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淋巴细胞

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Phylogenetic analysis of the long terminal repeatand env gene of htlv-1 gave a phylogenetic classification of htlv-1 genotypes into five major molecular subtypes: cosmopolitan, japanese, west african, central african, and melanesian. in order to understand the major genotype of htlv-1 prevalent in china, htlv-1 env gene was amplified by pcr and sequenced from peripheral blood lymphocyts of three htlv-1 carriers in the putian region of fujian province.

根据基因组及血清学反应可将其分为人t淋巴细胞白血病病毒1型(htlv-1)和2型(htlv-2),两型病毒基因组同源性大于60%。htlv-1主要侵染t淋巴细胞,可引起成人t细胞白血病、热带下肢痉挛性瘫痪或htlv-1相关脊髓病,并且在多种自身免疫病患者体内也发现了该病毒的存在。

Objective To investigate the statistical distribution of the data of cytokinesis-block micronucleus test to provide basis of statistical analysis.

目的 探讨微核试验数据(每1000个双核淋巴细胞中具有微核的淋巴细胞数)的统计分布,为微核试验数据的统计分析提供依据。

Methods: Mitotic Index, chromosome aberration and micronucleus test in bone marrow lymphocytes of mice were determined.

采用小鼠骨髓淋巴细胞有丝分裂指数试验、染色体畸变试验、微核试验,观察蒲公英水煎液对小鼠骨髓淋巴细胞的遗传损伤效应的影响。

In contrast, nasal administration of a mixture of MBP6886 and 8799 completely blocked gpMBPinduced EAE even at lower dosage than being used alone. Rats tolerized with MBP6886+8799 nasally showed decreased T cell responses to MBP, reflected by lymphocyte proliferation and IFNγ ELISPOT assays.

淋巴细胞增殖实验和IFNγ ELISPOT检测显示,与鼻黏膜给予大鼠MBP110128组相比,鼻黏膜给予大鼠MBP6886+8799可降低T细胞对于MBP的反应性,淋巴结单核细胞中表达IFNγ和TNFα mRNA的细胞数减少,而两组表达TGFβ及IL4 mRNA的淋巴细胞数都低。

The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.

结果表明:在给予不同光照时间的条件下,随着光照时间的延长,褪黑素的分泌水平受到抑制,导致具有特异免疫功能的淋巴细胞百分率下降,具有非特异免疫功能的中性粒细胞百分率上升,经统计分析表明不同组间的测定结果均存在显着差异;采用24h全天光照,用以模拟机体切除松果腺的情况,再给予一定剂量外源性褪黑素,导致机体内具有特异免疫功能的淋巴细胞百分率上升,具有非特异免疫功能的中性粒细胞百分率下降,经统计分析表明不同组间的测定结果均存在显著差异,并且高剂量褪黑素对机体的影响更为明显。

Objective To investigate the effect of nidus vespae on lymphocyte blastisation in mixed culture system of lymphocyte and pancreatic islet.

目的 初步探索露蜂房对淋巴细胞与胰岛混合培养系统中淋巴细胞转化的影响。

Further skin biopsies taken from one newly developed papule and one old ulcerated plaque showed atypical lymphoid cell epidermotropism with formation of Pautrier's microabscesses and perivascular and intravascular infiltration of atypical lymphocytes in the upper to middle dermis.

住院后一个月,吾人从新生的丘疹及原溃疡斑块处再作切片检查,二处切片病理均显示异常淋巴细胞亲皮浸润及Pautrier微脓疡形成;在上层至中层真皮,则有血管周围及血管内的异常淋巴细胞浸润。

Fig 2 Inverted phase-contrast photomicrograph of immortalized human lymphoid cell line established by in vitro immunization and DNA transfection.

DNA转染淋巴细胞的形态学:转染后第8 d,在倒置相关显微镜下可见转化细胞灶,转化细胞体积较淋巴细胞大,略显大小不一,密度较未转化细胞高,但仍保持典型的淋巴样细胞形态。第2周可见簇状的转化细胞集落,其细胞数量迅速增加。

Imbalance of T lymphocyte subsets exists in PNS patients (CD4 and CD4/CD8 ratio increased and CD8 decreased), and the local pokeberry root I of Zunyi Guizhou decoction may correct it and may be beneficial to CD8 lymphocyte growth in vitro.

PNS患者体内存在T淋巴细胞亚群比例失调,CD4、CD4/CD8明显增高,CD8数量明显降低。遵义本地产垂序商陆煎液有利于CD8、细胞的体外生长,故而能纠正PNS患者的T淋巴细胞亚群比例失调。

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞,并在有或无美洲商陆有丝分裂原存在的情况下培养10天后,收集上清液,采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

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