淋巴细胞的

Luminal membrane stained more than 50%, was defined as AQPS-positive acinus. Randomly selected 100～110 acini to calculated the percentage of AQP5-positive acini.

同时计数切片中的淋巴细胞侵润灶，镜下观察测定组织的切面面积，从而计算出唇腺组织的淋巴细胞侵润灶评分。

Meanwhile we observed the change of symptoms, biopsy, T cell inferior group of serum (CD3、 CD4、CD8)、immunoglobulin (IgG、IgA、IgM) and the alexin (C3、C4), cAMP、 cGMP. CD3、CD4、CD8 are detected by immunomomoclonal antibodies APPAP; IgG、IgA、IgM are tested by immunoturbidimetry assay; the content of cAMP and cGMP in blood plasm are tested by radioimmunoassay.

所有观察病例于治疗前均作血、尿、大便常规化验；心、肝、肾功能检查；阴道分泌物检查包括念珠菌、滴虫、阴道清洁度；尿糖、血糖检查；分别观察中药组和西药组治疗前后相关症状及体征变化情况，活体组织病理检查的变化情况；血清T淋巴细胞亚群（CD3、CD4、CD8）和免疫球蛋白(IgG、IgA、IgM)以及补体（C3、C4）各项指标的变化情况；血中cAMP、cGMP含量变化情况，T淋巴细胞亚群水平测定采用单克隆抗体APAAP桥联酶标技术，血清免疫球蛋白IgG、IgA、IgM及补体C3、C4的测定采用免疫透射比浊法。

The kinetics of T-cell subsets, blastogenic responses and anti-NDV serum IgG antibody of La Sota-immunized chicken were investigated using FACS, blastogenesis microassay and indirect ELISA after the chickens were thymusectomized.

然后，在不同的日龄分别用FACS、MTT及间接ELISA测定脾脏和外周血中CD4+和CD8+T淋巴细胞亚类数量、脾T淋巴细胞转化功能及病毒特异的血清IgG抗体的动态变化。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

After curative gastrectomies, the expression level of CCR7 was increased and correlated with the TNM stage (P＜0.05). Conclusions The expression of CCR7 is down??

CCR7在胃癌患者外周血淋巴细胞中的表达与临床病理分期密切相关，Ⅰ、Ⅱ期患者外周血淋巴细胞中CCR7的表达率明显高于Ⅲ、Ⅳ期胃癌患者（P＜0.05）。

Methods:Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain, cell ELISA, immunocytochemistry techniques to investigate the effect of berberine on lymphocyte-endothelium adhesion and the molecular mechanism.

以小檗碱处理人淋巴细胞或人脐静脉内皮细胞后，用蛋白染料染色法研究对淋巴细胞与内皮细胞粘附的作用，用细胞ELISA、免疫细胞化学染色法研究对细胞表面粘附分子表达的影响。

ObjectiveTo probe into the hyperplasy effect of different curcumine administered approaches for the splenic lymphocyte of mice.

目的探讨姜黄素3种不同给药途径对小鼠脾淋巴细胞增殖的影响。方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。

The rats were killed by cutting theirnecks and physical blood was taken for preparing serum, interleukin 2 (IL-2) was detected by radioimmunoassay ; the spleen was taken for measuring ConA-induced reaction of lympho- cyte proliferation and induced production of IL-2; and the activity of induced production of spleen's IL-2 was determined by the means of cell proliferation of mouse thymus; the skin and trachea were taten for slice with paraffin wax, dyeing with methybenzoamine blue so as to survey mast cells .

将大鼠断头留取躯干血制备血清，用RIA法检测白细胞介素2（IL-2）；取脾脏行ConA诱导的淋巴细胞增殖反应测定和IL-2诱生；并用小鼠胸腺细胞增殖法检测脾IL-2诱生活性；取皮肤和气管做石蜡切片、甲苯胺兰染色检测肥大细胞，结果显示：应激组较对照组脾淋巴细胞增殖降低，cpm分别为9466±3.17和24019±3879（P.001）；MC明显减少，分别为8.6±3.0/HP和25.4±3.7/HP（P.001），前组形态异常较其甚（MC多较小，呈多角或不规则状），且组织中可见分散的崩解颗粒。

This experiment unified flow cytometry, the monoclonal antibody technology, the cell fluorescence chemistry technology and unilized the union principle of antigen and antibody to examine T lymphocyte subgroup, the B lymphocyte change in circumference blood of the mice through the flow cytometry; At the same time, the agarose gelatin electrophoresis examined bovine colostrum effect on programmed cell death of thymus.

本实验正是将流式细胞术、单克隆抗体技术、细胞荧光化学技术相结合，并利用抗原抗体结合原理，采用流式细胞仪检测了小鼠外周血的T淋巴细胞亚群和B淋巴细胞；同时采用琼脂糖凝胶电泳技术检测了牛初乳对小鼠胸腺细胞凋亡的影响。

Objective To investigate the relationship between apoptosis of peripheral blood lymphocytes and impaired immunity by assessing the apoptosis and expression of Fas and Bcl-2 in PBLCs of patients with secondary syphilis.

目的检测二期梅毒患者外周血淋巴细胞凋亡及其Fas、Bcl-2的表达，探讨二期梅毒患者免疫功能异常与淋巴细胞凋亡的关系。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。