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淋巴细胞瘤

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Changdong" showed a noticeable enhancive effect when it was co-administrated with other anti-tumor agents or when it was co-applied with radiotherapy. The action mechanism study showed that an apparent cytotoxic effect of "Changdong" on ten tumor strains in vitro with a quantity-effect relationship was observed. Immunity experiment showed that "Changdong" could induce proliferation of spleen lymphocyte in mice bearing tumor and could improve NK cell" s activity, whereas has no impact on IL-2" s reproduction. It was also noticed that "Changdong" could induce human liver cancer QGY" s apoptosis in vitro, and it happened mainly on G0-G1 phase.

结果:"长动"对小鼠移植瘤模型和人体肿瘤裸鼠异种移植均有明显抑制作用,量效关系明显:"长动"与放疗和化疗分别联合应用有明显的增效作用:作用机制研究结果:细胞毒性试验研究表明"长动"在体外对10株肿瘤细胞有明显的细胞毒性作用,量效关系明显;免疫学试验表明"长动"可以诱导荷瘤小鼠的脾淋巴细胞增殖,增强NK细胞的活性,对IL-2的产生无明显影响;"长动"在体外可明显诱导人体肝癌QGY细胞凋亡,使细胞阻滞于G0-G1期。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型,以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型,进行"长动"的抗肿瘤药效学研究:用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究;用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究;用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响;用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Balb/c mice were immunized with the purified PRRSV - BJ isolate. mouse spleen cell were fused with myelomas NSo .culture supernatants of hybridism's were screed by indirect ELISA. Positive colonies were cloned three times by limited dilution. One cell line named 6PR, which could secret McAb stably was obtained.

用纯化的猪繁殖与呼吸系统综合征病毒免疫Balb/c小鼠,应用淋巴细胞杂交瘤技术,取脾细胞与NS0骨髓瘤细胞融合,经间接ELISA筛选,三次有限稀释法克隆,得到一株能稳定分泌抗猪繁殖与呼吸系统综合征病毒单克隆抗体的杂交瘤细胞株:6PR。

Results The lesion appeared to originate in the right atrium and involved the venae cavae and the left atrium.

结果肿物位于右心房并向上下腔静脉延伸、部分与左心房壁相连,镜下为弥漫性增生的异型淋巴细胞,瘤细胞胞体大,胞浆丰富,间有瘤巨细胞。

Methods The mouse models bearing S180 sarcoma were treated with cadmium chloride with dosages of 0.25,1.00 and 4.00 mg.kg-1,the anti-tumor effect was evaluated by calculating of tumor weight;spleen and thymus indexes,carbon phage assay,lymphocyte transformed assay and hematolysis assay were used to measure the effect of cadmium chloride on immune function.

应用0.25、1.00及4.00 mg.kg-1的氯化镉处理S180肉瘤小鼠模型,检测瘤重确定氯化镉对S180肉瘤的抑瘤作用,采用胸腺指数、脾脏指数、碳粒廓清试验、淋巴细胞转化实验、半数溶血素实验检测小鼠免疫功能的变化。

Methods: Mice were inoculated with tumor cells. Inhibitory effect of transfection with pCH510 on murine tumor origined from different inoculative dose and inhibiting effect of immediate transfection pCH510 after chemotherapy on tumor were observed, respectively. By cell culture technique, the influence of chemotherapeutic drug to activation of marcrophages and lymphocytes after i.p. injection of drug was observed. By cell counting method, the kinetics of the change of number of peripheral blood immunocytes induced by administration of chemotherapeutic drug was observed. The inhibitory effect of transfection with pCH510 five days after chemotherapy on murine tumor was observed.

