淋巴细胞的

Routine detecting techniquefor cell immunity such as white blood count and differential, lymphocyte blastogenesis test has beenused, and combine with flow cell technology to detect the level of cell immunization after advancedimmunization at several important aspect, and tracking experiments to observe changes in the numberof the WBC and lymphocytes, lymphocyte proliferation, the dynamic changes of CD4~+ and CD8~+lymphocyte subsets andγ-IFN; and detected dynamic changes rules of IgG, IgM in humor andmucosa with indirect ELISA, then compare with ordinary immunization methods.

主要采用白细胞计数和分类计数、淋巴细胞转化试验等常规细胞免疫检测技术结合先进的流式细胞技术从细胞免疫的几个重要方面入手检测猪瘟超前免疫后细胞免疫的水平，并进行跟踪试验，观察其白细胞、淋巴细胞数目变化情况，淋巴细胞增殖情况，CD~(4+)和CD~(8+)淋巴细胞亚群及γ-IFN的动态变化规律；同时通过间接ELISA方法检测体液和黏膜中各IgG、IgM的动态变化规律，同时与普通免疫方法进行比较。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

Significant levels of human immunoglobulin were detected in sera of hu-PBL-SCID mice 4 weeks after engraftment.

在移植后4周，其小鼠血清中存在人免疫球蛋白，其脾淋巴细胞中，所检测的8 种免疫表型人淋巴细胞亚群均存在，表明人淋巴细胞在SCID小鼠体内出现了增殖或重建。

Methods The DNA injury was determined with single cell gel electrophoresis, the contents of NO and NOS were measured by NO and NOS kits respectively and apoptosis of lymphocytes was determined by flow cytometry and gelose electrophoresis.

建立硫芥中毒淋巴细胞模型，分别用单细胞凝胶电泳法检测淋巴细胞DNA损伤；用镀铜镉还原法和一氧化氮合成酶试剂盒检测中毒后淋巴细胞培养上清液一氧化氮和一氧化氮合酶的变化；用流式细胞仪和琼脂糖凝胶电泳法检测细胞凋亡。

We also have observed some organs pathological changes of juvenile Jian Carp, such as pancreas acinous gland and zymogen granules of acinous gland cell disappearing, intercellular substance hyperplasia and inflammatory cells soakage, hepatatrophia, liver cell granular or vacuolar degeneration and necrosis, karyolysis or pyknosis, glycogen granules decreasing, metanephros atrophy, metanephric canaliculus epithelium granular or vacuolar degeneration and necrosis, mitochondrion swelling and mitochondrion cristae disappearing, karyolysis, distal convolutal tubule microvilli desquamating, spleen marrow cell degeneration and necrosis, intercellular substance of spleen hyperplasia, spleen atresia, blood corpuscle disappearing.

后肾土黄色、淡褐色或苍白色，肾小管上皮细胞肿胀、颗粒或水泡变性、坏死，细胞内有大量血细胞流出，线粒体肿胀，嵴结构消失，细胞核溶解，肾间质甲状腺滤泡和拟淋巴细胞增生，远曲小管微绒毛脱落、管道细胞界限不清。心脏肌纤维肿胀、颗粒或空泡变性，严重的肌纤维溶解、变细或断裂，肌纤维间水肿、炎性细胞浸润，部分心肌细胞核浓缩。脾脏暗褐色，脾髓质细胞变性、坏死，拟淋巴细胞明显减少，淋巴细胞岛少见、岛中细胞成份减少，黑素巨噬细胞中心减少、体积缩小，脾脏网状基质水肿，脾窦闭锁，血细胞减少。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

The cancers studied here were throat cancer, aute non- lymphoblas He leukem ia and acut lymphoblastic leukemia. The result s indicated that,(1)the levels of Leu, Lys, Ser Asn,Ile,Gly,and Thr were obviously decreased whea lse the levels of orn, Trp evidently increased in cultural liquid of throat can cer cell line indicating that the growth of HepⅡ cell consumed 7 amino acids and releaced 2 amino acids into cultural liquid.(2)the levels of Glu, Gls, Leu, Phe, Tyr and Trp were evidently increased while the levels of Thr , His, Ala decreased in plasma from patient with ANLL. These r esults were consistent with the previous report.(3)the levels of Gly, Trp and Phe in plasma from patients with ANLL evidently de creased and the levels of Ala, His increased after therapy indicating that cance r cells were growing in an anaerobic catabolism state.

这些癌肿包括喉癌HepⅡ细胞，急性非淋巴细胞白血病和急性淋巴细胞白血病，结果表明：(1)喉癌细胞株培养过程中亮氨酸、赖氨酸、丝氨酸、天冬酰胺、异亮氨酸、甘氨酸以及苏氨酸等水平明显降低，而色氨酸水平明显增加，说明喉癌细胞的生长繁殖必须依赖以上7种氨基酸同时释放了色氨酸；(2)急性非淋巴细胞白血病患者血浆中的谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸等水平明显升高，而苏氨酸、组氨酸、丙氨酸等水平明显降低，这些结果与国际报道相一致；(3)经治疗后，ANLL患者血浆中甘氨酸、色氨酸和苯丙氨酸等水平明显降低，而丙氨酸、组氨酸等水平明显升高，表明肿瘤细胞处在无氧代谢。

The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid.

取小鼠外周血非贴壁的淋巴细胞，与靶细胞混合淋巴细胞培养，进行淋巴细胞增殖和反应性T细胞功能检测，并检测细胞培养液中IL-2、TNF-α和IFN-γ水平。

Methods Intrathymic injection of 2.5×107 Wistar spleen cells in neonatal SD rats was performed followed by mixing lymphocyte culture with Wistar rat spleen cells (20Gy) at 4-6 weeks of age. The levels of IL-4 and IFN-γ were measured by ELISA on the third day.

将2.5×107个Wistar大鼠脾淋巴细胞注入新生SD大鼠胸腺内，4～6周龄时取其脾淋巴细胞与Wistar大鼠脾淋巴细胞(20Gy X射线照射)进行混合培养，于72h测定IL-4和γ干扰素的含量。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。