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Objective:To study the effects of xylene on chromosome aberration of peripheral lymphocytes in workers.

目的:研究二甲苯对实验人员外周血淋巴细胞染色体畸变的影响。

Objective:To investigate the role of purinergic P2z receptors in human leukemic lymphocyte apoptosis induced by extracellular adenosine triphosphate.

目的:研究嘌呤能P2z受体在介导慢性淋巴细胞白血病细胞凋亡中的作用。

Objective To investigate the expression of focal adhesion kinase in peripheral blood of colorectal cancer and its clinical significance.

目的 探讨大肠癌患者外周血淋巴细胞粘着斑激酶的表达及其临床意义。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型,以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型,进行"长动"的抗肿瘤药效学研究:用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究;用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究;用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响;用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Immune function is a essential activity of white blood cells. It was puzzled for the diversity and complexity of immune response. Polarized immune response of immune cells was discovered 30 years ago, which facilitates the study on differentiation of lymphocyte. Recently recognition on multifunctional polarized immune response of lymphocyte and monocyte/macrophage would promote to elucidate the regulatory network of immune cells, diversity and complexity of immune response as well as the study on hemopoiesis.

免疫功能是白细胞的主要功能,免疫反应的多样性和复杂性令人困惑。30年前发现的免疫细胞的功能性极化促进了对淋巴细胞分化的研究,近年来对淋巴细胞、单核/巨噬细胞功能多极化的研究为阐明免疫细胞的调控网络奠定了基础,为阐明免疫反应的多样性和复杂性提供了线索,也为深入研究造血细胞的分化拓展了思路。

The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.

结果表明:在给予不同光照时间的条件下,随着光照时间的延长,褪黑素的分泌水平受到抑制,导致具有特异免疫功能的淋巴细胞百分率下降,具有非特异免疫功能的中性粒细胞百分率上升,经统计分析表明不同组间的测定结果均存在显着差异;采用24h全天光照,用以模拟机体切除松果腺的情况,再给予一定剂量外源性褪黑素,导致机体内具有特异免疫功能的淋巴细胞百分率上升,具有非特异免疫功能的中性粒细胞百分率下降,经统计分析表明不同组间的测定结果均存在显著差异,并且高剂量褪黑素对机体的影响更为明显。

Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatoeytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal interstitial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis oorium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminfferous tubule of the testis,the lymphocytes in the spleen and thymus.

结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞,血液细胞中的淋巴细胞、单核细胞,气管透明软骨细胞,胰腺的腺细胞、腮腺、颔下腺上皮细胞,肝细胞、肝窦壁的内皮细胞,小肠黏膜杯状细胞,心肌细胞,肾间质的纤维母细胞,肺泡上皮细胞,大脑胶质细胞,皮肤真皮纤维母细胞,睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子,脾脏以及胸腺的淋巴细胞等,均有弹力蛋白酶mRNA的表达。

To study the function of thyroid hormone receptors in osteoblasts and in bone, the nuclear extracts of TRs from human osteoblast-like cell line MG63 and rat calvaria were achieved, and the two main kinetic parameters of TRs, affinity constant and maximal binding capacity, were determined by radio-ligand binding assay and Scatchard plot.

其中MG63和骨组织中TRs的Ka值与文献报告的人类外周血淋巴细胞以及大鼠脑组织和肝脏中TRs的Ka值相比,差别显著,但与文献中其他成骨细胞系的Ka值相近,提示成骨细胞或骨组织中的TRs与THs的结合能力有可能确实低于淋巴细胞、脑组织或肝脏,从而使得THs在骨组织中的作用机制及效果可能有别于其他组织。

Result: With a high cancer-inhibiting rate on the animal model having been transplanted the cancer, this medicine can remarkably restrain the growth of cancer cells cultivated in vitro and improve the life quality of mouse model, and also shows obvious promotion and enhancement function on the DTH ear-swelling of mouse with cancer, the proliferation of mouse spleen lymphocyte induced by ConA and the cytokine (TNF-α、IFN-γ) of mouse with cancer respectively. It can obviously decrease the adverse effects like weight reduction, damage of viscera and degradation of life quality caused by the chemotherapeutic medicines, and has a remarkable stimulation on the lymphocyte proliferation induced by ConA. It is also able to improve the ratio of SMMC-7721 cells in G0-G1 period and reduce the cell amount in G2/M period to cut down the possibility of stepping into the division period; additionally, it can obviously inhibit some cancer genes of the cancer cells and meanwhile up regulate the cancer-inhibiting gene.

结果:本品能明显抑制体外培养肿瘤细胞的生长,对在体肿瘤移植模型动物具有较高的抑瘤率,能明显提高模型小鼠的生存质量;对荷瘤小鼠DTH耳肿胀、ConA诱导的小鼠脾淋巴细胞增殖、荷瘤小鼠细胞因子(TNF-α、IFN-γ)呈现明显的促进或提高作用;能显著减少化疗药所引起的小鼠体重降低、脏器损害以及生存质量下降等不良作用;本品含药血清对ConA诱导的淋巴细胞增殖具有明显的促进作用;能提高SMMC-7721细胞G0-G1期比例,降低G2/M期细胞数目,而减少其进入分裂期;对肿瘤细胞的多个癌基因具有明显的抑制作用,同时上调抑癌基因等。

In addition, hindgut has also been analysed, as studies have suggested that numerous lymphoid cells occur at this location in the lamina propria and the submucosa. The Ig-bearing cells are also found in the epithelial layer occasionally.

研究牙鲆黏膜样淋巴组织结果显示在牙鲆的后肠中存在大量产生免疫球蛋白的淋巴细胞,主要分布于后肠的固有层和粘膜下层中,在粘膜层和粘膜层表面也偶有极少量的产生免疫球蛋白的淋巴细胞出现。

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