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Methylation of CpG islands in the promoters induces gene silencing. The multiple tumor suppressor gene P16, located at chromosome 9p21, regulating normal proliferation of cells with a functional unit constituting of p16, cyclin D1 and pRb together. Methylation of P16 gene has been detected in several lymphocytic and plasmacytic malignancies such as lymphoma, acute lymphocytic leukemia and multiple myeloma and shows relationships with the pathogenesis of these diseases. Application of demethylation agents or arsenical to refresh the gene functions will be expected to be a new treatment for hemopoietic malignancies.

基因启动子区CpG岛甲基化常导致基因沉默。P16基因是一定位于9p21的多种肿瘤抑制基因,通过pl6INK4Acyclin D1-PRb通路维持机体细胞的有序增殖。P16基因甲基化在淋巴瘤、急性淋巴细胞性白血病、多发性骨髓瘤等多种淋巴细胞浆细胞肿瘤中被检测到,并与疾病的发生、发展存在一定关系,应用甲基化抑制因子或砷剂去甲基化治疗,恢复基因功能可望成为血液恶性肿瘤治疗的一种新手段。

After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d,然后分为两组,实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞,对照组2除加入HO9810外,还加入了无关细胞,用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

Methods 40 healthy adult mice were randomly divided into control group, CTX immune-suppressed group, Fb treated group, Fb combined with CTX treated group. The influences of Fb in NK cell activity were determined by the method of NK mediated cytotoxicity test;the multiplicative capacity of lymphocyte was assayed by lymphocyte transformation rate of T cell,and the mice spleen T lymphoblast multiplication analytical method was used to measure the IL-2 level.

健康成年小鼠40只,随机分为对照组、环磷酰胺免疫抑制组、黄蘑碱溶性多糖活性组分正常小鼠给药组和Fb环磷酰胺伍用给药组,采用NK细胞介导的细胞毒试验测定自然杀伤细胞的活性,淋巴细胞转化试验测定T淋巴细胞增殖功能,鼠脾T淋巴母细胞增殖分析法测定白细胞介素2(IL-2)的活性,观察Fb对各组小鼠免疫指标的影响。

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞,并在有或无美洲商陆有丝分裂原存在的情况下培养10天后,收集上清液,采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

Meanwhile, the antibodies of VIP enhanced the activation of lymphocytes and the expression of CD68, SYP in sacculus rotundus.

采用抗SP抗体使SP作用受到抑制后,圆小囊组织中淋巴细胞活化分子、DNES细胞表达SYP和巨噬细胞表达CD68分子的量均出现一定程度的减少;而当VIP作用受到抑制后,圆小囊组织中淋巴细胞活化分子、DNES细胞表达SYP和巨噬细胞表达CD68分子的量均出现一定程度的增多。

Objective To study the relationship between the apoptosis of lymphocytes and down-regulation of immunity in patients with uremia by examining the influence of serum of patients with uremia on apoptosis and expressions of Fas in cultured peripheral blood lymphocytes of healthy adult and the interventional function of VitE.

目的 通过观察尿毒症患者血清对培养的正常人淋巴细胞凋亡、Fas分子表达的影响以及VitE的干预作用,探讨淋巴细胞凋亡与尿毒症患者免疫功能低下的关系,并寻找防治对策。

Objective To investigate the effects of aloe-emodin on PHA-induced proliferation and intracellular Ca2 mobilization of T lymphocytes.Methods The proliferation of T cell was tested by MTT assay.

目的研究芦荟大黄素对植物血凝素引起的大鼠T淋巴细胞增殖和细胞内Ca2 浓度([Ca2 ]i)变化的影响,探讨芦荟大黄素对T淋巴细胞增殖的作用和机制。

The results display that the thymus-index, spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 in the experiment groups were significantly higher than those of the control group (P.05); the spleen's lymphocytes translation stimulate indexes in the experiment groups were higher than that of the control group at a significance level(P.01). The peritoneal macrophages can enhance the ability of swallowing. The ability of licking up neutral red was depended upon the dosage of drug.

与对照组相比,试验组的胸腺指数、脾脏指数、免疫球蛋白和补体C3、C4都升高且显著水平为差异显著(P.05);脾脏淋巴细胞转移刺激指数均极显著地高于对照组(P.01), 75μg/mL组的脾脏淋巴细胞转移刺激指数达最高;腹腔巨噬细胞具有一定的吞噬能力,吞噬中性红能力与剂量成依赖关系。

The test groups were drenched orally with different dosages of compound Radix scutellariae granules. After being drenched orally for ten days, their thymus-index and spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 of serum were defermined, and experiments in vivo were done. The mice-spleen's lymphocytes which were cultured in vivo were added with different concentrations of compound Radix scutellariae granules. The lymphocytes translation stimulate indexes were measured. The ability of licking up neutral red of the peritoneal macrophages cultured in vivo was measured in the groups.

应用复方黄芩颗粒给小鼠灌胃10d后,分别测定对照组与实验组胸腺指数、脾脏指数和血清中的免疫球蛋白IgA、IgG、IgM和补体C3、C4;并且进行体外实验,用不同浓度的复方黄芩颗粒剂对小鼠脾脏淋巴细胞进行体外培养,测定淋巴细胞转移刺激指数;在体外培养测定细胞吞噬中性红的能力。

ALA-PDT alleviated GVHD following allo-BMT and preserved GVL in ALL mice One-way mixed lymphocytes predisposed with ALA-PDT and marrow cells from the donors were infused into ALL mice. The body weight increase, haematogenesis recovery and survival time were better then all the other groups.

ALA-PDT减轻急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)小鼠同种异基因骨髓移植后GVHD反应,并且保留了GVL效应将经过ALA-PDT处理过的单向混合淋巴细胞联合供鼠的骨髓细胞输注给ALL小鼠体内,ALL受鼠的体重增长情况、造血功能恢复情况及无病生存时间等指标明显好于对照组。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。