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The optimum brewing technology of the apple and pear complex wines was as follows: the ratio of the apple juice and pear juice was 2:1, the addition of SO2 40 rag/L, the sugar degree 20°Bx, the inoculation amount of yeasty 0.9 g/L, the initial fermentation temperature 20℃ and the fermentation time 10 d, the pest fermentation temperature 15-18℃ and the fermentation time 30 d.

苹果、梨复合酒的最佳酿造工艺为:苹果汁、梨汁的配比为2:1,SO2的添加量为40mg/L,可溶性固形物调至20°Bx,酵母菌的接种量为0.9g/L,前发酵温度为20℃,发酵10d,后发酵温度为15~18℃,发酵30d。

While for the solution without urea, no combustion reaction took place and a badly agglomerated precursor was formed.

而没有添加尿素的溶液中没有燃烧反应发生,合成的前驱物为比表面积低的块状团聚体。

When alkalescent extract of tea waste was added into UFresin,the bonding strength of plywood was raised.

脲醛树脂中添加茶叶废料弱碱抽提物时,胶合板的胶合强度升高。

This article studied the characteristics of substance and energy in rice-duck-azolla integrated farming system. RDA system increases food-chain complexity and system stability because ducks and azolla are incorporated into rice paddy systems.

对稻鸭萍共作生态系统物能流特征的分析结果表明,通过在稻田生态位添加鸭子和绿萍,使原来的稻田食物链结构趋向复杂化,增加了稻田生态系统的稳定性。

According to these reaction conditions, a low aqueous organic biphase system for the continuous production of Naproxen was developed.

反应在一个具有回路的连续流搅拌反应器中进行,反应器中添加有采用吸附法固定化的脂肪酶,载体为一种弱极性的合成载体,水相连同固定化酶颗粒一起永久保持在反应器中,有机流动相带入底物,带出产物。

Chelator-assisted hydrothermal method for growing ZnO nanopricks ZnO nanopricks were formed while 0.3 mol/L diaminoethane, 3 mol/L butylamine and 0.25 mol/L EDTA were used as chalators to react with zinc foil at 100℃ for 12 h.

络合物水解法制备ZnO球形颗粒利用酒石酸锌的水解反应,在水溶液中制备了由超细颗粒组装的ZnO球形颗粒,XRD、HRTEM检测结果表明颗粒为纤锌矿ZnO多晶结构,在溶液中添加乙二醇,产物的尺寸和分散性得到了改善。

Use some method of cered products、fermentation、isolation and screening ,that can product the black and red liquid of fermentation,and then make Black Rice Cake as well.

黑米经膨化、糖化、过滤,可得紫黑色糖化液,添加面粉等物,即能制出光亮细腻、口感香脆、富锌健脑的饼干——黑米饼干。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实:两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用,并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著,最佳效应浓度为400nM;2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响:①CREG蛋白对VSMC迁移的影响:刮伤实验发现,加入最佳效应浓度的wtCREG和mCREG蛋白24h后,OB2组迁移能力下降,HITASY组无明显变化;细胞外基质金属蛋白酶-2,9(Matrix metallo-proteinase 2,9,MMP2 ,9)明胶酶电泳检测和Western blot检测结果证实,两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2,9减少,而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases,TIMPs)增加;②CREG蛋白对VSMC分化的影响:加入400nM的wtCREG和mCREG蛋白12h后,OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加,LM-1、FN表达减少;③流式细胞仪分析细胞周期和BrDU染色分析证实,加入400nM的wtCREG和mCREG蛋白后,OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572,HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034;3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用:①免疫共沉淀和免疫荧光双染色分析结果显示,CREG蛋白与M6P/IGF2R存在直接结合;②应用抗体阻断实验:将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中,CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱,而且与加入anti- M6P/IGF2R浓度正相关。

Results The in vitro coupled bacterial luciferase:FMN-NADH oxidoreductase bioluminescent system was: 1mL crude extract, 27 mmol/L Dodecane 100 μL, 10 mmol/L FMN-Na 0.5 μL and 0.14 mmol/L NADH 300 μL.

利用从鳆发光杆菌提取并经部分纯化的荧光素酶和FMN-NADH氧化还原酶,通过优化体系中各底物的添加量,实现荧光素酶的体外发光。

The results showed that the higher peptides yield was 38.96mg/g on the conditions of 1% brown sugar power,initial pH7.5,temperature 30 ℃,rotational speed 180 r/min and the substrate 7%,which was fermented 48 h.The higher yield of free amino acids was 0.344% when ferme...

结果表明,发酵培养基碳源为红糖粉添加量1%,发酵初始pH 7.5,发酵温度30℃,转速180 r/min以及底物浓度7%,发酵48 h时多肽含量达到38.96 mg/g,当发酵时间持续到72 h可得到较多游离氨基酸,含量为0.344%发酵48 h发酵液的香味较好。

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在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

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