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Results (1) Maximum bound volume and equilibrium dissociation constant of β2AR of lymphocyte in asthma patients at remission stage were markedly higher than that in normal subjects, while cAMP levels in blood plasma showed no difference.

结果缓解期哮喘患者外周淋巴细胞βAR的密度较正常人明显升高,血浆cAMP水平无显著差异,而发作期哮喘患者βAR及血浆cAMP水平均较正常人明显降低;缓解期哮喘患者外周淋巴细胞β2ARmRNA水平与正常人无显著差异。

Objective In order to explore the effect of intracellular signal transduction on apoptosis of cord blood mononuclear cells.

目的 探讨新生儿T淋巴细胞内信号传递与脐血淋巴细胞凋亡的关系。

in order to probe into whether glycine supplementation plays an adjusting role in sports performance and immune functions, the authors divided 30 mice randomly into an exhaustive exercising group (e1), glycine supplemented exhaustive exercising group (e2) and a normal resting control group, with 10 mice in each group, supplemented glycine to mice in group e2 and physiological saline to mice in the other two groups, let mice in groups e1 and e2 do an exhaustive swimming exercise 4 weeks later, applied the trace ch50 hemolysis method to analyze supplement activity, applied the mtt method to perform a quantitative analysis on the lymphopoiesis ability of spleen, and thus revealed the following findings: compared with mice in group e1, the time taken by mice in group e2 to swim to an exhausted state is significantly longer (p.05); total supplement activity in blood serum of mice in group e1 is significantly lower than the same of mice in group s (p<0.01), and total supplement activity in blood serum of mice in group e2 is significantly higher than the same of mice in group e1 (p.01), but still significantly lower than the same of mice in group s (p.05); as observed from the test of the lymphopoiesis ability of spleen, the lymphopoiesis ability of spleen of mice in group e1 is significantly lower than the same of mice in group s, and the lymphopoiesis ability of spleen of mice in group e2 is significantly higher than the same of mice in group e1, but still significantly lower than the same of mice in group s.

四型肽精氨酸脱氨酶在类风湿性关节炎。。。以马来酸酐与氨水为原料热缩聚法合成。。。摘要:为探讨甘氨酸补充对运动能力及免疫功能是否具有调节作用,将30只小鼠随机分成力竭游泳运动组(e1)、甘氨酸补充后力竭游泳组(e2)和静息正常对照组,每组10只。在e2组补充甘氨酸,其余两组给予生理盐水,处理4周后,e1组和e2组均做力竭性游泳运动。采用微量ch50溶血法分析补体活性,采用mtt法定量分析脾脏淋巴细胞增殖能力。结果显示,与e1组比较,e2组游泳至力竭的时间显著延长(p<0.01)。e1组血清总补体活性显著低于s组(p<0.01),e2组显著高于e1组(p.01),但仍显著低于s组(p.05)。在脾脏淋巴细胞增殖试验中观察到,e1组显著低于s组,e2组显著高于e1组但仍显著低于s组。

Phylogenetic analysis of the long terminal repeatand env gene of htlv-1 gave a phylogenetic classification of htlv-1 genotypes into five major molecular subtypes: cosmopolitan, japanese, west african, central african, and melanesian. in order to understand the major genotype of htlv-1 prevalent in china, htlv-1 env gene was amplified by pcr and sequenced from peripheral blood lymphocyts of three htlv-1 carriers in the putian region of fujian province.

根据基因组及血清学反应可将其分为人t淋巴细胞白血病病毒1型(htlv-1)和2型(htlv-2),两型病毒基因组同源性大于60%。htlv-1主要侵染t淋巴细胞,可引起成人t细胞白血病、热带下肢痉挛性瘫痪或htlv-1相关脊髓病,并且在多种自身免疫病患者体内也发现了该病毒的存在。

Objective To investigate the effect of nidus vespae on lymphocyte blastisation in mixed culture system of lymphocyte and pancreatic islet.

目的 初步探索露蜂房对淋巴细胞与胰岛混合培养系统中淋巴细胞转化的影响。

Igniarius were carried out. It will be of scientific significance in researching and developing rare medicial fungi and be useful for searching new natural antitumor medicine.The crude polysaccharides of P. igniarius PIP30、PIP60、PIP80 and PIWE1-10、PIWE10-100、PIWE100 were obtained by ethanol precipitation and ultrafiltrate respectively after hot water extracting.

首先对桑黄子实体多糖进行了分离纯化,通过热水提取、乙醇分级沉淀分离得到粗多糖PIP30、PIP60和PIP80,通过超滤分离得到粗多糖PIWE1-10、PIWE10-100和PIWE100;6种粗多糖经体外刺激淋巴细胞增殖实验表明均有体外刺激淋巴细胞增殖的作用。

Tumor cells grew actively in the tumor tissues of the control group. Prodrug therapy group: small amount of tumor cells were denatured vacuously,others were infiltrated by lympholeukocyte,and the growth of tumor cells were surspressed.Prodrug themochemotherapy group: what we can see that tumor cells were denatured vacuously, mesoplasts crinkled, cellular boundary disappered, only a small number of tumor cells remained, fibroblast were seen scatteredly, tumor cells were invaded by a lot of lympholeukocyte and eosinophilic granulocyte. Normal liver tissues, stomach tissues, lung tissues, pancreas tissues, small intestine tissues, large intestine tissues showed normal shape.

对照组肿瘤组织见肿瘤细胞生长活跃;前药治疗组肿瘤组织见有少量细胞空泡变性,少量淋巴细胞浸润,肿瘤细胞生长受到抑制;前药热疗组肿瘤组织可见肿瘤细胞空泡变性,细胞核皱缩,边集甚至消失,仅残留少量肿瘤细胞,并可见散在的成纤维细胞,大量淋巴细胞和嗜酸性粒细胞浸润;3组裸鼠正常肝组织、胃、肺、胰腺、小肠、大肠组织均呈正常形态学,无病理性损伤改变。

In addition, the influences of BIS on the activities of lymphocytes in vivo and in vitro were studied, including the blast transformation of lymphocytes, the number of acid α-naphthyl acetate esterase positive lymphocyes, the cytostatic effect of hepatic LGL against hepatoma cells, in older to provided the most adequate scientific foundation for the best application of hepatoma immunotherapy.

此外还对上述不同免疫制剂在体应用对正常大鼠血淋巴细胞转化率及酸性非特异性酯酶阳性淋巴细胞数量的影响;不同免疫制剂对分离培养的肝大颗粒淋巴细胞抑肝癌细胞增殖的直接影响的研究,为肝癌免疫治疗的更佳选择提供更充分的科学依据。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberines function may be downregulating the expression of ICAM-1 on the HUVEC .

结果:小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附,其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达;小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附,对淋巴细胞表面粘附分子CD18的表达无影响。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberine's function may be downregulating the expression of ICAM-1 on the HUVEC .

结果:小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附,其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达;小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附,对淋巴细胞表面粘附分子CD18的表达无影响。

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