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淋巴细胞

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Results: As compared with those of the irradiation control groups, the rhCuZnSOD showed significant beneficial effects on immunocytes, these included enhancing the proliferative response of T lymphocyte to concanavalin A and the activity of natural killer cells in spleen, increasing the number of whole spleen lymphocyte, leucocyte and lymphocyte counts in peripheral blood.

与照射对照组比较,rhCuZnSOD可减轻电离辐射对小鼠免疫细胞的影响,其作用包括增强脾T淋巴细胞对刀豆蛋白A诱导的增殖反应,提高脾自然杀伤细胞的活性,升高全脾淋巴细胞及外周血白细胞和淋巴细胞的数量。

Significant levels of human immunoglobulin were detected in sera of hu-PBL-SCID mice 4 weeks after engraftment.

在移植后4周,其小鼠血清中存在人免疫球蛋白,其脾淋巴细胞中,所检测的8 种免疫表型人淋巴细胞亚群均存在,表明人淋巴细胞在SCID小鼠体内出现了增殖或重建。

Small intestinal biopsies showed subepithelial collagen deposition with varying degrees of villous atrophy and varying numbers of intraepithelial lymphocytes. Four patients had previous biopsies showing enteropathic changes without collagen deposition. Seven cases were associated with collagenous colitis and 1 also had features of lymphocytic colitis. Three patients also had collagen deposition in gastric biopsies. One case was associated with lymphocytic gastritis. Celiac disease (CD, gluten-sensitive enteropathy) was documented in 4 patients. Five patients made a clinical improvement with combinations of a gluten-free diet and immunosuppressive therapy. Two patients died of complications of malnutrition and 1 of another illness. Clonal T-cell populations were identified in 5 of 6 cases tested. Four of these patients improved clinically after treatment but 1 has died. Collagenous sprue evolved on a background of CD in 4 cases. There was no history of CD in others and these cases may be the result of a biologic insult other than gluten sensitivity.

小肠活检表现为上皮下胶原沉积,绒毛萎缩程度不一,上皮内淋巴细胞浸润数量不等。4例患者以往曾做过活检,表现为其他肠病改变,并没有胶原沉积。7例患者合并胶原性结肠炎,且1例还有淋巴细胞性结肠炎的特征。3例患者胃活检也发现有胶原沉积。1例患者合并淋巴细胞性胃炎。4例患者有乳糜泻(CD,谷蛋白敏感性肠病)病史。5例患者经无麸质饮食和免疫抑制治疗后,临床症状有所改善。2例患者因营养不良合并另一种疾病而死亡。6例患者做了T细胞受体基因重排,其中5例发现有克隆性T细胞群,这5例中有4例治疗后临床症状加重,且1例死亡。4例患者在CD的基础上病发胶原性口炎性腹泻;其余患者无CD病史,他们的发病也许是生物源性损害,而非谷蛋白敏感。

Methods The DNA injury was determined with single cell gel electrophoresis, the contents of NO and NOS were measured by NO and NOS kits respectively and apoptosis of lymphocytes was determined by flow cytometry and gelose electrophoresis.

建立硫芥中毒淋巴细胞模型,分别用单细胞凝胶电泳法检测淋巴细胞DNA损伤;用镀铜镉还原法和一氧化氮合成酶试剂盒检测中毒后淋巴细胞培养上清液一氧化氮和一氧化氮合酶的变化;用流式细胞仪和琼脂糖凝胶电泳法检测细胞凋亡。

The results show that the T and B lymphopoiesis induced by ConA and LPS respectively were evidently promoted by astrglan and astragalin with determinate magnetization parameters. The best magnetization parameters for T and B lymphopoiesis are different.

结果表明,在一定磁场处理条件下,对ConA诱导的鸡T淋巴细胞增殖和LPS诱导的鸡B淋巴细胞增殖有显著促进作用;T淋巴细胞和B淋巴细胞增殖反应的最优磁化条件不同。

Results The frequency of CD70+CD4+ lymphocytes was 14.55%± 5.49% in normal control group, 85.25%± 14.08% in active SLE group, 77.65%± 18.77% in nonactive SLE group, and 81.54%± 8.71% in methylation-inhibited group. Compared with the normal control group, a significant increase was observed in both the frequency of CD70+CD4+ lymphocytes (all P 0.01) and the expression of CD70 expression (all P 0.05) in other three groups.

