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淋巴细胞

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Obstructive jaundice was induced in the mice in the experimental group by common bile duct ligation. The level of the common bile duct diameter, WBC, LYM MID, LYM%, MID% and ALT, AST, TBIL, DBIL, IBIL, ALP and CHOL were measured 12 h and 1, 2, 3, 4, 5, and 7 days after the ligation.

于实验的第12 h,1、2、3、4、5、7 d动态观察胆总管直径及白细胞总数、淋巴细胞总数、中间细胞总数及淋巴细胞总数百分数和中间细胞总数百分数,测血清总胆固醇、丙氨酸氨基转氨酶、天冬氨酸氨基转氨酶、碱性磷酸酶、总胆红素、直接胆红素及间接胆红素值。

Results and ConclusionThe study of time-efficacy relation expresses that after infusing the solution of Phyuanthus emblica Phyllanthus emblica, medicated serum in different time activates the spleen lymphocyte of small mice differently after the 30min but before 180min. During this period, on 2h the activation of the medicated serum is the most obvious. The quantity-efficacy relation research expresses that megadose promotes the propagate of the spleen lymphocyte of small mouse and activates the macrophage in the abdominal cavity more obviously. On the other hand, the small dosage influents the survival rate of the mouse S180 ascites tumor cell more obviously.

结果与结论时效关系研究表明,用余甘子冻干粉溶液灌胃后30~180 min内不同时相含药血清对小鼠脾淋巴细胞具有活化作用,并在给药2 h时实验血清活化作用明显;量效关系研究表明,余甘子大剂量组实验血清对促进小鼠脾淋巴细胞增殖,促进小鼠腹腔巨噬细胞细胞活化作用以及小剂量组实验血清对小鼠S180腹水肿瘤细胞存活率的影响均具显著差异性。

Much more prolongation ofsurvival term and improvement of survival rate,as well as appearance of a great dealof lymphocytes around pulmonary metastatic nodes and less pulmonary metastaseswere also observed in murines treated with activated、tumor antigen-pulsedmacrophages contrast to those treated with IL-2 gene-transfected macrophageswithout prior pulse of tumor antigen in vitro.

肿瘤抗原冲击的IL-2基因转染巨噬细胞治疗组小鼠期存活明显延长,肺转移结节明显减少,局部病理显示,肺肿瘤周围出现大量淋巴细胞浸润,我们推测这可能是因为抗原冲击的活化巨噬细胞除了增强对肿瘤细胞直接的杀伤效应外,还对其进行处理加工,将免疫信息传递给周围的淋巴细胞,从而引起其增殖活化,并浸润肿瘤组织中。

Using Flow cytometer,TriTest reagent and Trucount tube(TriTEST/TruCOUNT)to assess absolute counts of CD 3,CD 4 and CD 8T lymphocyte subsets and CD 4/CD 8 ratio and observe T lymphocyte subsets distribution in sex,age,ethnics in the 110 healthy adults(Age∶16-56)in Guangxi.

用流式细胞仪(FACSCalibur,FCS)、三色免疫荧光试剂和绝对计数管(TnTEST/Tru COUNT),检测 110名广西健康成年人(16~ 5 6岁)静脉全血CD3 、CD4、CD8T淋巴细胞绝对数值和CD4/CD8比值,并观察T淋巴细胞亚群分类计数在性别、年龄和民族上的差别。

However, the study about the relationship between the number of TILs and non-small-cell lung cancer is very few, especially between CD4~+、 CD8~+T lymphocytes and NSCLC.

研究发现某些肿瘤(如,食道癌、胰腺癌、结肠直肠癌)浸润淋巴细胞(tumor infiltrating lymphocyte,TIL)的数量和T细胞亚群与肿瘤的预后有明显的关系,但肿瘤浸润淋巴细胞,特别是CD4~+、CD8~+T细胞亚群与非小细胞肺癌(non-small-cell lung cancer,NSCLC)预后的研究很少,说法不一。

Results showed that the expressions of both Fas and FasL on tumor cells were up-regulated compared with noncancerous liver cells from same patient.When compared with the PBLs from same patient,the expression of Fas on TILs was highly up-regulated whereas the FasL down-regulated.

结果显示,肝细胞癌患者肿瘤细胞Fas与FasL表达水平较自身非肿瘤肝细胞上调;肿瘤浸润淋巴细胞与自身外周血淋巴细胞相比,Fas表达上调而FasL表达下调。

Using a model of the muscle pouch of bone graft, the effects of the systemic and local administration of IL-4 plus IL-10 on bone xenograft rejection were studied through the determination of lymphocyte responses from the mixed lymphocyte culture , subsets of lymphocyte, cytokines and histology in 1, 2, 4, and 6 weeks after implantation.

分别于术后1、2、4、6周取材,进行淋巴细胞刺激增殖实验、淋巴细胞亚群分析、细胞因子检测及组织学检查,了解移植免疫特点,并观察经腹腔和局部注射IL-4和IL-10后产生的免疫抑制作用。

The results showed that the starved fish had significant higher white-cell counts than the fed fish,the peak value occurring 20 d after starvation.There were higher multiplication and efficiency of transformation in the lymphocytes in the starved fish than those in the fed fis...

结果表明:饥饿后红鳍东方鲀外周血中白细胞的数量明显高于正常投喂组,且在饥饿20 d时白细胞的数量达到最大值;饥饿后脾脏中淋巴细胞的增殖转化能力与转化率比正常投喂组高,饥饿10 d时达到最大值,随着饥饿时间的延长,淋巴细胞的转化率逐渐降低;饥饿后脾脏中巨噬细胞的吞噬活性与吞噬率均高于正常投喂组,且在饥饿20 d时达到最大值。

This experiment unified flow cytometry, the monoclonal antibody technology, the cell fluorescence chemistry technology and unilized the union principle of antigen and antibody to examine T lymphocyte subgroup, the B lymphocyte change in circumference blood of the mice through the flow cytometry; At the same time, the agarose gelatin electrophoresis examined bovine colostrum effect on programmed cell death of thymus.

本实验正是将流式细胞术、单克隆抗体技术、细胞荧光化学技术相结合,并利用抗原抗体结合原理,采用流式细胞仪检测了小鼠外周血的T淋巴细胞亚群和B淋巴细胞;同时采用琼脂糖凝胶电泳技术检测了牛初乳对小鼠胸腺细胞凋亡的影响。

This paper dealed with the immunnodulation tests of white wax scale in specific and unspecific immunity. The result showed that the IgM in high dose group was clearly higher than that of the control in serolysin test. Lymphocyte transformation and propagation in mice were notable in high dose group.

对白蜡虫的特异性和非特异性免疫调节功能进行了试验研究,结果表明:特异性免疫的血清溶血素试验中,白蜡虫高剂量组小鼠的溶血素生成量明显高于对照,淋巴细胞转化增殖试验中,白蜡虫高剂量组小鼠的淋巴细胞转化增殖明显。

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