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Flow cytometry was used to investigated the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A,the mechanism of the effects of polysaccharide on the immune system was explored and theoretical and experimental evidence for the clinical application of Millettia Speciosa Champ in treating chronic diseases were drawn.

提取纯化多糖,流式细胞术检测多糖对刀豆蛋白A刺激引起的小鼠T淋巴细胞增殖的影响,探讨牛大力多糖对免疫系统的调节作用机制,为临床用牛大力治疗多种慢性疾病提供理论和实验依据。

Methods The polysaccharide was extracted and purified from Millettia Speciosa Champ,CFDA-SE staining and flow cytometry were used to detect the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A.

提取纯化多糖,流式细胞术检测多糖对刀豆蛋白A刺激引起的小鼠T淋巴细胞增殖的影响,探讨牛大力多糖对免疫系统的调节作用机制,为临床用牛大力治疗多种慢性疾病提供理论和实验依据。

Flow cytometry was used to investigated the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A,the mechanism of the effects of polysaccharide on the immune system was explored and theoretical and experimental evidence for the clinical application of Millettia Speciosa Champ in treating chronic diseases were drawn.

提取纯化多糖,流式细胞术检测多糖对刀豆蛋白A刺激引起的小鼠T淋巴细胞增殖的影响,探讨牛大力多糖对免疫系统的调节作用机制,为临床用牛大力治疗多种慢性疾病提供理论和实验依据作者:郑元升,蒲含林,麻建军目的从牛大力Millettia Speciosa Champ。

There are abnormal phenotypes of AChR-specific-responding T、B cell subpopulation in the myasthenic thymus, especially the activated Th cells. In addition,CD5- B cells might be related to the pathogenesis of the MG.

MG患者胸腺存在着异常的AChR特异应答性T、B淋巴细胞亚群表型,尤其以活化的Th细胞为著;CD5-B细胞的产生可能与MG密切相关。

Objective: To investigate the expression of Id1 gene in leukemic leukocyte,and determine its relationship with the pathogenesis of acute non-lymphocytic leukemia and chronic myelocytic leukemia,so as to provide some clues to their diagnosis, treatment and prognosis.

目的:研究分化抑制因子-1(inhibitors of differentiation,Id1)在白血病白细胞中的表达,探讨其与急性非淋巴细胞和慢性粒细胞白血病发病的相关性,为白血病的诊断、治疗提供参考。

Results The ACP reaction products were seen in the perinuclear region of the spreading blood cells, but they were not seen in the round blood cells.

结果 在钉螺血淋巴细胞中的伸展性阿米巴细胞胞浆中可见呈棕色颗粒的ACP阳性反应物,而圆形阿米巴细胞胞浆中未见呈棕色颗粒的ACP阳性反应物。

Rechallenge was performed 60 days after first tumor inoculation, all mice showed complete protection, indicating that the DC-gp100 induced specific anti-melanoma memory CD8+CTL.

为了验证NV抗原表位可以降低gp100特异的CD8+T 细胞活化阈值,我们先将NV抗原肽负载DC,接着再分别负载不同浓度梯度的gp100抗原肽,以此免疫小鼠,7天后以淋巴细胞增殖实验判断gp100特异的CD8+T 细胞活化阈值。

To observe the change of behavior index and mensurate the contents of plasma CORT, CRH and ACTH by using radiation immunity methods, and observed the change of immunity function by testing IL-2 and lymphocyte multiplication produced by spleen cell. At the same time, the author examined the change of thalamencephalon CRH mRNA and pituitary ACTH mRNA.

观察行为学指标的变化,利用放免法测定血浆皮质酮、下丘脑促肾上腺皮质激素释放激素和血浆促肾上腺皮质激素的含量,并通过脾细胞产生白细胞介素2(IL-2)水平和淋巴细胞增殖试验观察大鼠免疫功能的变化,同时检测大鼠下丘脑CRH mRNA和垂体ACTHmRNA含量变化。

It proliferates into clones that produce a continuous supply of a specific antibody .

杂种在实验室里制造的一种由一个能产生抗体的淋巴细胞与一个骨髓瘤结合而成的。

The membrane lipid fluidity of infected cells appeared obviously low-There were significant differences statistically(p.05-0.01)(2)By cytohisto-chemical stain-ing and microspectrophotometer quantitating,before cultured 72 hours,ACP and SDH in infectedcells raised.

动态观察1、6、24、72h培养后的淋巴细胞、单核细胞、中性粒细胞,发现感染后细胞膜脂的流动性明显降低,并随培养时间的延长而明显,和正常对照组比较有显著性差异(P<0.05~0.01)。

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