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Objective To study the changes in bone marrow megalokaryocyte numbers, platelet function and T-lymphocyte subsets in children with idiopathic thrombocytopenic purpura after treatment and normalization of platelet count.

目的 研究特发性血小板减少性紫癜患儿经治疗血小板数量正常后的血小板功能、巨核细胞数量及T淋巴细胞亚群的变化。

Of conclusion leukaemia patient outside configuration of week blood white blood cell and structural change can pass VCS parameter to determine mensurably, if partial parameter is neuter distributinging breadth of bead cell volume, lymphocyte is average bulk is leukaemia the sieve is checked, diagnosed new index.

结论白血病患者的外周血白细胞形态和结构变化可以通过VCS参数定量地测定,部分参数如中性粒细胞体积分布宽度、淋巴细胞平均体积是白血病筛查、诊断的新指标。

Methods The polysaccharide was extracted and purified from Millettia Speciosa Champ,CFDA-SE staining and flow cytometry were used to detect the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A.

提取、纯化牛大力多糖,利用CFDA-SE染色,流式细胞术检测多糖对Con A刺激引起的小鼠T淋巴细胞增殖的影响。

Biological activities were assayed with human peripheral blood mononuclear cells.

以人的外周血单核淋巴细胞测定两重组蛋白的生物活性。

ABSTRACT : Objective The phenotypes of the T、B lymphocyte subpopulation in myasthenic thymus were studied and the correlation of the peripheral blood and thymus were analyzed.

目的 研究重症肌无力(myasthenia gravis,MG)患者胸腺的T、B淋巴细胞亚群表型,并分析与其外周血的相关性。

To appraise effect of the home-made recombinant human granulocyte colony stimulating factor in the mobilization peripheral blood stem cells. From 2001 to 2005, 20 patients undergoing autologous peripheral blood stem cell transplantation were enrolled, 11 males, 9 females, 11 patients with acute myelocytic leukemia, 5 patients with acute lymphocyte leukemia, 3 patients with malignant lymphoma, and 1 patient with multiple myeloma.

为评估国产重组人粒细胞集落刺激因子在动员外周造血干细胞的效果,选择2001/2005收治的20例自体外周血造血干细胞移植患者,男11例,女9例,其中急性髓细胞白血病11例,急性淋巴细胞白血病5例,恶性淋巴瘤3例,多发性骨髓瘤1例。

Methods The expression of CD87 was detected with flow cytometry in 54 AML patients and 20 acute lymphoblastic leukemia patients. The expressionsa of CD87 on U 937, HL-60 and K562 cells were also studied with FCM and reverse transcription-polymerase chain reaction during differentiation induced by phorbol myristate acetate in vitro.

应用流式细胞术检测了54例急性髓性白血病及20例急性淋巴细胞白血病患儿CD87的表达,还用FCM及逆转录-聚合酶链反应法检测了U937、HL-60、K562细胞在氟波酯作用下诱导分化过程中CD87表达的改变。

The number and proliferative responses of T and B lymphocytes to ConA or phorbol myristate acetate,contents of IgG,IgM and IgA in peripheral blood of 14-days-old chickens secondarily infected with E.necatrix were elevated diffe-rent extent compared with that of the control.

结果显示,毒害艾美球虫二次感染雏鸡外周血液T、B淋巴细胞数量及其对ConA或PMA的增殖反应和血清IgG、IgM、IgA免疫球蛋白含量均不同程度地高于未感染毒害艾美球虫的对照组雏鸡。

The present studies were carried out to transfer the Simian Virus 40 Large T antigen gene (SV40LT) into penaeus Chinensis lymphoid cells with recombinant pantropic ritrovirals.

本文采用逆转录病毒介导的方法将细胞转化基因-猿猴病毒大T抗原基因(SV40LT)导入培养的中国对虾淋巴细胞中,尝试对其进行转化培养。

MSCs can be satisfactorily isolated from human marrow with Ficoll Paque reagent and expanded in L DMEM culture medium.

用Ficoll Paque淋巴细胞分离液分离骨髓单核细胞,接种于φ=100 mL/L FCS的L DMEM培养液,分离培养扩增hMSC的效果最好。

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