淋巴细胞生成

Objective: To investigate the effect of polysacchaide of silkworm pupa on immunological function in mice.

目的：通过测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响，研究蚕蛹多糖对小鼠免疫功能的影响。

MethodsThe immunosuppressive mouse model was established by using hydrocortisone. Huangqi Fuzhengtang and Yupingfeng San apozema were given to the mice intragastrically. The contents of hemolysin, IL-2, and INF-γ in the mouse serum and the expression of CD3+, CD4+, and CD8+ in the peripheral blood lymphocytes were measured.

以氢化可的松造成免疫低下小鼠模型，用黄芪扶正汤和玉屏风散水煮液进行灌胃，检测小鼠血清溶血素的生成、IL-2和INF-γ的含量以及外周血淋巴细胞CD3+，CD4+和CD8+的表达。

Results 6.3 mg/kg diazinon decreased the number of IgMplaque forming cell,blood serum erythrocytolysin level and DTH to SRBC.12.5,25 mg/kg dizainon could decrease the relative weight of thymus at about 20%,the number of IgMplaque forming cell at about 39% and 52%,the blood serum erythrocytolysin level at about 15% and 20%,the DTH to SRBC at about 33% and 35%,the ability of T lymphocyte proliferation at about 27% and 35%,the activity of NK cell at about 24% and 28%,respectively.

结果 敌匹硫磷染毒4周后，6.3 mg／kg组小鼠的体液免疫功能和迟发型变态反应受到抑制；12.5，25 mg/kg组小鼠的胸腺脏器系数均降低20％，抗体生成细胞数分别降低39%和52％，血清溶血素水平分别降低15％和20％，迟发型变态反应水平分别降低33％和35％，淋巴细胞增殖能力分别下降27％和35％，NK细胞活性分别下降24％和28％。

The immune function was evaluated with T lymphocyte proliferation,NK cell activity assay,IgMplaque forming cell assay,blood serum erythrocytolysin assay and delayedtypehypersensitivity to SRBC.

采用T淋巴细胞增殖和迟发型变态反应来评价细胞免疫功能，采用血清溶血素测定和抗体生成细胞检测来评价体液免疫功能，采用乳酸脱氢酶法测定自然杀伤细胞活性来评价非特异性免疫功能。

The results show that Yiliqi can enhance the delayed type hepersensitivity of mouse and phagocytosis of macrophage,and it can also increase the positive rate of peripheral T cell containing acid α-naphthyl acetate esterase and the number of antibody forming cell in spleen.

实验结果表明，小鼠连续20天给与该受试物，在人使用量的1倍、10倍、30倍剂量组能明显增强小鼠迟发型变态反应及增加外周血T淋巴细胞ANAE阳性率，并可提高小鼠巨噬细胞吞噬功能和脾脏抗体生成细胞数。

They function in the immune response through a variety of actions, including stimulating the production of nonsensitized lymphocytes and activating macrophages.

抗'。''原激活的各种物质，他们通过多种行为对免疫反应起作用，包括刺激不灵敏淋巴细胞的生成和激活巨噬细胞

Thymosin 1（Tα1） is a acidic protein composed of 28 amino acid residues with isoelectric point 4.2 and molecular weight 3102.Its amino acid sequence is: N-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu -Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-C. T1 is a T-cell potentiating factor.

胸腺素α1（thymosin 1，Tα1）是由28个氨基酸组成的酸性多肽，等电点4.2，相对分子量3108，其氨基酸序列为：N-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-C[1，2]。Tα1具有多种免疫活性，可以促进T淋巴细胞的成熟和分化，促进成熟的T细胞、自然杀伤细胞分泌各种细胞因子如IL-2、INF-α等，同时也促使高亲和力白介素-2受体的生成[3-10]。

After killing the mice, weights of thymuses and spleens, and the macrophage phagocytosis were detected. using sheep red blood cells as antigen, the amounts of antibody-producing cells in mice were counted. through lymphocyte conversion test, lymphocyte conversion ratios of spleen lymphocytes stimulated by cona in mice were determined.

处死后检测小鼠胸腺重、脾重、巨噬细胞吞噬功能；采用绵羊红细胞作为抗原，检测小鼠抗体生成细胞功能；采用mtt法检测小鼠脾淋巴细胞对cona刺激的转化能力。

Prove that the soap glucoside of ginsengs is a kind of very good immunity strengthens the pharmaceutical through animals experiment, It is strengthening the systematic function of single-nuclear macrophage cell, promotes the formulation antibody and lymphocyte transformed, Promote some lymph secretion of factor, play a regulating role in natural killer cell-interferon-IL-2 regulate network, and protect the organization cell attacked by the virus.

通过动物实验证明人参皂甙是一种很好的免疫增强剂，它具有扶正固本、增强单核巨噬细胞系统功能，促进特异性抗体的生成和淋巴细胞转化，促进某些淋巴因子的分泌，对天然杀伤细胞-干扰素-白介素-2调节网起正调节作用，并能保护受病毒侵袭的组织细胞。

Results: Bushen Huoxue prescription can dose dependent increase T-lymphocyte dyeing rate, carbon particle clearance index number, haemolysin antibody formation and delayed type hypersensitivity on deficiency of kidney-Yin Jimpy mice.

结果：补肾活血方呈剂量依赖性增加肾阴虚小鼠的T淋巴细胞染色率、增加碳粒廓清指数、增强溶血素抗体生成及增强迟发型超敏反应。

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我尽力帮你找人帮忙。

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起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。