淋巴细胞的

Methods: hMSCs were separated from human marrow with Ficoll Paque reagent or direct culture and expanded in different culture medium with newborn bovine serum. The proliferation and growth characteristics were observed in primary and passage culture.

采用Ficoll Paque淋巴细胞分离液和全血接种两种方法分离成人MSC，筛选体外培养hMSC适合的培养基和适宜的血清含量，流式细胞仪检测hMSC表面抗原表达。

Methods Colony-forming unit of spleen, number of nucleated cells in bone marrow and spleen index were used to investigate the effect of polysaccharides at 50, 80 mg/kg from parmelia tinctorium intraperitoneal injection on hematopoietic function of mice irradiated with 137Cs γ-rays; DNA double-strand breaks were detected using alkaline single cell gel electrophoresis.

用内源性脾结节形成，骨髓有核细胞计数，脾脏指数观察石花多糖50，80 mg/kg腹腔注射对137 Csγ射线照射小鼠造血功能的影响；单细胞凝胶电泳技术检测小鼠淋巴细胞照射后的DNA双链断裂，观察给药后小鼠DNA损伤修复情况。

METHODS: the supernatants of cultured spleen's lymphocytes induced by ConA were injected into mice peritoneally,kinetics of plasma corticotropin releasing hormone,adrenocorticotropic hormone and corticosterone in mice were tested at different time (0.5,1,2,4,6 h),or tissue of hypothalamus pituitarium or adrenal was stimulated with supernatants induced by ConA,and the secretion of CRH, ACTH and CORT were observed.

采用ConA诱导淋巴细胞培养上清液给小鼠腹腔注射，测定不同时间(0.5，1，2，4，6 h)的小鼠血浆中CRH，ACTH，皮质酮产生的动力学，同时在体外用ConA诱导上清液分别与下丘脑，垂体，肾上腺组织共同培养后观察CRH，ACTH，CORT分泌情况。

Result The cultured DC from mouse bone marrow displayed the typical morphological characteristics of DC. Immature DC, which had high expression in CD11c and low expression in MHCⅡ and CD86, could phagocytize antigen. Mature DC, which had high expression in CD11c, MHCⅡ and CD86, could stimulate allogenic mixed lymphocyte proliferation.

未成熟DC的细胞表型为CD11c、MHCⅡ、CD86，具有极强的抗原吞噬能力；未成熟DC经LPS刺激培养后可转变为成熟DC，细胞表型为CD11c、MHCⅡ、CD86，可显著刺激同种异体混合淋巴细胞增殖。

Objective To explore the possibility of pharmacodynamic monitoring of cyclosporine immuno-suppression by measuring T-cell functions in peripheral whole blood.

目的：以不同的T淋巴细胞功能标志物作为药效动力学指标。探讨环孢素免疫抑制作用监测的可能性。

Results While the ES lumen and perisaccular region were infiltrated with mononeuclear-phagocyte at the 3rd day post-vaccination, plasmocyte and lymphatic cells become the predominant infiltrating cells in the ES at the 7th day. The KLH in the ES lumen were phagocytized at the 3-7th day post-vaccination. S-phase cells and IgG positive cells in ES increased markedly in the 3rd day and 7-14 days post-vaccination respectively with similar localization.

结果 内耳受抗原刺激后3 d内淋巴囊囊腔和囊周区域出现单核吞噬细胞浸润，7 d内淋巴囊的浆细胞和淋巴细胞大量增加；3～7 d内淋巴囊囊腔KLH被吞噬，同时S期细胞和IgG阳性细胞逐渐增多，二者的出现部位相似。

Results While the ES lumen and perisaccular region were infiltrated with mononeuclear-phagocyte at the 3rd day post-vaccination, plasmocyte and lymphatic cells become the predominant infiltrating cells in the ES at the 7th day. The KLH in the ES lumen were phagocytized at the 3-7th day post-vaccination.

结果 内耳受抗原刺激后3 d内淋巴囊囊腔和囊周区域出现单核吞噬细胞浸润，7 d内淋巴囊的浆细胞和淋巴细胞大量增加；3～7 d内淋巴囊囊腔KLH被吞噬，同时S期细胞和IgG阳性细胞逐渐增多，二者的出现部位相似。

Methods Routine immunization of BALB/ c mice by a tansfected cell line 293T/ B7-H4 as antigens.Then the immunized spleen was fused with SP2/0,a kind of plasmocytoma cells, and screen the positive clones by FCS with the 293T/B7-H4 as a positive control while 293T/ mock as the negative control.

以高表达人B7-H4分子的基因转染细胞株293T/B7-H4为免疫原，常规免疫BALB/c小鼠，采用B淋巴细胞杂交瘤技术进行细胞融合，并以该基因转染细胞293T/B7-H4作为阳性筛选细胞，以转空质粒的对照细胞293T/Mock作为阴性对照细胞。

The relationship of DNA ploidy level and SP-F with age,clinical stage, ascites, histologic type,pathological grade,lymphocyte infiltration and psammoma bodies was analyzed.

作者采用流式细胞技术，对24例新鲜速冻卵巢癌组织的细胞DNA含量及S期比例(S-phasefraction，SPF)进行了定量测定，分析了它们与患者年龄、临床分期、有无腹水、组织学分类、病理分级、肿瘤组织淋巴细胞浸润及砂粒体形成之间的关系。

Clinical features of measles in infants and children: time of fever was long, peak temperature of the fever was high, rash onset time long, more complications (especially to the younger infants and children); the main complications were pneumonia and enteritis; one patients died with death rate of 1.12%(1/89); compared the patients treated with antipyretics frequently and less frequently prior to hospitalization, the time of rashing and phase of fever had significant difference between the different treatment before hospitalization (P.05); 50 patients were diagnosed as pneumonia or bronchitis, 40% with reduction in absolute lymphocyte.

结果：本组流动人口占44.94%(40/89)；年龄组以3岁以下的嬰幼儿为主，占71.91%(64/89)，其中8月龄～3岁占56.18%(50/89)；符合麻疹计划免疫接种条件75例，接种率为26.67%(20/75)，流动人口接种率为7.5%(3/40)； 74.39%(61/82)病例发病前均有多家医院就诊史，临床特点：热程长，体温高峰较高，出疹期时间延长，并发症较多；并发症仍以肺炎、肠炎为主；死亡1例，死亡率为1.12%(1/89)；院外未频繁使用退热药组和频繁使用退热药组的出疹期和热程比较有显著性差异，P.05；本组50例诊断为肺炎或支气管炎， 40%淋巴细胞绝对值减少。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。