结果 正常人外周血CD4+CD70+ T淋巴细胞阳性率为14.55%± 5.49%,活动期、非活动期SLE分别为85.25%± 14.08%和77.65%± 18.77%,甲基化抑制组为81.54%± 8.71%,与正常人对照组相比,活动期、非活动期SLE患者和甲基化抑制组CD70+CD4+ T淋巴细胞阳性率均明显升高(P 0.01),其外周血T淋巴细胞CD70 mRNA表达水平亦明显增加(P 0.05);SLE患者外周血CD70+CD4+ T淋巴细胞阳性率与SLE疾病活动度呈显著正相关(r = 0.72,P 0.05)。

The sry-gene chimerism of bone marrows, spleen and thymus of the receiptor, T-lymphocyte subpopulation of peripheral blood cells, ConA and LPS induced T and B cells proliferation activity tests, Mixed lymphocyte reaction between the donor and the receiptor and the third part, the incidence rate of GVHD and survival analysis were observed in mice after 8Gy TBI with MSCs and haplo-BMT treatment together.

通过观察半相合BMT联合MSCs治疗致死剂量照射小鼠后不同时间骨髓、胸腺和脾脏中供者细胞植入,外周血中的淋巴细胞亚群,ConA和LPS刺激的T、B淋巴细胞增殖,供受者及第三者混合淋巴细胞反应,急、慢性GVHD的发生率和生存分析,提示MSCs促进骨髓干细胞植入,加快T淋巴细胞增殖,促进体液免疫恢复,通过供受者免疫耐受,减少GVHD发生率,提高半相合BMT动物生存率。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子,CD83分子表达明显高于国内报道;肝癌患者DC培养上清中TNF水平高于健康人,提示肝癌患者DC仍具一定的抗原呈递能力,适当调控可使其行使APC功能,以期在肝癌个体化疫苗中发挥作用;DC负载肝癌可溶性抗原后,抗原呈递能力降低,BCG HSP70可促进DC成熟,增加其抗原呈递能力,预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子;肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞,活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主,同时分泌较高水平Th1型细胞因子IL-12和TNF,并抑制4种肝癌细胞生长;冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤,提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用;用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽,在体外诱导肝癌特异性淋巴细胞,活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF,并具较强杀伤活性,此CTL同时具备NK细胞特征;DC对肿瘤细胞的抑制作用可能是通过吞噬实现的,Fas-L在DC抑制中也起一定作用;DC对人肝癌裸鼠皮下移植瘤的抑制率为97%;在肝癌DC疫苗的作用中,至少联合3种以上机制,即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示,重组疫苗接种1周后,免疫鸡产生抗IBV的抗体;而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组;攻毒后,异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势,并且该组低水平CD4+的状态一直持续到试验结束,而其他组CD4+ T淋巴细胞均迅速上升,峰值达到14.5 %;同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达,而其他组攻毒后均无明显变化;保护率结果显示,同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %,与其他各组相比均有显著差异;另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻,其排毒时间和排毒量也均有所减少;强毒攻毒后所有试验组体重无显著差异。

There were both T and B lymphocytes infiltration in the tumor microenvironment of the three kinds of tumors, but most of TILs were Tlymphocytes in which CD8~+T cells were the main lymphocytes. Therewere scarce NK cells infiltration in the three kinds of tumors. 2. The distribution of TILs in the three kinds of tumors was different.

三种肿瘤组织中TILs的分布不同:伴较多淋巴细胞浸润的IMPC中,TILs以间质浸润淋巴细胞为主;而MC和AMC中,TILs以癌巢间浸润淋巴细胞(intraepithelial组)为主,更有利于淋巴细胞充分发挥其抗肿瘤效应。

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