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淋巴细胞

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The results were that PNWⅠ had a significant effect at the concentration of 200μg/mL and PNM had no significant effect on the splenocyte proliferation.

初步考察了PNW和PNWⅠ对小鼠体外淋巴细胞增值的影响,结果显示PNWⅠ在200μg/mL时对淋巴细胞有显著的促进增殖作用,PNW对淋巴细胞的增殖作用不显著。

Objective To establish a method for isolating and culturing the tonsillar lymphocytes,analyze the distribution of lymphocyte phenotypes so as to get some experimental data for peripheral lymphocyte research.

目的 建立人扁桃体淋巴细胞的分离培养方法,研究淋巴细胞的表型分布,为外周淋巴细胞的研究提供实验数据。

Methods The human lymphocytes were isolated from the tonsillar tissues of the patients having a clear clinical diagnosis of tonsil sleep apnea.

目的 建立人扁桃体淋巴细胞的分离培养方法,研究淋巴细胞的表型分布,为外周淋巴细胞的研究提供实验数据。

The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid.

取小鼠外周血非贴壁的淋巴细胞,与靶细胞混合淋巴细胞培养,进行淋巴细胞增殖和反应性T细胞功能检测,并检测细胞培养液中IL-2、TNF-α和IFN-γ水平。

Methods Intrathymic injection of 2.5×107 Wistar spleen cells in neonatal SD rats was performed followed by mixing lymphocyte culture with Wistar rat spleen cells (20Gy) at 4-6 weeks of age. The levels of IL-4 and IFN-γ were measured by ELISA on the third day.

将2.5×107个Wistar大鼠脾淋巴细胞注入新生SD大鼠胸腺内,4~6周龄时取其脾淋巴细胞与Wistar大鼠脾淋巴细胞(20Gy X射线照射)进行混合培养,于72h测定IL-4和γ干扰素的含量。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberines function may be downregulating the expression of ICAM-1 on the HUVEC .

结果:小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附,其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达;小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附,对淋巴细胞表面粘附分子CD18的表达无影响。

RESULTS The results indicated that PCF could significantly increase the viability of thymocytes and enhance the activities of SOD, GSH-Px, CAT in cells. PCF decreased the amounts of ROS and the rate of apoptosis in cells. PCF inhibited the morpholog alteration of the thymocytes irradiated by UVB and maintained the ultrastructure of cells in normal. PCF attenuated UVB caused DNA fragmentation in thymocytes. While combined ERKs inhibitor yielded typical DNA fragmentation, co-treatment of thymocytes with PCF and JNKs and p38 kinase inhibitors completely blocked UVB-induced DNA fragmentation.

结果 PCF能显著提高UVB辐射后小鼠胸腺淋巴细胞的活性;提高胞浆中SOD、GSH-px、CAT的活性,降低ROS含量及细胞凋亡率;抑制UVB辐射所致的胸腺淋巴细胞凋亡的形态学改变,维持细胞的正常超微结构;抑制UVB所致的胸腺淋巴细胞DNA片段的出现;预先应用ERK抑制剂使DNA片段增加,给予JNK和P38抑制剂后协同PCF使DNA片段带减少。

Results The pharmacodynamics studies showed that GLGC could improve PSP excretory of mice airway; compared with model group, the death rate, lung exponent, and blood agglutination of GLGC group reduced obviously, the average life time and the weight of mice improved obviously.

淋巴细胞增殖和NK活性研究结果显示:模型组小鼠脾T、B淋巴细胞增殖能力和NK细胞活性较假手术组均明显降低;瓜蒌甘草颗粒治疗组脾T、B淋巴细胞增殖能力和NK细胞活性较模型组有明显恢复。

To investigate the expressions of Fas and FasL on tumor cells and TILs in patients with so as to understand the potential role of Fas system in immune escape and counteratteck involved in HCC,the expression levels of Fas and FasL were detected on tumor cells,noncancorous liver cells,TILs and PBLs in patients with HCC by means of indirect fluorescent immunoassay flow cytometry.

为研究肝细胞癌患者肿瘤细胞、肿瘤浸润淋巴细胞Fas抗原与FasL配体的表达水平,探讨Fas系统在肝细胞癌免疫逃逸及免疫反击中的作用,采用间接免疫荧光流式细胞术检测20例肝细胞癌患者肿瘤细胞、肿瘤浸润淋巴细胞和外周血淋巴细胞Fas与FasL的表达。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberine's function may be downregulating the expression of ICAM-1 on the HUVEC .

结果:小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附,其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达;小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附,对淋巴细胞表面粘附分子CD18的表达无影响。

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