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A new strain of Brevibacterium casei with high nitrilase activity was screened from natural environment and could convert nitrile into carboxylic acid.

从自然源筛选到的一株腈水解酶产生菌乳酪短杆菌,能够高选择性地将腈转化为羧酸,初步研究了该菌株所产的腈水解酶在游离细胞形态下的催化特性,包括热稳定性、pH稳定性、金属离子和添加剂的作用、溶剂耐受性及底物特异性等。

The latter is chlorinated in the side chains under free-radical conditions to give 2,4-dichloro-5-fluoro-3-dichloromethyl-1-trichloromethylbenzene. The latter is hydrolysed via 2,4-dichloro-5-fluoro-3-dichloromethylbenzoic acid, which can be isolated if necessary, to give 2,4-dichloro-5-fluoro-3-formyl-benzoic acid, the aldehyde group of which is reacted to give 2,4-dichloro-5-fluoro-3-N-hydroxyiminomethyl-benzoic acid, from which, with simultaneous conversion of the carboxyl group into the chlorocarbonyl group, water is eliminated using an acid chloride to give the nitrile 2,4-dichloro-3-cyano-5-fluoro-benzoyl chloride.

随后,在游离基条件下进行侧链的氯化,得到2,4-二氯-5-氟-3-二氯甲基-1-三氯甲基苯、后者被水解,经过2,4-二氯-5-氟-3-二氯甲基苯甲酸(如果需要此化合物可以分离),得到2,4-二氯-5-氟-3-甲酰苯甲酸,将其醛基反应,得到2,4-二氯-5-氟-3-N-羟基亚氨基甲基苯甲酸,用酰氯从其中除去水,同时将羧基转化为碳酰氯,得到一种腈,即2,4-二氯-3-氰基-5-氯-苯甲酰氯。

The affinity constant of E1-McAb reacting with coated complete antigen was 8.2×10^8L/mol. Biotin-streptavidin complex was used for the determination of E1. The calibration curve of free E1 covered a range of 10~10 000 pg/mL.

以生物素化的羊抗鼠免疫球蛋白及辣根过氧化物酶标记的链亲和素为标记体系,通过竞争抑制的方式测定游离的雌酮,结果表明:雌酮在10~10000pg/mL内呈线性关系。

In the absence of coorperative effect, the reaction between lectin molecules and their receptors on cell surface can be described as below when equilibrium is reached:In which LT denotes total lectin concentration, LF free lectin concentration, mva-lency of lectin, ni number of receptors with affinity constant Ki to lectin, CT total lectin concentration.

本文提出一个描述细胞与多价凝集素反应的平衡关系式和用紫外分光光度法测定游离凝集素浓度求受体数量及其与凝集素的亲合常数的方便方法。

The Scatchard analysis of data revealed a linear relationship between the amount of specific binding and the ratio of specific binding to free 125 I (D Lys 6)LHRH, indicating a single class of high affinity sites. Equilibrium dissociation constant was 0 34 nM in pituitary membrane preparation and 0 43nM in isolated pituitary cells.

特异性结合随125 I( DLys6 ) LHRH 量的增加而增加,实验结果经Scatchard 分析揭示出特异性结合与结合和游离125I( DLys6)LHRH 之比呈线性关系,预示绍鸭垂体对( DLys6)LHRH 存在同一类高亲和力的结合位点。

The grain filling of superior spikelets was a continuous progressing process without interruption: Free nucleus→endosperm cell formation→endosperm cells differentiating into aleurone layer and starchy endosperm→composition and storage of reserve materials such as starch and storage proteins.

水稻强势籽粒的胚乳发育是一个无间断的连续过程,经过游离核期→胚乳细胞化→胚乳细胞分化发育成糊粉层和淀粉质胚乳→合成和累积淀粉、蛋白质等贮藏物质,约在20d以内完成胚乳发育的全过程,不同强势籽粒的胚乳发育进程大体一致。

The reaction conditions including the molar ratio of methanol to oil, amount of alkaline catalyst used, and reaction temperature and time were optimized by the mono-factor and orthogonal tests.

通过棉籽现场碱催化转酯化联产生物柴油和无毒棉粕,考察了棉仁中水的质量分数、粒径对产物中脂肪酸甲酯的质量分数和棉粕中游离棉酚质量分数的影响;对反应过程中的醇油摩尔比、反应时间、催化剂用量、反应温度进行了单因素和正交实验考察。

Under this condition the content of amino nitrogen was 59.2 mg/L after 3 hours of enzymatic hydrolysis.

枯草杆菌蛋白酶酶解鲤鱼后,游离氨基酸含量由185.91 mg/L增加到779.91 mg/L,增加了76.16%。

From the variance of free amino nitrogen,we can see that Lactobacillus protein degradation capacity will be greater than lactis.

从不同菌属乳酸菌在发酵过程中游离氨基氮产量的变化来看:乳杆菌蛋白分解能力明显高于乳球菌。

Results: The physiological and pathological characteristics of the old patients make the repair of the ulcers rather complicated. Change of dressings is the basis of all treatment, free skin grafting is the most widely applied and safe method among the surgical operations skin flap transposition is suitable for treating deep tissue exposure and those suspected of canceration. Amputation is indicated for those with extremities cancer and systemic amyloid degeneration.

结果:老年人特有的生理、病理特点决定了创面修复的复杂性,换药是一切治疗的基础,游离植皮是最安全、应用最广泛的手术方法;皮瓣转移适用于有深部组织暴露或疑有癌变患者;截肢适用于肢体远端高度疑有癌变或已引起全身淀粉样变者。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。