淋巴细胞

Lymphocyte stimulation index was calculated after counting the flares using β liquid scintilloscope.

同时建立成骨细胞、淋巴细胞混合培养模型，利用β液闪计数仪测定每分钟闪烁数，计算淋巴细胞刺激指数。

119G/L .②T lymphocytes were seperated from the purified speen lymphocytes by filtration through nylon fibre columns.

进一步将脾淋巴细胞过尼龙毛柱获取T淋巴细胞。

Results PE was found to be able to stimulate the proliferation of murine splenocyte in the absence of accessory cells. Accessory cell and its supernatant from AC stimulated with PE was able to enhance the proliferation of lymphocyte induced by PE.

结果 PE在0.01～100ng/ml浓度范围能刺激鼠脾淋巴细胞增殖，PE的丝裂原作用无需粘附细胞的辅助，但粘附细胞及其粘附细胞的PE刺激上清能协同PE对鼠脾淋巴细胞的刺激作用。

Antibody to PE could block this function. In vivo PE could enhance murine splenocyte proliferation but not level of IL-2 production.

PE在体内能使小鼠脾淋巴细胞自发淋转率增高，对ConA刺激的增殖反应增强，但对淋巴细胞自发分泌IL-2和ConA诱导IL-2的产生水平无影响。

The expressions of VEGF mRNA in renal cortex in B and group C increased greatly compared with A group at 8th week(P.01), And the expression was decreased more in C group than that in B group at the 8th week;④The light microscopes results showed that no pathological changes in group A; pathological changes were much obvious in group B:glomerular capillary lumen tumbling,lumens blocked,mesangial region widened,basal lamina thicking,mesenterium base inceased,the volume of glomerulus become large,cell population increased,renal tubule vacuolization, renal interstitium was infiltrated by lots of lymphocyte and mononuclear macrophage; pathological changes in group C was light,only see glomerular capillary lumen lightly stegnosis,few lymphocyte infiltrating.

免疫组化结果显示第8周B组大鼠肾皮质VEGF蛋白含量较A组显著增加(P.01)，C组VEGF含量较B组有明显减少(P.01)，C组较A组表达量仍然增加(P.01)；③第8周B组肾皮质VEGF mRNA表达较A组有明显上调(P.01)，与B组相比，辛伐他汀可以明显减少C组肾皮质VEGF mRNA表达；④光学显微镜下A组肾小球毛细血管腔均匀一致，无狭窄，肾小管-间质无炎症细胞浸润。B组则病变较明显：大鼠肾小球毛细血管袢塌陷，管腔闭塞，系膜区增宽，基膜增厚和系膜基质增多，肾小球体积增大，出现玻璃样变；肾小管尤其是近区小管肿胀、变性、空泡形成，肾间质可见大量淋巴细胞和单核巨噬细胞浸润。C组病变较轻，可见肾小球毛细血管管腔轻度狭窄，肾小管-间质见少量淋巴细胞浸润。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

The methods es-tablished in this paper for preparing tonsillar lymphocytes is simple and practicable.

本文研究和建立的扁桃体淋巴细胞的分离培养方法操作简单，制备的淋巴细胞具有较长的存活时间。

The morphologies and phenotypes of the cells have been changed during cultivation.Conclusion The methods es-tablished in this paper for preparing tonsillar lymphocytes is simple and practicable.

本文研究和建立的扁桃体淋巴细胞的分离培养方法操作简单，制备的淋巴细胞具有较长的存活时间。

Result: Wallerian degeneration was observed in the proximal and distal ends of all grafts of nerves harvested at 2 weeks post-engraftment.There were so many T lymphocyte in group A. 3 and 5 WEEK group is less.none can be seen in 7 and 9 WEEK group.

结果：术后2周，所有移植段的近端、远端均有不同程度Wallerian变性，A组移植段见明显T淋巴细胞浸润，3周，5周组次之，9周及7周组和自体组未见T淋巴细胞。

Results: Wallerian degeneration was observed in the proximal and distal ends of all grafts of nerves harvested at 2 weeks post-engraftment; infiltration of T lymphocytes at the grafts in group B was significant, less T lymphocytes were seen in group C, D and E, while no T lymphocytes were seen in group A and F.

结果：术后2周，所有移植段近端，移植远端神经均有不同程度Wallerian变性，B组移植段见明显T淋巴细胞浸润，C，D，E组次之，A组及F组未见T淋巴细胞。B组以大量成纤维细胞增生为主，A，F组以SC增生为主，C，D，E组介于两者之间。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