淋巴细胞

Skin stimulation test and implantation in vivo found no abscess and fistule formation. Histological section showed there was moderate inflammatory reaction of forign object exciation at the early stage of implantation. At the intermediate stage, inflammatory cells decreased, mainly including lymphocytes, and there was little inflammatory reaction and antigen reaction. Meanwhile, SIS degraded sequently. At 12 weeks, SIS was almost completely degraded and absorbed.

皮肤刺激试验和体内植入研究发现，无脓肿及瘘管形成，组织切片发现SIS植入后组织反应在早期呈现出异物刺激引起轻中度炎性反应，中期时炎症细胞减少，以淋巴细胞为主，炎症反应和抗原反应都较小，同时进行程序性的降解。12周时SIS几乎完全降解吸收。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

Part II Regulation of gambogic acid on anti-apoptotic protein of Jurkat cells and Raji cellsTo study the Regulation of gambogic acid on anti-apoptotic protein of Jurkat cells and Raji cells.

第二部分藤黄酸对淋系肿瘤细胞系的细胞凋亡及抗凋亡蛋白的调控作用研究藤黄酸对人急性T淋巴细胞白血病细胞株Jurkat细胞和人Burkitt淋巴瘤细胞株Raji细胞的诱导凋亡及抗凋亡蛋白的调控作用。

Part III Gambogic acid induces death inducer-obliterator 1-mediated apoptosisTo explore the apoptosis inducing effects of GA on Jurkat cells and Raji cells and its underlying mechanism.

第三部分藤黄酸对淋系肿瘤细胞系死亡诱导清理子的调控作用探讨藤黄酸对人急性T淋巴细胞白血病细胞株Jurkat细胞和人Burkitt淋巴瘤细胞株Raji细胞的抗癌作用的分子机理。

Materials and Methods: Campare gasless hand-assisted laparoscopic surgery, carbon dioxide pneumoperitoneum laparoscopic surgery and operation on Cholecystectomy, colorectal resections, according to the study of operation time, expense in hospital, number of days, pathologic type, extension of radical cure, and analysis of the blood lymphocytes, immune function of erythrocyte, we could ascertain the application value of gasless hand-assisted laparoscopic surgery.

材料与方法：对比研究非气腹手助腹腔镜手术、CO_2气腹腹腔镜手术及开腹手术，从早期的胆囊切除手术的临床应用过渡到结直肠癌根治手术，分别从手术时间、住院费用、住院天数、病理分型、根治范围等进行对比研究，然后从人体外周血淋巴细胞、红细胞等免疫功能方面作进一步分析，以确定非气腹手助腹腔镜手术在临床中的应用价值。

Penetrate into the affections of human immune functions by above three kinds of cure pattern, gasless hand-assisted laparoscopic surgery had obvious difference with the others on blood lymphocytes, immune function of erythrocytes RC3bRR, RICR, RFER, RFIR, IL-6、C-reactive protein and so on.

深入研究三种术式对人体免疫功能的影响，非气腹手助腹腔镜手术在外周血T淋巴细胞亚群数量和分类的变化、RC3bRR(红细胞Ⅰ型补体受体花环率)和RICR、RFER和RFIR、血浆IL-6、CRP等急性反应指标含量分析均有明显差异。

Compared with acute-phase reaction and erythrocytic immune function, neutrophilic counts, interleukin-6, natural killer cells, C-reaction protein between gasless hand-assisted laparoscopic surgery and carbon dioxide pneumoperitoneum laparoscopic surgery, we found there were obvious difference on N/L rate, CRP, IL-6, Which provided evidence for evaluate the gasless hand-assisted laparoscopic technique superiority to the traditional surgery, and treatment for clinical immune modulation.

通过非气腹手助腹腔镜、CO2气腹腹腔镜和开腹手术创伤后出现的急性期反应和红细胞免疫功能的检测，中性粒细胞计数、IL-6，NK，CRP浓度等APR指标检测，发现非气腹手助腹腔镜结直肠癌手术术后中性粒细胞／淋巴细胞、CRP、IL-6等免疫指标的变化情况较腹腔镜组、开腹组有明显区别，为评价此手术技术是否较传统方法优越和临床免疫调节治疗提供依据。

Twenty five patients with gastritic carcinoma were vaccined with dendritic cells pulsed with tumor antigen and T cell subsets of peripheral blood, Th1 type and Th2 type (IL-4) cytokines of these patients serum were detected at different time point.

第三部分，从外周血诱导制备DCs疫苗，皮下注射治疗25例胃癌患者，初步观察DCs疫苗治疗前后胃癌患者迟发性超敏反应，T淋巴细胞亚群，NK细胞，外周血清IFN-γ、IL-4的水平及外周血PBMC在自体肿瘤抗原刺激下分泌IFN-γ、IL-4的能力。

Methods The inhibitory effects of Taurine (50 mmol·L-1) on glycolaldehyde (0.1 mmol·L-1) induced DNA damage in spleen lymphocytes of mice were studied in vitro using single cell gel electrophoresis,and 2,4-dinitrophenylhydrazine measurement was used to detect quantitatively the effects of Taurine on carbonyl compound contents in a reactive system.

采用单细胞凝胶电泳技术检测牛磺酸（50 mmol·L-1）对乙醇醛（GA， 0.1 mmol·L-1）所致小鼠脾淋巴细胞DNA损伤的抑制作用，并用2，4-二硝基苯肼法定量检测牛磺酸对反应体系中乙醇醛含量的影响，同时设乙醇醛阳性对照组和PBS阴性对照组。

Results After 50 mmol·L-1 Taurine was added to the spleen lymphocyte suspension containing 0.1 mmol·L-1 glycolaldehyde, the cellular DNA damage rates were markedly decreased (P.01) and the migration distances of DNA were significantly reduced (P.01) as compared with positive control group, and Taurine reduced the carbonyl compound contents in a reactive system in a dose-dependent manner.

结果：在0.1 mmol·L-1乙醇醛溶液中加入50 mmol·L-1牛磺酸后，小鼠脾淋巴细胞DNA的损伤率较阳性对照组明显降低（P.01），彗星实验的迁移距离也显著缩短（P.01），牛磺酸呈剂量依赖性明显降低反应体系中乙醇醛的含量。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。