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淋巴细胞

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Objective:To study the hypoxanthine phosphoribosyl transferase gene mutation of rat peripheral blood lymphocytes and spleen lymphocytes induced by intermal radiation and of human peripheral blood lymphocytes induced by Y -ray.

目的:本实验主要是用克隆法研究放射性核素内照射诱发Wistar大鼠外周血淋巴细胞和脾淋巴细胞次黄嘌呤鸟嘌呤磷酸核糖转移酶基因突变及γ射线外照射诱发健康成年人外周血淋巴细胞HPRT基因突变情况。

Apoptosis of lymphocytes in bone marrow, spleen, thymus and bursa was detected by light microscope, electron microscope and terminal- deoxynucleotidyl transferase UIP nick end labeling. The results showed ALV-J could induce incoordinate apoptosis of lymphocytes in thymus, bursa and spleen, which was mainly found in early time. And the apoptosis was much more obvious in thymus than in other immune organs.

以电镜观察和HE染色、原位末端标记染色光镜观察对ALV-J感染和混合感染鸡的骨髓、脾脏、胸腺、法氏囊等免疫器官中淋巴细胞的凋亡进行了形态学研究,首次发现ALV-J可诱导胸腺、法氏囊和脾脏中不同程度的淋巴细胞凋亡,凋亡主要见于早期,ALV-J与REV混合感染时淋巴细胞凋亡尤为显著,以胸腺中最为明显。

We also have observed some organs pathological changes of juvenile Jian Carp, such as pancreas acinous gland and zymogen granules of acinous gland cell disappearing, intercellular substance hyperplasia and inflammatory cells soakage, hepatatrophia, liver cell granular or vacuolar degeneration and necrosis, karyolysis or pyknosis, glycogen granules decreasing, metanephros atrophy, metanephric canaliculus epithelium granular or vacuolar degeneration and necrosis, mitochondrion swelling and mitochondrion cristae disappearing, karyolysis, distal convolutal tubule microvilli desquamating, spleen marrow cell degeneration and necrosis, intercellular substance of spleen hyperplasia, spleen atresia, blood corpuscle disappearing.

后肾土黄色、淡褐色或苍白色,肾小管上皮细胞肿胀、颗粒或水泡变性、坏死,细胞内有大量血细胞流出,线粒体肿胀,嵴结构消失,细胞核溶解,肾间质甲状腺滤泡和拟淋巴细胞增生,远曲小管微绒毛脱落、管道细胞界限不清。心脏肌纤维肿胀、颗粒或空泡变性,严重的肌纤维溶解、变细或断裂,肌纤维间水肿、炎性细胞浸润,部分心肌细胞核浓缩。脾脏暗褐色,脾髓质细胞变性、坏死,拟淋巴细胞明显减少,淋巴细胞岛少见、岛中细胞成份减少,黑素巨噬细胞中心减少、体积缩小,脾脏网状基质水肿,脾窦闭锁,血细胞减少。

Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3H] thymidine incorporation assay. Effects of ginsenodide-Ro on the production of cyrokines interleukin-2 (IL-2), interferon-γ and interleukin-4 (IL-4) from mutine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of TH1 cytokine IFN-γ and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction analysis.

方法[3H] TdR参人法检测人参皂有-Ro对小鼠脾淋巴细胞增殖的影响;酶联免疫吸附法检测人参皂苷-Ro对小鼠脾淋巴细胞产生细胞因子白介素-2、干扰素-γ和白介素-4的影响;逆转录聚合酶链式反应分析法研究人参皂苷-Ro对小鼠脾淋巴细胞引干扰素-Υ、白介素-4mRNA表达的影响。

Objective To detect the expression of CD70 in peripheral T lymphocytes of patients with systemic lupus erythematosus and the effect of azacitidine, an inhibitor of DNA methylation, on it. Methods Blood samples were obtained from 10 patients with active SLE (SLEDAI score ≥5), 10 patients with nonactive SLE (SLEDAI score 5) and 10 normal human controls.

目的 通过研究共刺激分子CD70在SLE患者外周血T淋巴细胞的表达水平,以及DNA甲基化抑制剂氮杂胞苷对正常人外周血T淋巴细胞表达CD70分子的影响,探讨DNA甲基化在SLE患者外周血T淋巴细胞表达CD70分子中的调控作用。

The immune level tested in different time after injection with ginkgolic acids in sensitization mode showed that the expression of CD3〓 and CD4〓T lymphocytes increased, but CD8〓 was restrained, while the B lymphocytes was also increased. The results suggested that sensitivity was approved and T lymphocytes proliferation was induced, which strength cell immunity responsion, at the same time B lymphocytes was differentiated to IgE antibody-producing cells when contacted with ginkgolic acids as allergen.

大鼠经致敏方式接触银杏酸后,在不同时间的细胞免疫水平与体液免疫水平的研究结果表明,大鼠CD3〓及CD4〓T淋巴细胞的表达增加,而CD8〓T淋巴细胞的表达受到明显抑制,同时参与体液免疫的B淋巴细胞百分率呈增高趋势,提示银杏酸可能作为变应原使机体敏感性增强,诱导T细胞分裂增殖,增强细胞免疫应答,同时促进B细胞增殖分化成IgE抗体产生细胞。

Objective:in order to compare the curative effects of using lamirudine and thymosin together with using thymosin only to the paients of chronic hepatitis b.methods:we had chosen 27 medical cases of chronic hepatitis b,which were divided into two groups:15 cases asone group,and 12 cases as the other group.let group a take 100 mglamirudine orally,qd,with half a year as a period of treatment.at the same time givethe patients injections of 100 mg thymosin,qd,for a month.to group b was only takenlamirudine.alt,tbil,the indicators of the virosis of hepatitis b (hbsag,hbeag,hbv-dna)were observed as quatos.results: at the end of the treatment, the rates that groupa and group b made hbsag transform into negative were respectively 26.67% and 16.67%(p>0.05);hbeag:33.33%,8.33%;hbv-dna:53.33%,33.33%(p>0.05).conclusion:combining thymic peptide with 3tc to treat chronic type b hepatitis, thymic peptide will not only promote b-cell to mature and transformation, but also to keep the subpopulation of t-cell in immune system in balance, which can enhance the reaction of matured t-cell to the antigen or other stimulus.

