There are lots of rough endoplasmic reticulum, free ribosome, mitochondria, golgi body, lysosome, etc. in Purkinje cell. Granular cell ratio of nucleus to cytoplasm is high in granular layer.

分子层含少量小细胞,细胞大小在4.5~8.3μm; Purkinje细胞层细胞体呈梨状,底宽在17.8~25.6μm之间,高约为18~30μm,细胞中含有丰富的粗面内质网、游离核糖体、线粒体、高尔基体、溶酶体等;颗粒层的颗粒细胞细胞核大,细胞质少。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。