添加生长

In vitro, we cultured human and rabbit retinal Müller cells by way of mixed enzyme digestion and tissue adherence, using human retina of an abortus of 6 months and healthy rabbit.

取孕6个月引产胎儿，死后2h内无菌条件下摘取眼球，剥取视网膜组织，混合酶消化法培养人视网膜Müller细胞；取健康新西兰大白兔，死后以最快的速度无菌条件下摘取眼球，组织块贴壁法培养兔视网膜Müller细胞；利用含胎牛血清的高糖DMEM作为培养液，不添加生长因子。

NO3-N is the most popular and safest nitrogen source to Spirulina, the suitable concentration is about 11mmol/L, which can speed the growth rate and reduce the cost of cultivation as well. NH4-N of the suitable concentration can enhance the growth of Spirulina,but overhigh concentration will cause the ammoniac toxicosis, the suitable concentration of NH4-N is about 1.27～2.57mmol/L.

NO3-N对螺旋藻是最为通用和安全的氮源，但添加浓度以11mmol/L左右最为适宜，既可满足藻体的最佳生长需求又可降低养殖成本；适宜浓度的NH4-N可促进螺旋藻的生长，浓度过高则会造成NH3中毒，NH4-N的添加量以1.27～2.57mmol/L范围最为适宜。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

The results showed that the growth of root tips in liquid medium was better than that in agar-solidified medium and solid-liquid two phase medium, for that increasing agar concentrations in medium will reduce the root growth; darkness was beneficial for Fraxinus mandshurica root tip culture in vitro; as the carbon sources, sucrose was better than glucose and maltose as the carbon ...

结果表明，不添加琼脂的液体培养基对根尖的生长较添加琼脂的固体培养基和固—液培养基好，原因是增加琼脂的量会抑制根的生长；暗培养比光培养更有利于水曲柳离体根尖的培养；蔗糖作为碳源的效果较果糖和麦芽糖好，其中以3%的蔗糖对离体根尖的生长效果最好。

Methods of removing of zone pellucida and addition of growth factors to the culture medium had no effect on the efficiency of production of monozygotic twins.

这些结果表明，从F1品系可更有效地产生同卵孪生子；不同的去透明带方式和添加生长因子与否对产生同卵孪生小鼠的得率没有明显差异。

After transfer of 13 twin blastocysts to recipients, 3 and 2 twin fetuses were obtained, These findings indicate that monozygotic twin mice can be produced more efficiently from F1 embryos than from CD-1 embryos.

在添加生长因子与未添加生长因子的胚胎中，分别有92％和86％的胚胎发育为孪生囊胚。13对胚胎移植后，分别产生了3对和2对孪生小鼠。

In 75m in the South China Sea, Fe enriched from a large range of concentrations stimulated the growth of nanoplankton. For the picoplankton, Fe enrichment of nano-molar level increased the cell abundance of Euk, on the other hnad, Syn and Pro had significant responses to higher concentration of Fe enrichment.

75米层海水铁添加实验表明，不同浓度的铁均促进nano-级的浮游植物的生长；对于超微型浮游植物来说，纳摩尔级铁的添加有利于超微型真核生物的生长，而更高浓度的铁添加则对聚球藻和原绿球藻的生长有利。

The results show that though the strain is growing slower in the nitrogen-free medium than in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium. The capsular polysaccharides produced by the strain in the culture which contains different mineral powders will reach the highest content on the third or the fourth day in its growing period. The strain's capability of releasing potassium from k-feldspar and biotite in the nitrogen-containing medium is higher than that in the nitrogen-free medium because in the nitrogen-free medium, the strain and its production of glucoprotein are less than those in the nitrogen-containing medium.