目的:比较拉米夫定(3tc)与胸腺肽联用和单用拉米夫定治疗慢性乙型肝炎的疗效。方法:选择慢性乙型肝炎患者27例,分为联合组15例和拉米夫定组12例;联合组用拉米夫定100 mg,口服,qd,疗程半年,合并用胸腺肽100 mg,静滴,qd,疗程1个月。拉米夫定组单用拉米夫定。以谷丙转氨酶、血清胆红素、乙肝病毒标志物(hbsag,hbeag,hbv-dna)为观察指标,比较不同治疗方案对指标的影响。结果:治疗结束时联合组与拉米夫定组对慢性乙肝患者血清hbsag阴转率分别是26.67%和16.67%(p>0.05);hbeag阴转率分别是33.33%和8.33%(p>0.05);hbv-dna阴转率分别是53.33%和33.33%(p>0.05)。结论:胸腺肽联合3tc治疗慢乙肝,胸腺肽具有促进t淋巴细胞的成熟和转化,并维持免疫系统各t淋巴细胞亚群间的平衡作用,能增强成熟t淋巴细胞对抗原或其他刺激的反应。

The cancers studied here were throat cancer, aute non- lymphoblas He leukem ia and acut lymphoblastic leukemia. The result s indicated that,(1)the levels of Leu, Lys, Ser Asn,Ile,Gly,and Thr were obviously decreased whea lse the levels of orn, Trp evidently increased in cultural liquid of throat can cer cell line indicating that the growth of HepⅡ cell consumed 7 amino acids and releaced 2 amino acids into cultural liquid.(2)the levels of Glu, Gls, Leu, Phe, Tyr and Trp were evidently increased while the levels of Thr , His, Ala decreased in plasma from patient with ANLL. These r esults were consistent with the previous report.(3)the levels of Gly, Trp and Phe in plasma from patients with ANLL evidently de creased and the levels of Ala, His increased after therapy indicating that cance r cells were growing in an anaerobic catabolism state.

这些癌肿包括喉癌HepⅡ细胞,急性非淋巴细胞白血病和急性淋巴细胞白血病,结果表明:(1)喉癌细胞株培养过程中亮氨酸、赖氨酸、丝氨酸、天冬酰胺、异亮氨酸、甘氨酸以及苏氨酸等水平明显降低,而色氨酸水平明显增加,说明喉癌细胞的生长繁殖必须依赖以上7种氨基酸同时释放了色氨酸;(2)急性非淋巴细胞白血病患者血浆中的谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸等水平明显升高,而苏氨酸、组氨酸、丙氨酸等水平明显降低,这些结果与国际报道相一致;(3)经治疗后,ANLL患者血浆中甘氨酸、色氨酸和苯丙氨酸等水平明显降低,而丙氨酸、组氨酸等水平明显升高,表明肿瘤细胞处在无氧代谢。

Results ① The percentage of positive TPOAb and TGAb were 91.4% and 74.4%, respectively in all the AIT patients. 47.6% of the patients had TSH levels within normal range (0.3~5mu/L).② All of the slides had different grades of lymphocytic infiltration. 49.3% had germinal center, 32.8% had Askanazy cells, 26.9% had plasma cells, 22.4% had colloid, and 9% had multinuclear giant cells.③ Lymphocytic infiltration was divided into four degrees. The levels of TSH and TPOAb increased significantly in the extremely heavy lymphocytic infiltration grade than in the others (P.05). There was no relationship between serologic markers and other cytopathologic features.

结果 ①82例患者中TPOAb、TGAb的阳性率分别为91.4%和74.4%,约有半数(47.6%)TSH值位于正常值范围内(0.3~5mu/L);②所有患者细胞病理学均存在淋巴细胞不同程度的浸润,49.3%可见生发中心,32.8%可见嗜酸性变细胞,26.9%可见浆细胞,22.4%可见胶质,9%可见多核巨细胞;③将淋巴细胞浸润程度分级,分别与TSH、TPOAb及TGAb进行相关性分析,发现仅淋巴细胞极重度浸润者其血清TSH、TPOAb水平较其他组显著升高(P.05),其他细胞病理学改变与血清学指标之间未发现显著相关性。

Result:① In NC group,the levels of plasmic and lymphocytic NO were higher in the morning than those in the afternoon.② The plasmic and lymphocytic NO were significantly decreased in CAD patients then those in NC group (P<0.01).③The physiological NO curve disappeard in CAD patients.The concentrations were no obvious changes and maintained lower levels in 24 hours.Conclusion:As similar as other endocrinic hormones,the NO secretion was not equal in 24 hours.

结果:①正常人24 h血浆和淋巴细胞中NO分泌呈上午高、下午低的趋势,晨6时最高,下午6时呈低谷,晚10时起又渐升高至早晨;②CAD心绞痛患者血浆和淋巴细胞中NO含量明显低于正常人,其差异具有统计学意义(P<0.01);③CAD患者失去了正常的NO生理性分泌曲线,血浆和淋巴细胞中24 h均在较低水平,波动范围不大。

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