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BACKGROUND: Alloimmune lung injury, characterized by perivascular lymphocytic inflammation, lymphocytic bronchiolitis, and obliterative bronchiolitis, causes substantial morbidity and mortality after lung transplantation and bone marrow transplantation, but little is known regarding its pathogenesis.

背景:同种免疫肺损伤以管周淋巴细胞浸润、淋巴细胞性细支气管炎和闭塞性细支气管炎为特征,它在肺移植和骨髓移植患者中有较高的患病率和死亡率,但是其病因学目前知之甚少。

Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡,将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养,同时设计不同类型肿瘤细胞(U937及培养胶质瘤细胞)作对照,分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡,将DCs、T淋巴细胞和凋亡胆管癌细胞共培养,同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照,7d后,分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

The level of ifn γ secreted from splenocytes of immunized mice after specific antigen stimulation was detected by a cytopathic effect reduction assay and the splenocytic proliferation of immunized mice in response to antigen restimulation was detected by mtt assay.

分离免疫小鼠的脾淋巴细胞,体外用抗原再刺激,mtt方法检测ifn γ产生水平和mtt法检测淋巴细胞增殖,并对脾脏细菌负荷计数。

Objective:To study the changes in surface markers( CD3+, CD4+, CD8+), lymphocyte transformation rate and the C-fos expression in peripheral blood of schizophreniac before and after treatment.

目的:探讨精神分裂症患者治疗前、后外周血淋巴细胞表面标志(CD3+、CD4+、CD8+)和淋巴细胞转化率及核Fos蛋白表达的变化。

Result: Wallerian degeneration was observed in the proximal and distal ends of all grafts of nerves harvested at 2 weeks post-engraftment.There were so many T lymphocyte in group A. 3 and 5 WEEK group is less.none can be seen in 7 and 9 WEEK group.

结果:术后2周,所有移植段的近端、远端均有不同程度Wallerian变性,A组移植段见明显T淋巴细胞浸润,3周,5周组次之,9周及7周组和自体组未见T淋巴细胞。

The NPC tissue was all EBV-positive except for one sample, which was identified as keratinizing carcinoma, but the control samples were all negative for EBV infection, which was correlated with CD25 expression.

对照组选取鼻咽慢性炎症组织。结果 CD25淋巴细胞在鼻咽癌组织中比在鼻咽慢性炎症组织中的表达明显升高,差异具有显著性意义(P.001),且在未分化癌中表达高于角化型癌和非角化型癌(P.05)。原位杂交显示EB病毒与CD25淋巴细胞高表达有关。

Within three years following-up, 5 patients with NHL were survival, but one case of NHL died at the 2 months after auto-PBSCT, one patient suicided. From 4 cases received allo-PBSCT, one patiant with NHL was died at 79 days later, one patient with chronic lymphoblastic leukemia was surviving, another 2 cases of acute lymphoblastic leukemia were dead at 17 months and 54 days respectively after allo-PBSCT.

随访3年,5例NHL患者无病生存,1例NHL患者于移植后2个月死亡,1例自杀;4例接受异基因造血干细胞移植的患者中,1例非霍奇金淋巴瘤移植后79天死亡,1例慢性淋巴细胞白血病患者无病生存,2例急性淋巴细胞白血病患者分别于移植后第54天、第17个月死亡。

Obstructive jaundice was induced in the mice in the experimental group by common bile duct ligation. The level of the common bile duct diameter, WBC, LYM MID, LYM%, MID% and ALT, AST, TBIL, DBIL, IBIL, ALP and CHOL were measured 12 h and 1, 2, 3, 4, 5, and 7 days after the ligation.

于实验的第12 h,1、2、3、4、5、7 d动态观察胆总管直径及白细胞总数、淋巴细胞总数、中间细胞总数及淋巴细胞总数百分数和中间细胞总数百分数,测血清总胆固醇、丙氨酸氨基转氨酶、天冬氨酸氨基转氨酶、碱性磷酸酶、总胆红素、直接胆红素及间接胆红素值。

Results and ConclusionThe study of time-efficacy relation expresses that after infusing the solution of Phyuanthus emblica Phyllanthus emblica, medicated serum in different time activates the spleen lymphocyte of small mice differently after the 30min but before 180min. During this period, on 2h the activation of the medicated serum is the most obvious. The quantity-efficacy relation research expresses that megadose promotes the propagate of the spleen lymphocyte of small mouse and activates the macrophage in the abdominal cavity more obviously. On the other hand, the small dosage influents the survival rate of the mouse S180 ascites tumor cell more obviously.

结果与结论时效关系研究表明,用余甘子冻干粉溶液灌胃后30~180 min内不同时相含药血清对小鼠脾淋巴细胞具有活化作用,并在给药2 h时实验血清活化作用明显;量效关系研究表明,余甘子大剂量组实验血清对促进小鼠脾淋巴细胞增殖,促进小鼠腹腔巨噬细胞细胞活化作用以及小剂量组实验血清对小鼠S180腹水肿瘤细胞存活率的影响均具显著差异性。

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If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。