结果表明，尽管该菌在无氮培养条件下的菌体数量远小于有氮培养条件，但无氮培养条件下所提取的细菌多糖多于在有氮条件下培养所提取的细菌多糖；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的无氮培养基中生长可形成大量多糖，采用丙酮法进行细菌培养液多糖提取，发现细菌培养的第3天所提多糖含量最高；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的有氮培养基中生长亦可形成较多的多糖，且在细菌培养的第4天所提多糖含量最高；胶质芽孢杆菌在有氮条件下对含钾矿物的释钾率高于在无氮条件下的释钾率，这可能与该菌在有氮条件下生长更快、可产生较多菌体细胞有关。

It is conducive to uptake soil nitrogen for flue-tobacco on dryland soil with maize-tobacco rotation before topping (60 days after transplanting) and uptake soil nitrogen for flue-tobacco on paddy soil with rice-tobacco rotation after topping.2 The nitrogen utilization efficiency of rapeseed cake, rice straw and rape straw were 19.5%, 15.5%, 8.1% respectively, and the inorganic fertilizer nitrogen utilization efficiency were 41.1%, 42.7% and 35.7% under the combined application of organic additives and inorganic nitrogen fertilizer. The contribution rate of rapeseed cake, rice straw, rape straw to accumulated nitrogen of flue-cured tobacco were 1.0%, 2.4%, 2.7% respectively by the combined application of organic additives and nitrogen fertilizer. Flue-cured tobacco for organic nitrogen absorption meet with the nitrogen needs laws of high-quality tobacco, indicating that the combined application organic additives and inorganic nitrogen fertilizer reduces the nicotine content of tobacco, an increase of sugar/nicotine ratio and improved the quality of tobacco. In which the role of rice and rape straw is greater than the rapeseed cake can be used as measures to lower nicotine.3 Nitrogen accumulation in flue-cured tobacco with the total nitrogen input was a significant positive correlation during the growth stage of flue-cured tobacco. The total nitrogen input for the 156.3-405.5kg.hm-2, in which the soil nitrogen mineralization capacity account for 22.6%-54.3%, with an average of 34.5%, that is, soil nitrogen mineralization accounted for 1/3 of the total nitrogen input.

旱地轮作土壤有利于烤烟生长前期对土壤氮素的吸收，水旱轮作土壤增加了烤烟生长后期对土壤氮的吸收。2在有机添加物与无机氮肥配施条件下，烤烟对有机添加物中氮的吸收规律基本符合优质烟的需氮规律，其中烤烟对菜籽饼肥、稻草秸秆、油菜秸秆所含氮素的利用率分别为19.5%、15.5%、8.1%，所配施无机氮肥的利用率分别为41.1%、42.7%和35.7%，菜籽饼肥、稻草秸秆、油菜秸秆对烤烟氮素累积量的贡献分别为1.0%、2.4%、2.7%。3不同轮作方式及添加有机物条件下氮素平衡结果显示，烤烟生长期间的氮输入总量为156.3-405.5kg.hm-2，其中矿化氮量为输入总氮量的22.6%-54.3%，平均为34.5%，约为输入总氮量的1/3；肥料氮的利用率为31.4%-42.7%；植烟土壤氮素表观损失率在37.5%-57.2%，平均为46.9%，约为输入总氮量的一半。

Some strains of the nematode-bearing bacteria were bioassayed for their parasitism. All these bacteria can totally not grow or grows badly on the media incorporating the extracts of pine trees, indicating some substances existing in the trees will harmful to the bacterial strains, which is further convinced by preliminary inoculation showing that single bacterial species will not infect Pinus thugbergii. Hence, the growth and reproduction of the nematode-carrying bacteria, which could produce toxic substances causing the death of the tree cells, is closely related to the nematodes, when they first enter to the trees. In the same time, the activity of bacteria improved the circumstances for which nematodes are living, which in turn will beneficial to the growth of nematodes. Thus, there is a complicated interaction among nematodes, bacteria, and pine trees.

对松材线虫携带的细菌的部分菌株进行了寄生性测定，表明这些细菌在添加松树提取液的细菌培养基中不能生长或基本不能生长，这说明松树体内可能存在某些不利于细菌生长的物质，同时初步的接种试验证实，单独的细菌接种不能感染健康的黑松，因此，线虫携带的致病细菌在最初进入树体时，其生长和繁殖与线虫密切相关，结合细菌产生的毒性物质可造成黑松细胞死亡的情况，说明细菌的生长与线虫有关，同时，细菌的活动改变了线虫在松树内的生长环境，使环境变得有利于线虫的生活，因此，线虫、细菌和松树三者存在着复杂的生物关系。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。