淋巴细胞

When SD rats were exposed to a noise intensity of 108 dB with a main frequency of 3.6 kHz for a duration of 6 hours,per day,the contents of RLGC for the experimental group was significantly lower than that of the control group.Active units of GTR in rats were significantly higher than those of the control group,while lymphocyte blastogenesis and act...

当SD大鼠暴露在噪声强度108 dB，主频率3.6 kHz，主频对应最大峰值97 dB下持续一定时间时，实验RLGC含量噪声组明显低于对照组，大鼠GPT活性单位噪声组明显高于对照组，大鼠淋巴细胞转化率和活性花环形成率噪声组明显低于对照组，说明舰艇机舱噪声对SD大鼠的生化指标具有明显的影响。

Key words:turbine noise;SD rat;liver glycogen;GPT;lymphocyte blastogenesis;active rosette forming cell

关键词］舰艇机舱噪声；大鼠；肝糖原；谷丙转氨酶；淋巴细胞转化率；活性花环形成率

Results interleukin2activity,interleukin2receptor expression of lymphocyte membrane and lymphocyte blastogenesis in peripheral blood were inhibited at and above30PPM concentration of fluorine ion.

结果 当氟离子浓度达30PPM时，即可抑制白细胞介素2活性、淋巴细胞膜白细胞介素2受体表达及外周血淋巴细胞转化功能。结论提示氟化钠具有免疫毒性。

The nitrogen balance on days 8 was remarkably improved in experimental and normal diet groups compared with control group. There were highter serum glutamine and BCAA concentration, hydroxyproline content and tensile strength of wound, and better lymphocyte blastogenesis in experimental group compared with control and normal diet groups. Experimental and normal diet groups had higher mucosal DNA, RNA and protein content than control group.

实验组动物的血浆谷氨酰胺、BCAA含量，外周血淋巴细胞转化率，伤口皮肤抗张力强度和羟脯氨酸含量都显著高于对照组和正常饲料组(P＜0.05)；实验组和正常饲料组动物第8天氮平衡，小肠粘膜核酸和蛋白质明显高于对照组(P＜0.05)。

Turbine noise ; SD rat ; liver glycogen ; GPT ; lymphocyte blastogenesis ; active rosette forming cell

舰艇机舱噪声；大鼠；肝糖原；谷丙转氨酶；淋巴细胞转化率；活性花环形成率

On days 14, serum proteins and amino acids concentration and peripheral lymphocyte blastogenesis were determined. At the end of the study, the animals were sacrificed, hydroxyproline and tensile strength of wound, and intestinal mucosal DNA, RNA and protein content were examined.

测定饲料摄入量、体重、氮平衡、血浆蛋白和氨基酸含量、外周血淋巴细胞转化率、伤口埋植海绵中羟脯氨酸含量、皮肤抗张力强度和小肠粘膜蛋白质及核酸。

Methods Guinea pigs were, to begin with, given MBP through the nasal route to induce the development of peripheral immune tolerance, and then immunized with specific antigen MBP plus complete Freund's adjuvant. The clinical symptoms of guinea pigs were investigated, and the erythrocyte rosette test, and lymphocyte blastogenesis test, the MBP-specific skin delayed-type hypersensitivity response and the levels of interlukin-4 (IL-4) and interferon-γ were also detected, and the pathological changes were examined as well.

先使豚鼠经鼻粘膜摄入MBP，诱导建立外周免疫耐受，然后用MBP加完全弗氏佐剂免疫豚鼠，观察动物的临床症状、E花环形成试验、淋巴细胞转化试验、迟发型超敏反应、细胞因子水平及病理改变。

We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我们采用了一种利用单细胞标本通过二重巢式PCR并结合ARMS技术确定中国人群中广泛的β-地中海贫血突变谱的基因型的策略，通过对含有β-珠蛋白基因CD41-42突变的携带者5个该基因常见突变位点基因型的确定，以及正常人的单个淋巴细胞、单卵裂球的β-珠蛋白基因该5个位点基因型的确定，对这种技术条件的优化、准确性和可靠性进行了研究。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域，用限制酶 Bfm 消化 PCR产物，10 %聚丙烯酰胺凝胶电泳检测。

Methods Multiplex nested PCR was used to amplify the sex linked amelogenin and IL-3 genes from human single lymphocytes and single blastomere.

方法取成人外周血单个淋巴细胞和单卵裂球，采用多重巢式多聚合酶链技术同时扩增性别相关基因Amelogenin基因和IL-3基因。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。