淋巴细胞

Objective To investigate the effects of aloe-emodin on PHA-induced proliferation and intracellular Ca2 mobilization of T lymphocytes.Methods The proliferation of T cell was tested by MTT assay.

目的研究芦荟大黄素对植物血凝素引起的大鼠T淋巴细胞增殖和细胞内Ca2 浓度([Ca2 ]i)变化的影响，探讨芦荟大黄素对T淋巴细胞增殖的作用和机制。

Aloe-emodin blocks PHA-induced proliferation of T lymphocytes and the mechanism for this may be due to its inhibition on PHA-induced [Ca_(2 )]_imobilization.

结论芦荟大黄素可抑制T淋巴细胞增殖，其作用机制可能与抑制淋巴细胞内[Ca2 ]i升高相关。

Methods:Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain, cell ELISA, immunocytochemistry techniques to investigate the effect of berberine on lymphocyte-endothelium adhesion and the molecular mechanism.

以小檗碱处理人淋巴细胞或人脐静脉内皮细胞后，用蛋白染料染色法研究对淋巴细胞与内皮细胞粘附的作用，用细胞ELISA、免疫细胞化学染色法研究对细胞表面粘附分子表达的影响。

The results display that the thymus-index, spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 in the experiment groups were significantly higher than those of the control group (P.05); the spleen's lymphocytes translation stimulate indexes in the experiment groups were higher than that of the control group at a significance level(P.01). The peritoneal macrophages can enhance the ability of swallowing. The ability of licking up neutral red was depended upon the dosage of drug.

与对照组相比，试验组的胸腺指数、脾脏指数、免疫球蛋白和补体C3、C4都升高且显著水平为差异显著(P.05)；脾脏淋巴细胞转移刺激指数均极显著地高于对照组(P.01)， 75μg/mL组的脾脏淋巴细胞转移刺激指数达最高；腹腔巨噬细胞具有一定的吞噬能力，吞噬中性红能力与剂量成依赖关系。

The test groups were drenched orally with different dosages of compound Radix scutellariae granules. After being drenched orally for ten days, their thymus-index and spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 of serum were defermined, and experiments in vivo were done. The mice-spleen's lymphocytes which were cultured in vivo were added with different concentrations of compound Radix scutellariae granules. The lymphocytes translation stimulate indexes were measured. The ability of licking up neutral red of the peritoneal macrophages cultured in vivo was measured in the groups.

应用复方黄芩颗粒给小鼠灌胃10d后，分别测定对照组与实验组胸腺指数、脾脏指数和血清中的免疫球蛋白IgA、IgG、IgM和补体C3、C4；并且进行体外实验，用不同浓度的复方黄芩颗粒剂对小鼠脾脏淋巴细胞进行体外培养，测定淋巴细胞转移刺激指数；在体外培养测定细胞吞噬中性红的能力。

The proliferation rate of spleen lymphocytes cocultured with MBT2B71 cells was determined with MTT assay.

采用混合淋巴细胞培养MTT法，观察MBT2B71细胞刺激脾淋巴细胞增殖情况。

ObjectiveTo probe into the hyperplasy effect of different curcumine administered approaches for the splenic lymphocyte of mice.

目的探讨姜黄素3种不同给药途径对小鼠脾淋巴细胞增殖的影响。方法通过噻唑蓝比色法比较姜黄素3种给药途径对小鼠脾淋巴细胞增殖的影响。

The rats were killed by cutting theirnecks and physical blood was taken for preparing serum, interleukin 2 (IL-2) was detected by radioimmunoassay ; the spleen was taken for measuring ConA-induced reaction of lympho- cyte proliferation and induced production of IL-2; and the activity of induced production of spleen's IL-2 was determined by the means of cell proliferation of mouse thymus; the skin and trachea were taten for slice with paraffin wax, dyeing with methybenzoamine blue so as to survey mast cells .

将大鼠断头留取躯干血制备血清，用RIA法检测白细胞介素2（IL-2）；取脾脏行ConA诱导的淋巴细胞增殖反应测定和IL-2诱生；并用小鼠胸腺细胞增殖法检测脾IL-2诱生活性；取皮肤和气管做石蜡切片、甲苯胺兰染色检测肥大细胞，结果显示：应激组较对照组脾淋巴细胞增殖降低，cpm分别为9466±3.17和24019±3879（P.001）；MC明显减少，分别为8.6±3.0/HP和25.4±3.7/HP（P.001），前组形态异常较其甚（MC多较小，呈多角或不规则状），且组织中可见分散的崩解颗粒。

CPT significantly inhibits the early stages of the Con A-induced T cell activation and proliferation, and detents the T lymphocytes in G0/G1 phase.

CPT可明显抑制ConA刺激的T淋巴细胞的活化、增殖，同时使淋巴细胞阻滞于G0/G1期。

In order to select monomer of Chinese herbal polysaccharide with better immunity, four CHPs, APS, ABPS, IRPS and DOPS at six doses were added in the splenic lymphocyte of chick in vitro, and cultured 48 h. The splenic lymphocyte proliferation was determined with the MTT method.

为了筛选免疫增强作用较好的单味中药成分，将6种浓度的黄芪多糖、牛膝多糖、板蓝根多糖、山药多糖等4种中药多糖分别加入到体外培养的鸡脾脏淋巴细胞中，培养48h后，用MTT法测定淋巴细胞增殖的变化。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。