淋巴细胞

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

The BLSAg appeared in the spleen,the tonsillia cecalia and thymus,the bursa Fabricius,the red marrow and Harderian's gland at the lst week,7th week and 19th week post inoculation.

BLSAg阳性淋巴细胞早期主要分布在胸腺和外周免疫器官的T 淋巴细胞区，第 7周在胸腺、法氏囊和外周免疫器官的T、B淋巴细胞区。

Antibodies induced by allo-TCV may be involved in the mechanisms of T cell vaccination, which include anti -idiotype antidoby specific to allo-T cell vsccine.

淋巴细胞特异的抗体产生，它们均以不同的方式作用于T淋巴细胞。干扰了淋巴细胞的活化，引起免疫小鼠同种移植物的延长。

Results: In acute experiment, there was no significant difference of body weight or organ indexes between the normal control and arsenic poisoning groups ; while the CH50 level, lymphocyte accruing rate, phagocytosis rate and macrophagocyte index in arsenic poisoning group were significantly lower (P.05 or P.01) than those in normal group. There was no significant difference of body weight or organ index between arsenic poisoning group and EXD (10 g) treated arsenic poisoning group, while CH50, lymphocyte accruing rate, phagocytosis rate, and mavrophocyte index were significantly higher in EXD (10 g) treated arsenic poisoning group than those in arsenic poisoning group (P.05 or P.01). In EXD (5 g) treated arsenic poisoning group, results of all the tested indexes were the same as those of arsenic poisoning group.

结果：与正常对照组相比，砷中毒组小鼠体重、脏器指数无明显改变；CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数明显降低，差异有显著性（P.05或P.01；与砷中毒组相比，竹荪治疗低剂量组小鼠体重、脏器指数、CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数无明显改变化；竹荪治疗高剂量组除小鼠体重、脏器指数无明显改变外，CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数明显升高，差异有显著性（P.05或P.01）。

In addition, the influences of BIS on the activities of lymphocytes in vivo and in vitro were studied, including the blast transformation of lymphocytes, the number of acid α-naphthyl acetate esterase positive lymphocyes, the cytostatic effect of hepatic LGL against hepatoma cells, in older to provided the most adequate scientific foundation for the best application of hepatoma immunotherapy.

此外还对上述不同免疫制剂在体应用对正常大鼠血淋巴细胞转化率及酸性非特异性酯酶阳性淋巴细胞数量的影响；不同免疫制剂对分离培养的肝大颗粒淋巴细胞抑肝癌细胞增殖的直接影响的研究，为肝癌免疫治疗的更佳选择提供更充分的科学依据。

On the basis of the percentage of GPI-anchored PLAP conversion, the leucocyte GPI-PLD activities of the 96 leukemia patients were measured. Compared with the 96 healthy controls, the leukocyte GPI-PLD activites of ANLL and CLL patients were significantly increased; the activities of ANLL and CLL patients were significantly reduced.

检测96例白血病患者外周血白细胞GPI-PLD活性时发现，急性非淋巴细胞性白血病和慢性淋巴细胞性白血病患者酶活性较正常人显著性增高，急性淋巴细胞性白血病和慢性粒细胞性白血病患者酶活性较正常人显著性降低。

Affect on the liver pathological state after XIEZHIPING treated: the golden hamster hepatic tissue morphous structure of normal control was normal, which had regular hepatic lobules and no lipid droplet;that of model control manifested diffuse heavy adipose degeneration and heavy ballooning degeneration with inflammatory cell infiltrate which mainly composed of lympholeukocyte;that of high dose group showed midrange ballooning degeneration with chronic inflammatory cell infiltrate which mainly composed of lympholeukocyte and plasmocyte;that of media dose group indicated slight focus ballooning degeneration with a small quantity normal hepatic cells in some locals;that of low dose group appeared midrange adipose degeneration with a few lympholeukocyte cell infiltrate in the parts of central veins and port area.

泻脂平治疗后对肝脏病理状况的影响：空白对照组金黄地鼠的肝组织形态结构正常，肝小叶规则，无脂滴发现；模型对照组肝脏弥漫性重度脂肪变性，发生重度气球样变，伴见以淋巴细胞为主的慢性炎性细胞浸润；泻脂平高剂量组肝细胞呈现中度气球样变，伴见以淋巴细胞、浆细胞为主的慢性炎性细胞浸润；中剂量组肝细胞轻度灶性气球样变，局部可见少量正常肝细胞；低剂量组肝细胞呈中度脂肪变性，可见中央静脉及汇管区些许淋巴细胞浸润。

Affect on the liver pathological state afterXIEZHIPING treated: the golden hamster hepatic tissue morphous structureof normal control was normal, which had regular hepatic lobules and nolipid droplet: that of model control manifested diffuse heavy adiposedegeneration and heavy ballooning degeneration with inflammatory cell infiltrate which mainly composed of lympholeukocyte; that of high dosegroup showed midrange ballooning degeneration with chronic inflammatorycell infiltrate which mainly composed of lympholeukocyte and plasmocyte;that of media dose group indicated slight focus ballooning degenerationwith a small quantity normal hepatic cells in some locals; that of lowdose group appeared midrange adipose degeneration with a fewlympholeukocyte cell infiltrate in the parts of central veins and portarea.

泻脂平治疗后对肝脏病理状况的影响：空白对照组金黄地鼠的肝组织形态结构正常，肝小叶规则，无脂滴发现；模型对照组肝脏弥漫性重度脂肪变性，发生重度气球样变，伴见以淋巴细胞为主的慢性炎性细胞浸润；泻脂平高剂量组肝细胞呈现中度气球样变，伴见以淋巴细胞、浆细胞为主的慢性炎性细胞浸润；中剂量组肝细胞轻度灶性气球样变，局部可见少量正常肝细胞；低剂量组肝细胞呈中度脂肪变性，可见中央静脉及汇管区些许淋巴细胞浸润。

The slice of pathology showed: the control group of infected the small intestines mucosa grew thiner,mucosa grew swelling and congesting,many lymphocytes infiltrated,villus failed off;The small intestines mucosa didnt swell and congest in the group of the leaf of Psidium guajava,little lymphocytes infiltrated under the mucosa and the villus were well; The small intestines mucosa grew swelling and congesting in the control group of treatment,many lymphocytes infiltrated under the villus.

病理切片展示：感染对照组的小肠粘膜变薄，粘膜肿胀、充血，大量淋巴细胞浸润，绒毛脱落；番石榴叶组的小肠粘膜无明显肿胀、充血，粘膜下少负淋巴细胞浸润，绒毛清楚；治疗对照组小肠粘膜肿胀、充血，粘膜－－F人量淋巴细胞浸润。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。