添加生长

Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG. The solubility of inclusion bodies of rCPB in 10mol/L urea was 2~3 times higher than in 8 mol/L urea, and the renature solution with 0.75%β-ME in 10mol/L urea could increase the solubility of inclusion bodies of rCPB, comparing with that in 10mol/L urea simply.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响；rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍；添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

First, the fermentation of residue with Saccha romyces cerevisia was studied. In the process of fermentation, the effects of temperature, initial pH, inoculums size, carbon and nitrogen addition , cultivation time were discussed.

通过对残渣成分的分析，以及温度、初始pH、接菌量、添加C、N源及发酵时间等对单菌发酵残渣时啤酒酵母生长的影响，得出：在35℃、初始pH6.0、接菌量10%、发酵24h的条件下，粗蛋白含量由发酵前的5.3%提高到发酵后的6.5%，提高幅度为22.6%。

The multicopy gene saccharomycetes expressed recombinant plasmid pAOnsGH, the experiment shows, has very high expression activity to make the engineering saccharomycete product possess low production cost and high efficiency. Trial raising shows that feed with the said saccharomycetes added can raise the growth speed, disease resistance and output of the raised animals.

多拷贝基因酵母菌表达重组质粒pAOnsGH，经实验证明具有很高的表达活性，使本工程菌制品实现了生产成本低、效率高的效果，经多次水产养殖试验及养鸡试验，使用添加本酵母菌制品的饲料饲，喂养殖的动物可提高其生长速度和抗病害能力，提高单位养殖产量20％以上，增产的收入大于本制品成本投入的五倍以上。

Three-dimensional Matrigel constructs contained aleolar forming units comprised of cells displaying AE2 cellular ultrastructure while expressing the SpC gene and gene product. The addition of tissue-specific growth factors induced formation of branching, sacculated epithelial structures reminiscent of the distal lung architecture.

通过对细胞亚微结构的观察和对SpC基因及其表达产物的分析，研究者发现培养体系中肺组织形成单位中具有能向肺泡II型上皮细胞分化的细胞；而通过添加组织特异性的生长因子，他们还发现这一做法会促进培养组织形成具有分支和囊状上皮结构的类似终末肺泡组织的形态结构。

Addition of wastewater or nutrientsto lake water in various doses had stimulated the growth of alga Scenedesmus obliguus.

在东湖湖水样品中添加排入东湖的主要污水或营养物进行藻类测试，观察它们对斜生橱藻的生长促进作用。

Adding purified stylar S-RNase from Prunus mume cultivar 'Gessekai' to liquid medium, for cultivating self- and cross-pollen at temperatures lot, 25℃ and 35℃, respectively, then determining the rate of pollen germination and the length of pollen tube after different periods of cultivating time.

在液体培养基中添加从果梅'月世界'花柱中纯化的S-RNase，并分别于10℃、25℃和35℃条件下培养自花、异花花粉，测定不同培养时间后的花粉萌发率和花粉管生长长度；离体条件下进行果梅花柱S-RNase降解花粉管RNA反应后，用琼脂糖凝胶电泳检测不同温度条件下自花、异花花粉管RNA被降解的程度。

Studies on midum kind, hormones combination, additives juice, sugar concentration of Thesium Chinense Turcz. The result shows that the best medium of inducting lateral shoot is MS+6-BA2.0mg/L+NAA0.5mg/L and the juice of Cynodon Dactylon Pers.

通过对白蕊草组织培养的培养基种类、激素配比、添加物汁液、碳源的研究，得出适合白蕊草侧芽分化生长的最佳培养基为MS+6-BA2.0mg/L+NAA0.5mg/L+狗牙根汁液。

Nine medium compositions were used to study their effect on I40 using Plackett-Burman design. The production of biomass for I40 was affected in order of yeast extract, tryptone, glucose, and production of protease affected in order of feather, tryptone and yeast extract.

最是化条件探讨方面，於基础培养基中分别添加九种单因子，以PB design分析，结果发现对於I40生长影响较为显著的因子依序为：yeast extract、tryptone、glucose，而对於该菌生产蛋白酶产量影响较为显著的因子依序为：feather、tryptone、yeast extract。

Versicolor suited to grow and produce laccases at pH value 3.5-5.7, and unbuffered media were able to get good laccase productivity though buffered media could get higher laccase activity. Metal ions Cu(superscript 2+) and Mn(superscript 2+) in the culture could remarkably increase the activity of laccases. The optimal concentration of Cu(superscript 2+) was 0.008-0.08mmol/L, and the optimal concentration of Mn(superscript 2+) was about 0.01mmol/L, when the culture contained 0.1mmol/L 2,5-dimethoxyaniline.

结果发现该菌种适宜在pH值3.5~5.7环境下生长并合成漆酶，培养基中添加缓冲液可以获得更高的酶活，但不加则有较高的酶产率；铜和锰可以显著提高漆酶活力，当以0.1mmol/L 2，5-二甲基苯胺为诱导剂时，培养基中宜含0.008~0.08mmol/L Cu(上标 2+)，0.01mmol/L左右的Mn(上标 2+)。

And probable mechanism for the solubility differences was illuminated by the analysis of unreduced SDS-PAGE. Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响；rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍；添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。