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Hanson L Breastfeeding provides passive and likely longlasting active immunity Annals of Allergy Asthma and Immunology , 1998,null

杨倩%练高建%黄国庆%陈杰半胱胺对鸡小肠粘膜中分泌型IgA细胞和上皮内淋巴细胞的影响南京农业大学学报, 2002,2

The low dose of sodium arsenite can induce the hormesis of cell survive and MT production.

低剂量砷可促进淋巴细胞的体外存活,并可诱导其MT合成增加。

The influnce of transform ability of macrophage and lymphocyte is not very evident after poured IgY and injected bacterin.

灌喂IgY和注射疫苗对巨噬细胞和淋巴细胞的转化率影响均不很明显。

In contrast, depletion of CD+8 T cells had no effect on the outcome of the disease.

而CD+ 8 T淋巴细胞的排除对此却无明显影响。

Thymus index, milt index and bursa of Fabricius index were measured and lymphocyte proliferation of peripheral blood and milt was detected.

试验期42 d。试验结束后,测定各组胸腺、脾脏和腔上囊系数及外周血和脾脏淋巴细胞的转化率。

Of or relating to lymph or the lymphatic tissue where lymphocytes are formed.

淋巴的,淋巴细胞的淋巴、生成淋巴细胞的淋巴组织的或与之有关的

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性,进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠,3次免疫小鼠后,血清(1:1000)中能检测到较强的MUC1抗体阳性;以最大OD450值之纯化血清做为一抗行蛋白印迹分析,结果显示:纯化血清可识别MUC1蛋白;小鼠脾脏淋巴细胞增殖率检测结果表明:噬菌体展示肽表位可在无佐剂存在情况下,大大促进小鼠的脾淋巴细胞的增殖,证明噬菌体是一较好的生物载体。

The responsibility of spleen lymphocytes from mice immunized for 3 weeks was better than that from mice immunized for 1 week.

免疫3周的小鼠脾淋巴细胞的反应性优于免疫1周的小鼠脾淋巴细胞的反应性。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清,进行以下检测:(1)原子吸收法测血清锌浓度;(2)测胸腺、脾脏脏器重量并与大鼠体重比较,计算脏器指数;(3)HE染色对胸腺、脾脏进行病理观察;(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡,并通过图像分析进行比较;(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达;(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达;(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

The results showed that the starved fish had significant higher white-cell counts than the fed fish,the peak value occurring 20 d after starvation.There were higher multiplication and efficiency of transformation in the lymphocytes in the starved fish than those in the fed fis...

结果表明:饥饿后红鳍东方鲀外周血中白细胞的数量明显高于正常投喂组,且在饥饿20 d时白细胞的数量达到最大值;饥饿后脾脏中淋巴细胞的增殖转化能力与转化率比正常投喂组高,饥饿10 d时达到最大值,随着饥饿时间的延长,淋巴细胞的转化率逐渐降低;饥饿后脾脏中巨噬细胞的吞噬活性与吞噬率均高于正常投喂组,且在饥饿20 d时达到最大值。

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