淋巴细胞

Objective To search an accurate way to determine human leucocyte antigen-B27 of peripheral blood cells.

目的 获得外周血淋巴细胞人白细胞抗原-B27检测准确可靠的结果。

The elder age,underlying diseases,continued high fever, thrombocytopenia,leucocytosis and bilateral lung involvement were the risk factors.

伴发糖尿病与预后转归显著相关，外周血淋巴细胞数下降、血小板减少、血清LDH和CPK升高与预后均有相关性。

The prognosis is poor in patients complicated with diabetes,leucocytosis,lymphocytopenia,thrombocytopenia and elevated serum LDH and CPK.

伴发糖尿病，外周血白细胞增高、淋巴细胞下降、血小板减少，血清LDH和CPK升高的患者预后转归不良。

The neutrophilic leukocytosis is consistent with the presence of CMV infection, although the absence of atypical lymphocytes is less typical.

中性粒细胞增多符合CMV感染，虽然患者无非典型的淋巴细胞。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

According to the design, sample blood and dye; then observe lymphoblast and transitional cell.

将小鼠随机分组后分别应用桑白皮挥发油等试剂，按设计取血染色，并观察淋巴母细胞的淋巴细胞和过渡型细胞。

MTT colorimetry was used to detect the influence of Rg3 on lymphocyte transformation function and NK cytotoxicity. The activity of IL-2 was detected by lymphoblast proliferation assay and IFN-γ by cytopathic effect inhibition.

MTT法检测Rg3对小鼠淋巴细胞转化功能和NK细胞毒活性的影响，鼠脾T淋巴母细胞增殖分析法测定上清中IL-2活性，细胞病变抑制法测定IFN-γ活性。

Methods: The residual lymphoblast percentage of bone marrow was evaluated on day 14 during a four-drug induction in 21 children with ALL. Telomerase activity of bone marrow cell was determined by telomere repeat amplification protocol -silver stain technique at differential clinical stages.

以多药联合化疗方案进行诱导缓解和缓解后治疗，以诱导治疗第14天的骨髓原始淋巴细胞比例作为治疗反应敏感性指标，以端粒重复序列扩增-银染色技术检测端粒酶活性作为微小残留病白血病指标。

In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT－PCR protocol and analysed by polyacrylamide gel electrophoresis.

为检测用EB病毒转化建立的永生性人B淋巴母细胞株中N5，N10－亚甲基四氢叶酸还原酶基因的表达及其cDNA序列，用EB病毒转化人外周血B淋巴细胞，建立永生性细胞株后，从培养细胞中提取总RNA，用RT-PCR法扩增MTHFR基因cDNA的不同片段，进行PAGE电泳分析和cDNA序列测定。

They had higher SCE rate and micro nuclei rate, with 14.12times per cell, and 13.28 times per cell, respectively for SCE rate and 25.2% and 21.2%, respectively for micro nuclei rate, After treated wito mitomytin, they had even higher SCE (38.3、37.2)and micro-nuclei rate(10.7%,10.2%) than normal β－lymphoblast cell line.

现常用细胞EB病毒体外感染外周血淋巴细胞建立淋巴母细胞株，具永生性和不致瘤性，保持原有细胞的生物学特性，为实验室研究提供可信的材料[2]。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。