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And then HepG2.2.15 cellswere cultured with different non-cellulotoxic concentration ofα-amanitin or 3TC for six days.

采用MTT法检测不同浓度α-鹅膏毒肽对体外培养的HepG2 2.2.15细胞株的毒性,然后在无毒浓度下用不同浓度α-鹅膏毒肽或拉米夫定与HepG2 2.2.15细胞共培养6天,分别在第3天和第6天收集细胞培养上清液,采用时间分辨免疫荧光法测定上清HBsAg、HBeAg浓度;收获细胞提取总RNA,行逆转录聚合酶链反应检测细胞内HBsAg和HBcAg mRNA的表达。

Day, he one when hunting in the forest,he caughtsight of artemis'' fair maidens,the seven pleiades .hewas so attracted by their beauty that he started chasing them.indespair the charming maidens prayed to artemis for help and were sent up by the goddess to the heavens as a constellation .later he fell in love with another maiden,merope by name,butrash and impatient,he took the maiden by force and enraged her father,king oenopion of chios.the king made him drunk and put out his eyes .following the sound of the cyclopes'' hammer,he went to hephaestus,who sent a guide to travel with him to the east,where the sun rose.exposing his eyeballs to the rays of the sun,he was restored to his eyesight.he became aconstant companion of artemis and followed the goddess in the chase with his dog sirius.apollo did not approve of this love, however.one day he dared his sister to shoot at a dark spot moving in the sea.little did she suspect that she was aiming herarrow at orion,for the dark spot turned out to be the head of orion swimming in the ocean.artemis was extremely sad.as she could not pull him back from the grasp of death she sent him up to the sky as a constellation,with his hunting dog sirius be sidehim.

他是海神波塞冬漂亮的儿子。一天,他在森林中打猎,看到了阿尔特弥斯的仙女们——七颗明星。他如此爱慕她们的美丽,便追赶她们。困境中,美貌的仙女们向阿尔特弥斯求救。结果女神将她们都送入天空充当星座。后来奥利安爱上了另一少女墨洛珀。他鲁莽急躁地施暴于她。这激怒了少女的父亲俄诺皮耳国王。国王把他灌醉后,挖出了他的眼睛。随着"独眼巨人"大锤的敲响,他来到火神赫斐斯托斯跟前。火神派向导带他去太阳升起的东方。将眼球暴露于阳光后,他就恢复了视力,成了女神阿尔特弥斯永久的伴侣,并带着他的猎狗天狼跟随女神打猎。阿波罗并不赞成他们之间这种恋情。一天,阿波罗怂恿阿尔特弥斯向海中一移动的黑点射箭。她根本没想到自己是将箭瞄准奥利安。当看清黑点竟是在游泳的恋人奥利安的头时,阿尔特弥斯悲痛万分。因她无力使他起死回生,只好将他送入天空作一星座。他的猎狗天狼则蹲在他的身旁。

Orion orion was a giant hunter.he was the beautiful son of poseidon.one day ,when hunting in the forest,he caughtsight of artemis'' fair maidens, the seven pleiades .hewas so attracted by their beauty that he started chasing them.indespair the charming maidens prayed to artemis for help and were sent up by the goddess to the heavens as a constellation .later he fell in love with another maiden ,merope by name,butrash and impatient, he took the maiden by force and enraged her father,king oenopion of chios.the king made him drunk and put out his eyes .following the sound of the cyclopes'' hammer,he went to hephaestus ,who sent a guide to travel with him to the east,where the sun rose.exposing his eyeballs to the rays of the sun ,he was restored to his eyesight.he became aconstant companion of artemis and followed the goddess in the chase with his dog sirius.apollo did not approve of this love,however.one day he dared his sister to shoot at a dark spot moving in the sea.little did she suspect that she was aiming herarrow at orion ,for the dark spot turned out to be the head of orion swimming in the ocean.artemis was extremely sad.as she could not pull him back from the grasp of death she sent him up to the sky as a constellation, with his hunting dog sirius be sidehim.

奥利安奥利安是位身材高大的猎手。他是海神波塞冬漂亮的儿子。一天,他在森林中打猎,看到了阿尔特弥斯的仙女们——七颗明星。他如此爱慕她们的美丽,便追赶她们。困境中,美貌的仙女们向阿尔特弥斯求救。结果女神将她们都送入天空充当星座。后来奥利安爱上了另一少女墨洛珀。他鲁莽急躁地施暴于她。这激怒了少女的父亲俄诺皮耳国王。国王把他灌醉后,挖出了他的眼睛。随着"独眼巨人"大锤的敲响,他来到火神赫斐斯托斯跟前。火神派向导带他去太阳升起的东方。将眼球暴露于阳光后,他就恢复了视力,成了女神阿尔特弥斯永久的伴侣,并带着他的猎狗天狼跟随女神打猎。阿波罗并不赞成他们之间这种恋情。一天,阿波罗怂恿阿尔特弥斯向海中一移动的黑点射箭。她根本没想到自己是将箭瞄准奥利安。当看清黑点竟是在游泳的恋人奥利安的头时,阿尔特弥斯悲痛万分。因她无力使他起死回生,只好将他送入天空作一星座。他的猎狗天狼则蹲在他的身旁。

In addition, according to Qing Dynasty Gongbu archives, the author clarifies the construction of the Qintianjian yashu which possessed one hundred and ten rooms, including the 协政堂 xiezhengtang,主薄厅 zhuboting,五官厅 wuguanting,宪书房 xianshufang,司书厅 sishufang,时宪科 shixianke,天文科 tianwenke,漏刻科 loukeke for routine,板库; banku for storing the book boards and 斋戒房 zhaijiefang,土地庙 tudimiao, etc., for sacrifice and enshrinement.