采用瘤细胞接种建立小鼠肿瘤模型:通过基因转染,观察pCH510对不同接种量所形成的小鼠肿瘤的抑制作用以及小鼠肿瘤化疗后立即进行pCH510转染的抑瘤效果;采用细胞培养技术,观察小鼠体内注射化疗药物后,其对小鼠腹腔巨噬细胞和脾淋巴细胞激活功能的影响;采用细胞计数的方法,观察化疗药物所致小鼠腹腔巨噬细胞和外周血免疫细胞数量变化的动力学;另外小鼠肿瘤化疗后第5天进行pCH510转染,观察抑瘤效果。

The expression of Cat V was significantly raised in the epithelial-rich and mixed cell thymomas when compared with that in the lymphocyte-rich ones. No statistically significant difference was found on the variables such as perioperative myasthenic crisis, sex or age of patient, duraton of sickness and expression of Cat V.

上皮细胞型(75.00%)和混合细胞型(85.00%)胸腺瘤中Cat V阳性表达率显著高于淋巴细胞型(21.21%)(P.01),而上皮细胞型与混合细胞型之间差异无统计学意义;良性与恶性胸腺瘤中Cat V阳性表达率差异无统计学意义;男性患者与女性患者之间、35岁以上患者与以下患者之间胸腺瘤中Cat V阳性表达率差异均无统计学意义。

Based on these results and inferred to related reports from other labratories, it was possible to make some analyses and conclusions or inferrences:(1) CD〓AK with tumoricidal activity were induced and expanded in number througth costimulation of PBMC with anti-CD〓 McAb . and r IL-2 ;(2) CD〓AK induced and expanded in such manner did exibit more potent proliferation·ability and cytotoxicity which maintained for lonser time than those of LAK cells, thus CD〓AK was a new variety of antitumor effector cells worth to be explored;(3) CD〓AK could mediate MHC nonrestricted cytotoxicity and kill tumor target cells through inducing necrosis and apoptosis;(4) Normal mature lymphocytes of PBMC could be induced to proliferate and /or to die from apoptosis when they were costimulated by anti-CD〓McAb and rIL-2. Both proliferation and apoptosis were existing in the same cultivation system sugsesting that the presence of rIL- 2 might provide some accessary signals for apoptosis.

以这些结果为基础并参考其它有关文献可能做出如下分析与结论或推论:(1)用抗CD〓单抗和rIL-2共刺激外周血单个核细胞能诱生扩增出具有杀瘤活性的CD〓AK细胞,(2)与LAK相比,用这种方法诱生扩增的CD〓AK增殖能力强、细胞毒活性强而且维持时间长;CD〓AK是一类值得开发的抗瘤效应细胞;(3)CD〓AK能够介导MHC非限制性细胞毒活性,可以通过诱导靶细胞坏死和/或凋亡杀伤肿瘤细胞;(4)正常外周血单个核细胞中的成熟淋巴细胞在受到抗CD〓单抗和rIL-2共同刺激后既可诱导增殖也可诱导凋亡,两者并存于同一体系,推测rIL-2的存在可能为细胞凋亡提供一些辅助信号。

In part Ⅲ of this ariticle,The authors report some other neoplasms and precancerous changes, and they are adenoma of stomach, hemangiofibroma of ovary,carcinoma of Fallopian tube, osteo-petrosis,cholangiocarcinoma, polyps, leukoplakia,atypical hyperplasia, papillar...

这些肿瘤是鼻咽癌、副鼻窦癌、鼻腔癌、食管癌、卵巢癌,淋巴细胞性白血病、白色肉瘸、淋巴肉瘤,外阴癌、纤维肉瘤、原发性肝癌、小肠癌、结肠癌、乳腺癌,圆形细胞肉瘤、纤维瘤、脂肪瘤、马立克氏病、肾癌、畸胎瘤、膀胱乳头状瘤和口腔癌。

Results Only DCs cocultured with apoptotic cells can induce special CTLs, while the group of U937 cells and culture cells can not.

结果 与凋亡胶质瘤细胞共培养之DCs可以有效提呈胶质瘤细胞抗原,有强烈的免疫应答,刺激的细胞毒T淋巴细胞特异性杀伤胶质瘤细胞。

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