淋巴细胞

The effect of modification was detected by microlymphocytotoxicity test, and the optimal method was conclued by factorial experiment.

用微量淋巴细胞毒试验检测修饰效果，并按析因分析设计方法对实验条件进行优化。

Six healthy no fascioliasis goats confirmed by fecal examination and Dot-ELISA were chosen and randomly divided into Ⅰ,Ⅱ,Ⅲ groups. Each goat of Ⅰ,Ⅱ groups was orally dosed once 200 and 500 Fasciola hepatica metacercariae and during 11 weeks after infection used for the experiment study. Blood sample was collected by jugular vein weekly, to determine IL-2 and TNF-α levels in serum and IL-2 in supernatant with proliferation of PBL of goats infected with Fasciola hepatica.

试验选择6只健康白山羊（经粪便学检查和Dot-ELISA检测，确认无肝片吸虫感染）随机分成Ⅰ、Ⅱ、Ⅲ组，其中Ⅰ、Ⅱ组山羊每只分别1次口服接种200、500个肝片吸虫囊蚴，Ⅲ组作为不感染对照组，每周颈静脉采血1次，连续11周，检测肝片吸虫感染后山羊血清中IL-2、TNF-α水平的变化和外周血淋巴细胞分泌IL-2的能力。

Results: Among 38 transgenic mice surviving beyond one year.12(9 myc and 3 myc＋ ras)mice developed multiple neoplasms.Most tumors were observed as lymphoblastoma and fibrosarcoma in histological types.

结果：在存活一年以上的38只转基因小鼠中有12只(9myc和3myc＋ras)发生多种肿瘤，其组织类型以成淋巴细胞瘤和纤维肉瘤居多。

Interstitial fibrosis and focal lymphocytic infiltration occured in 30% and 20% of patients.

间质纤维化和灶状淋巴细胞浸润分别见于30％和20％的患者。

Methods DNA clone of AD8 and NL43 was respectively transfected to skin by Gene Gun, The expression of virus in the epidermal cells was observed by flow-cytometry and fluoroscope.

以基因枪转染AD8及NL4 3分子克隆至皮肤，流式细胞仪和荧光显微镜观察病毒在表皮细胞中的表达，磁分离术分离CD80 +和CD80 -的游离细胞，进而以PCR和逆转录酶检测游走细胞中HIV对CD+ 4 T淋巴细胞的感染和表达。

Methods Level of delayed-type hypersensitivity, IL-2 of immunized BALB/c mice were determined by footpad swelling,MTT and transfered protective rate of T cells purified by neloy wool were studied with 500LD50 of the bacteria.

方法迟发型变态反应、IL-2分别采用足垫肿胀、MTT法；利用尼龙毛柱纯化免疫的BALB／c小鼠的T淋巴细胞通过尾静脉转移到非免疫小鼠，再用500LD50的STM攻击。

Objective To investigate the expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and intranuclear forkhead/winged helix transcription factor p3 (Foxp3) of splenic regulatory T cells in mice, and compare the effects of different stimuli on the immunological activity of Tregs.

目的 研究体外实验中不同细胞刺激剂对小鼠调节性T细胞表面T淋巴细胞毒性相关抗原-4(CTLA-4)及叉头翼状螺旋转录因子(Foxp3)表达的影响，并对不同细胞刺激剂在Treg抑制活性发挥中的作用进行比较。

Bronchial asthma is the most common chronic diseases in children.Asthma can not be fully explained by imbalance of Thl/Th2.With the research progress of CD4+ CD25+ Treg cell,it has been found that CD4+ CD25+ Treg cell related factors such as forkhead/winged helix transcription factor,heine oxygenase-1,transforming growth factor-β,cytotoxic T lymphocyte-associated antigen-4 are closely linked to asthmatic mechanisms.

支气管哮喘是一种儿童时期最常见的慢性疾病，单纯的Thl/Th2应答失衡并不能完全解释支气管哮喘的发生，伴随着CD4+CD25+调节性T细胞的研究进展，人们发现与CD4+CD25+Treg细胞相关的因素如叉头/翼状螺旋转录因子、血红蛋白加氧酶-1、转化生长因子β、细胞毒性T淋巴细胞相关抗原-4与支气管哮喘均有密切的联系。

Microscopically, edema、 small hemorrhagic spot on superficial mucosa, superficial epithelium was necrotic and scaled, foveola epithelial cells proliferated; lymphocytic and plasma cells infiltrated into the mesenchyme of lamina propria, sometimes neutrophil and eosinophilic granulocyte done.

镜下，粘膜浅层有水肿、出血点，表浅上皮坏死脱落，小凹上皮细胞增生；固有层间质中可见淋巴细胞和浆细胞浸润，有时可见少数中性粒细胞和嗜酸性粒细胞浸润。

Objective:To make sure if fusing with activated B lymphocytes could improve the immunogenicity of tumor cells.

摘 要 目的：了解肿瘤细胞与活化B淋巴细胞的融合能否提高前者的免疫原性。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。