清钦天监衙署廨舍情况则鲜见于史,笔者在查阅清档时,见到乾隆年间工部维修钦天监衙署的奏销黄册,知清钦天监衙署廨舍一百一十间,其中既有处理日常事务的协政堂、主薄厅、五官厅、宪书房、司书厅、时宪科、天文科、漏刻科;也有收贮书板的板库;还有祭祀供神的斋戒房、土地庙等。据此笔者弄清了清钦天监的衙署形制、廨宇规模。

In addition, according to Qing Dynasty Gongbu archives, the author clarifies the construction of the Qintianjian yashu which possessed one hundred and ten rooms, including the 协政堂 xiezhengtang ,主薄厅 zhuboting ,五官厅 wuguanting ,宪书房 xianshufang ,司书厅 sishuting ,时宪科 shixianke ,天文科 tianwenke ,漏刻科 loukeke for routine;板库 banku for storing the book boards and 斋戒房 zhaijiefang ,土地庙 tudimiao , etc., for sacrifice and enshrinement.

清钦天监衙署廨舍情况则鲜见于史,笔者在查阅清档时,见到乾隆年间工部维修钦天监衙署的奏销黄册,知清钦天监衙署廨舍一百一十间,其中既有处理日常事务的协政堂、主薄厅、五官厅、宪书房、司书厅、时宪科、天文科、漏刻科;也有收贮书板的板库;还有祭祀供神的斋戒房、土地庙等。据此笔者弄清了清钦天监的衙署形制、廨宇规模。

Furfur. At the same time, selected the culture supernatant. Used the whole-fat-cow"s-milk plate method, the Folin-phenol method and the QuantiCleave proteinase Assay Kit to test the proteinase activity of mycelial phase and yeast phase of M. furfur. The effect of temperature and pH on this assay was also evaluated.Result:①The whole-fat-cow"s-milk plate method: Seven days after inoculation, the proteinase activity of the mycelial phase supernatant (12.5 ± 1.2mm) is higher than that of the yeast phase (9.25±0.71mm)(p.05); With the passage of the inoculation time, the proteinase activity of the mycelial phase is rising; the temperature has no obvious effect on the proteinase

结果:①全脂牛奶平板法:培养第7天菌丝相上清液蛋白酶活性(12.5±1.2mm)高于酵母相(9.25±0.71mm)(p<0.05);菌丝相上清液蛋白酶活性随培养天数延长而增大;25℃和37℃两种温度对上清液蛋白酶活性无明显影响(P>0.05);②Folin-酚法:上清液蛋白酶活性随培养天数、菌落计数、菌丝诱导率增加而增大,培养液pH值呈下降趋势;同一天的菌丝相上清液蛋白酶活性明显高于酵母相(p<0.05);三种温度(25℃、37℃、49℃)及三种pH值(3.8、5.6、7.4)对上清液蛋白酶活性无明显影响;温度37℃、pH5.6时菌丝相上清液蛋白酶活性最高;③QuantiCleave蛋白酶试剂盒:菌丝相及酵母相上清液中均含有蛋白酶且

The half-life day of HCH, DDT and chlorpyrifos was 27~32, 27~33 and 22~24 respectively and the residue were detected in the substrates and fruitbodies when fungi were inoculated to the substrated, There were no cypermethrin detected in the fruitbodies, and it's half-life day in the substrate was 19~20. Carbendazim used for control germina was also detected in the substrates and fruitbodies with a half-life from 19 days to 20 days. Because of it's short half-life (2 days to 4 days), the degradation of Chlorothalonil in substrates and fruitbodie were quickly and no chlorothanonil were detected both in the substrates and fruitbodies when harves.

在接菌情况下,HCH、DDT和毒死蜱等杀虫剂在食用菌子实体和培养料中都有残留,培养料中的降解半衰期分别为27~32天、27~33天和22~24天;氯氰菊酯在培养料中的降解半衰期为19~20天,但在子实体中没有残留;多菌灵是防治食用菌病害的常用杀菌剂,在培养料和子实体中也都有残留,并且培养料中的降解半衰期为19~20天;百菌清极易降解,在培养料和子实体都没有残留,在培养料中的降解半衰期仅为2~4天。

Professional Deputy Imports: Products provide all customs clearance of imports (full-day customs clearance documents, rapid clearance), pay the foreign exchange (loans; freight), pay the tariff; value-added tax, VAT invoices can be issued in full, such as the import agent for the whole all import procedures!

专业代理进口:提供所有产品进口报关清关(单证齐全一天清关,快速清关)、代付外汇、代缴关税;增值税、可开具全额增值税发票,等全程进口代理提供所有进口手续!

Animals were sensitised with 10 μg or 100 μg OA, as either a single or booster (day 1 and day 5) injection.

本研究的目的是为了优化卵清蛋白的致敏条件,以实现一个豚鼠哮喘模型的早期哮喘反应,后期哮喘反应,气道高反应性和细胞浸润,使用10 μg或为100 μg的卵清蛋白敏化动物,或者采用单一的或强心针(1天和5天)注射。

In 1980, by Zhao Puchu, president of the proposal introduced by the Chinese Buddhist Association of Master Ching scheduled to Zhejiang Tiantai Guoqing Temple preaching preaching. In 1983, the Qing Chengdu, Zhao Juesi set by the Master of all the monks ceremony invitation, abbot Zhaojue Si.

1980年,经赵朴初会长提议,由中国佛教协会介绍清定法师到浙江天台山国清寺讲经弘法。1983年,清定法师受成都昭觉寺全体僧人礼请,住持昭觉寺。

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