淋巴细胞

Methods Subsets of blood T lymphocyte in 93 patients with SARS were detected by flow cytometer.

采用流式细胞仪对 93例临床确诊的SARS患者、5 0例获得性免疫缺陷综合征患者及 6 4例健康体检者外周血T淋巴细胞亚群进行检测。

It plays an important role in activation of T helper cells and cytotoxic T lymphocytes .

在肿瘤免疫中，细胞毒T淋巴细胞的激活、增殖数量和功能是决定肿瘤转归的关键因素。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

Staining and observing shows that the most pathological changes were interstitial pneumonia and hemorrhagic pneumonia, typical nonsuppurative encephalitis, and neurogangliitis et al. The lymphoid tissue was necrosis, and the amount of lymphocytes was reduced. The pathological changes of the positive cells, especially the epithelial cells were swell, denaturization and hyperplasy to the extent.

H.E。染色观察显示，病理组织学变化主要表现为间质性肺炎和出血性肺炎、典型的非化脓性脑炎、神经节炎等；淋巴结、扁桃体和脾脏等组织内淋巴细胞坏死、数目减少；有阳性反应的细胞，特别是阳性上皮细胞呈现不同程度的肿胀、变性、增生等病理变化。

We examined the properties of DC associated with immune suppression in NOD mice by insulin injection subcutaneously and ability to suppress diabetes transfer by diabetogenic effect cells in secondary NOD severe combined immunodeficiency recipients.

NOD小鼠皮下注射牛胰岛素诱导免疫抑制，以血糖检测、胰腺组织学分析和胰岛beta细胞凋亡分析观察自身抗原的免疫保护作用，分离骨髓树突状细胞细胞，流式细胞术分析细胞表面抗原，混合淋巴淋巴细胞反应测定细胞功能。

Myocardial Infarction ; Acute ; T Lymphocytes ; HLA DR Antigens ; Flow Cytometry ; Phytohemagglutinins ; Phorbol 12; 13 Dibutyrate ; Ionomycin ; CD4 CD8 Ratio

心肌梗死。急性； T淋巴细胞；人白细胞－DR抗原；流式细胞术；植物凝集素；沸波醇酯； Ionomycin ； CD4－CD8比值

In this work,atomic force microscopy,near-field scanning optical microscopy,laser scanning confocal microscopy and quantum dot labeling were used to investigate the biophysical properties(the parameters including morphology,membrane nanostructures,membrane pore, friction distribution,adhesion properties,specific interaction of antigen-antibody,distribution mode of membrane receptors and so on) of T lymphocytes stimulated by phorbol dibutyrate plus ionomycin at nanoscale.

本论文基于AFM和NSOM，结合量子点标记及激光共聚焦等技术，以人外周血T淋巴细胞为研究对象，在纳米尺度研究了活化前后以及不同活化阶段T细胞生物物理特性的变化，即细胞形态结构、膜表面纳米结构、膜孔变化、膜表面摩擦力分布、膜表面粘附特性、抗原-抗体间特异性相互作用力、膜表面受体分子分布模式等。

AIM: To investigate the effect of PD98059, a specific repressor of extracellular signal regulated kinase1/2 (ERK1/2), on the proliferation of the murine lymphocytes. METHODS: Lymphocytes were isolated from lymphoid nodes of BALB/c mouse. After stained with carboxy fluorescein diacetate, succinimidyl ester, the cells were stimulated with polyclonal activators, concanavalin and phorbol 12,13 dibutyrate plus ionomycin.

分离小鼠淋巴结细胞，藉羧基荧光素乙酰乙酸染色，用多克隆刺激剂刀豆A蛋白或者佛波醇酯加离子霉素刺激，以流式细胞术分析PD98059对淋巴细胞增殖的影响；利用碘化丙锭染色分析PD98059对细胞周期的影响。

The tissue section of transbronchial lung biopsy specimens showed diffusedly infiltrated small lymphocytes and a lymphoepithelial lesion. CD20 was positive and CD3, CD5, CD10, CD21, CD23, bc12, bc16 were negative in immunohistochemical stain.

支气管镜下可见左肺下叶、右肺中叶支气管狭窄，用支气管镜进行透壁肺活检，病理为小淋巴细胞弥漫浸润，可见淋巴上皮病变；免疫组化CD20阳性，CD3，CD5，CD10，CD21，CD23，Bc12和Bc16阴性。

Methods:The randomly group sampling,parallet comparison and dilamination methods are used in this study.166 cases (10 falling off) was observed on giving priority to diferent treatment of yiqi yangyin,wenbu spleen and kindey,qingre jiedu and evaluate the change of different quantity arrangement of CD4~+T lymphocyte cell of different treatment of TCM on HIV asymptomatic carriers.

采用随机整群抽样、平行对照、分层研究的方法，分别以益气养阴法、温补脾肾法和清热解毒法为主对166例(脱落10例)感染者进行干预，观察中医不同治法对感染者不同层次CD4~+T淋巴细胞的变化。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。