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ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平;肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定;外周血来源树突状细胞培养和鉴定:将gP96—多肽复合物和DC体外共同孵育制备疫苗,在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

Our experiment is unique to clone porcine IL-4 gene from lymphocytes of the local species of Chenghua porcine and systematically study its expressing effect in vivo on the immune system and immune responses of mouse and pig to trivalent vaccines against Swine fever, the Pasteurellosis and Erysipelas suis and pig paratyphoid vaccine.

将得到的序列在GenBank和EMBL数据库中进行了同源比较,结果表明它与来源于长白猪肠粘膜固有层淋巴细胞的白细胞介素-4有99%同源,与来源于杂交猪T细胞的白介素-4有96%同源,说明猪种及组织细胞来源的不同可能使基因有一定的差异。

The model had a high incidence and its pathologic changes were close to those of multiple sclerosis, so it might be an ideal model for the research of MS. 2. It is supposed that the activity of T cell response to MOG_(35-55) and the increases of anti-MOG_(35-55) antibody and IFN-γlevels may play key roles in the onset of EAE.

通过比较EAE小鼠和正常小鼠T淋巴细胞的增殖、血清抗MOG_(35-55)抗体和IFN-γ水平的不同,显示T细胞的活化、抗体的升高、细胞因子的异常可能共同导致了EAE的发生。

The results of SDS-PAGE showed that the expression products were about 31KD and reached their expression peaks about 2 hours after inducing and the expression quatity of fusion protein had 22.7% ratio of total thalline protein, while the E.

提取并纯化pET-PIL-8的融合表达蛋白并用改进的琼脂扩散法检测其生物学活性,通过检验分析表明所获得的重组猪IL-8具有趋化猪外周血淋巴细胞的作用,具有较强的生物学活性。

A chosen vessel 上帝所选的人 the weaker vessel 较弱的人(指女性 lymph node Any of the small, oval or round bodies, located along the lymphatic vessels, that supply lymphocytes to the bloodstream and remove bacteria and foreign particles from the lymph.

淋巴结任一种长于淋巴管内的小的、呈椭圆或圆形的组织,它为血液输送淋巴细胞并清除淋巴液中的细菌和外部成分Also called: lymph gland filter n。滤纸,过滤器,滤波器 vt。

Objective In order to know the essential meaning of immune disorder in Recurrent Aphthous Ulcer patients and to search the agreeable treatment meanwhile, the cluster of differentiation of peripheral lymph cells were detected and analyzed by flow cytometry, and afterwards those patients were treated by immunomodulator.

目的为了更好的理解RAU患者免疫紊乱的实质及寻找有效的治疗方法,我们采用流式细胞技术,对RAU患者发病期和康复期外周血淋巴细胞的表型进行测定、分析,探索流式细胞技术在RAU的诊断及指导临床治疗方面的作用。

Simvastatin may exert its function to prevent allograftangiopathy by the mechanism of cholesterol-lowering, the increase expression ofeNOS and secretion of NO, direct anti-inflammatory effect, commissural inhibitionwith cyclosporine on MHC-II and CD4~+ T cells.

他汀类药物可能通过降低血胆固醇水平、促进eNOS 的表达并增加NO 的分泌、直接的抗炎作用、与环孢素联合抑制MHC-II 的表达和T 淋巴细胞的活化增殖等机制来对抗同种异体血管硬化的作用。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂,以不同浓度的TMB-8及与环孢菌素A联合作用于该系统,用流式细胞术检测T细胞早期活化标志CD69分子的表达;以活体染料羧基荧光素乙酰乙酸染色流式细胞术,分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数;以碘化丙啶染色分析细胞周期的分布情况。

Baicalin could inhibit the initial-period and mid-period activation of mouse T lymphocytes stimulated by Con A or PDB,which suggests that baicalin have the inhibitory effect on signaling pathway of T lymphocytes activation and be a potential effective immunoinhibitory agent.

结论黄芩苷能够明显抑制多克隆刺激剂Con A或PDB诱导的T细胞早期和中期活化,且呈明显的量效关系。提示黄芩苷在T细胞活化过程的信号转导中有抑制作用,从而可以通过抑制T淋巴细胞的活化,发挥免疫抑制作用,是一种潜在的免疫抑制剂。

The abnormal expressions are not only involved in the development, but also in the lymph nodes metastasis of non-small cell lung carcinomas.

研究表明''-'',CD44蛋白能介导肿瘤细胞与毛细血管内皮细胞结合,在肿瘤细胞的转移中起着重要的作用,CD44蛋白表达阳性的癌细胞可能获得淋巴细胞的&伪装&,容易进人淋巴结,导致转移;CD44蛋白和基因的异常表达与消化、泌尿和生殖等许多系统肿瘤的发展和淋巴结转移密切相关。

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If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。