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In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT-PCR protocol and analysed by polyacrylamide gel electrophoresis.

为检测用EB病毒转化建立的永生性人B淋巴母细胞株中N5,N10-亚甲基四氢叶酸还原酶基因的表达及其cDNA序列,用EB病毒转化人外周血B淋巴细胞,建立永生性细胞株后,从培养细胞中提取总RNA,用RT-PCR法扩增MTHFR基因cDNA的不同片段,进行PAGE电泳分析和cDNA序列测定。

Methods: Affinity chromatography was used to purify B16 HACs.γ-IFN and IL-2 secreting activities were determined with crystal voilet staining and lymphoblast proliferation induced by Con A, respectively. Pecific CTL killing rate was detemined with 3H-TdR incorporation.

CNBrSepharose 4B亲和层析法提纯B16HACs,应用体内免疫法检测HAC的抑瘤作用,结晶紫染色法检测γIFN分泌活性,ConA诱导的淋巴母细胞增殖法检测IL2分泌活性,3HTdR掺入法检测特异性CTL的杀伤活性。

In addition torefine our PCR experiment and isolate B cells,we synthesized high qualityimmunobeads in our own lab.The immunobeads we synthesized weremonosized、highly magnetizable and had high isolation result.

我们在经过了多水平、多侧面的检测和分析后并没有发现在非淋巴系统来源的肿瘤细胞存在Ig基因表达的确凿证据。

Mastitis can be localized or widespread, and the breast's lymphatic system may be involved.

乳腺炎可能是局部的,也可能是扩散的,感染可波及乳房的淋巴系统。

Mastitis can be localize d or widespread, and the breast's lymphatic system may be involved.

乳腺炎可能是局部的,也可能是扩散的,感染可波及乳房的淋巴系统。

Out of 9 animals with hearing loss had these autoantibodies; Endolymphatic hydrops, vasculitis of modiolus, degeneration of the neural elements, distension and thicking of the Reissner's membrane were seen in 46%, 78%, 12%, 21% of the experimental group ears respectively.

实验组32耳中内淋巴积水、蜗轴血管炎、螺旋神经节细胞空泡变性肿胀,前庭膜增厚、折叠的发生率分别是46%、78%、12%、21%,听力损害的12耳均有不同程度的上述病理变化。

Two-Phase 18FFDG PET/CT is helpful in diagnosis,staging and choice of treatment perscription of NPC, also is beneficial in differential diagnosis between NPC and nasopharyngitis ?

双时相PET/CT有助于鼻咽癌的诊断,也有利于鼻咽癌与鼻咽炎症或淋巴增生的鉴别诊断,对鼻咽疾病诊治中有良好的医学前景。

Fig 2 Inverted phase-contrast photomicrograph of immortalized human lymphoid cell line established by in vitro immunization and DNA transfection.

DNA转染淋巴细胞的形态学:转染后第8 d,在倒置相关显微镜下可见转化细胞灶,转化细胞体积较淋巴细胞大,略显大小不一,密度较未转化细胞高,但仍保持典型的淋巴样细胞形态。第2周可见簇状的转化细胞集落,其细胞数量迅速增加。

LEC of the salivary gland is a radiosensitive disease and has a better prognosis among the undifferentiated carcinoma at the head and neck region.

结论,唾液腺的淋巴上皮癌在头颈部的未分化癌中的预后是比较好。

G/mL. This phenomenon was due to the milder concentration decline of rutin in lymphatic system.

g/mL的2倍。造成这个现象的原因是由於在淋巴系统中,芸香素的浓度降低幅度较在血循系统中来得缓慢。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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